Induction of GPR40 positively regulates cell motile and growth activities in breast cancer MCF-7 cells

Free fatty acid (FFA) receptors belong to a member of G-protein-coupled receptors. GPCR 120 (GPR120) and GPR40 are identified as FFA receptors and activated via the binding of long- and medium-chain FFAs. The aim of this study was to assess the effects of GPR120 and GPR40 on cell motility and growth...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of receptors and signal transduction Vol. 38; no. 4; pp. 311 - 315
Main Authors:	Fukushima, Kaori, Takahashi, Kaede, Kusaka, Mirai, Ishimoto, Kaichi, Minami, Kanako, Otagaki, Shiho, Fukushima, Nobuyuki, Honoki, Kanya, Tsujiuchi, Toshifumi
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 04-07-2018
Subjects:	Benzoates - pharmacology breast cancer cells Breast Neoplasms - drug therapy Breast Neoplasms - genetics Breast Neoplasms - pathology cell motility Cell Movement - drug effects Cell Proliferation - drug effects Female Gene Expression Regulation, Neoplastic - drug effects GPR40 Humans MCF-7 Cells Pyrimidines - pharmacology Receptors, G-Protein-Coupled - genetics tamoxifen Tamoxifen - pharmacology cell motility breast cancer cells GPR40 tamoxifen
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Free fatty acid (FFA) receptors belong to a member of G-protein-coupled receptors. GPCR 120 (GPR120) and GPR40 are identified as FFA receptors and activated via the binding of long- and medium-chain FFAs. The aim of this study was to assess the effects of GPR120 and GPR40 on cell motility and growth in breast cancer cells treated with tamoxifen (TAM). MCF-7 cells were continuously treated with TAM for approximately 6 months. The expression level of GPR40 gene was markedly higher in the long-term TAM treated (MCF-TAM) cells than in MCF-7 cells. In cell motility assay, MCF-TAM cells indicated the high cell motile activity, compared with MCF-7 cells. The cell motile activity of MCF-TAM cells was suppressed by a selective GPR40 antagonist, GW1100. To evaluate the effects of GPR40 on cell growth activity under estrogen-free conditions, cells were maintained in serum-free DMEM without phenol red for 2 days. In estrogen-free conditioned medium, the cell growth rate of MCF-TAM cells was significantly higher than that of MCF-7 cells. In addition, treatment of GW1100 reduced the cell growth rate of MCF-TAM cells. These results suggest that the cell motile and growth activities may be positively regulated through the induction of GPR40 by the long-term TAM treatment in MCF-7 cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1079-9893 1532-4281
DOI:	10.1080/10799893.2018.1494742