Search Results - "Kurpiewski, Michael R."

ESR spectroscopy identifies inhibitory Cu2+ sites in a DNA-modifying enzyme to reveal determinants of catalytic specificity by Yang, Zhongyu, Kurpiewski, Michael R, Ji, Ming, Townsend, Jacque E, Mehta, Preeti, Jen-Jacobson, Linda, Saxena, Sunil

“…The relationship between DNA sequence recognition and catalytic specificity in a DNA-modifying enzyme was explored using paramagnetic Cu2+ ions as probes for…”
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Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution by Ghosh, Shreya, Lawless, Matthew J, Brubaker, Hanna J, Singewald, Kevin, Kurpiewski, Michael R, Jen-Jacobson, Linda, Saxena, Sunil

“…Abstract Electron paramagnetic resonance (EPR) has become an important tool to probe conformational changes in nucleic acids. An array of EPR labels for…”
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Recognition of the Pro-mutagenic Base Uracil by Family B DNA Polymerases from Archaea by Shuttleworth, Gillian, Fogg, Mark J., Kurpiewski, Michael R., Jen-Jacobson, Linda, Connolly, Bernard A.

“…Archaeal family B DNA polymerases contain a specialised pocket that binds tightly to template-strand uracil, causing the stalling of DNA replication. The…”
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Thermodynamic and Structural Basis for Relaxation of Specificity in Protein–DNA Recognition by Sapienza, Paul J., Niu, Tianyi, Kurpiewski, Michael R., Grigorescu, Arabela, Jen-Jacobson, Linda

“…As a novel approach to the structural and functional properties that give rise to extremely stringent sequence specificity in protein–DNA interactions, we have…”
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Mechanisms of Coupling between DNA Recognition Specificity and Catalysis in EcoRI Endonuclease by Kurpiewski, Michael R., Engler, Lisa E., Wozniak, Lucyna A., Kobylanska, Anna, Koziolkiewicz, Maria, Stec, Wojciech J., Jen-Jacobson, Linda

“…Proteins that bind to specific sites on DNA often do so in order to carry out catalysis or specific protein-protein interaction while bound to the recognition…”
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The Energetic Basis of Specificity in the Eco RI Endonuclease-DNA Interaction by Lesser, David R., Kurpiewski, Michael R., Jen-Jacobson, Linda

“…High sequence selectivity in DNA-protein interactions was analyzed by measuring discrimination by Eco RI endonuclease between the recognition site GAATTC and…”
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Facilitated Distortion of the DNA Site Enhances EcoRI Endonuclease-DNA Recognition by Lesser, David R., Kurpiewski, Michael R., Waters, Timothy, Connolly, Bernard A., Jen-Jacobson, Linda

“…We have measured the binding of EcoRI endonuclease to a complete set of purine-base analogue sites, each of which deletes one functional group that forms a…”
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Chiral Phosphorothioates as Probes of Protein Interactions with Individual DNA Phosphoryl Oxygens:  Essential Interactions of EcoRI Endonuclease with the Phosphate at pGAATTC by Kurpiewski, Michael R, Koziolkiewicz, Maria, Wilk, Andrzej, Stec, Wojciech J, Jen-Jacobson, Linda

“…The contact between EcoRI endonuclease and the “primary clamp” phosphate of its recognition site pGAATTC is absolutely required for recognition of the…”
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ESR spectroscopy identifies inhibitory Cu²⁺ sites in a DNA-modifying enzyme to reveal determinants of catalytic specificity by Yang, Zhongyu, Kurpiewski, Michael R., Ji, Ming, Townsend, Jacque E., Mehta, Preeti, Jen-Jacobson, Linda, Saxena, Sunil

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ESR spectroscopy identifies inhibitory Cu 2+ sites in a DNA-modifying enzyme to reveal determinants of catalytic specificity by Yang, Zhongyu, Kurpiewski, Michael R., Ji, Ming, Townsend, Jacque E., Mehta, Preeti, Jen-Jacobson, Linda, Saxena, Sunil

“…The relationship between DNA sequence recognition and catalytic specificity in a DNA-modifying enzyme was explored using paramagnetic Cu 2+ ions as probes for…”
Get full text

ESR spectroscopy identifies inhibitory Cu^sup 2+^ sites in a DNA-modifying enzyme to reveal determinants of catalytic specificity by Yang, Zhongyu, Kurpiewski, Michael R, Ji, Ming, Townsend, Jacque E, Mehta, Preeti, Jen-Jacobson, Linda, Saxena, Sunil

“…The relationship between DNA sequence recognition and catalytic specificity in a DNA-modifying enzyme was explored using paramagnetic ... ions as probes for…”
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Thermodynamic Parameters of Specific and Nonspecific Protein-DNA Binding by Jen-jacobson, Linda, Engler, Lisa E., Ames, Jennifer T., Kurpiewski, Michael R., Grigorescu, Arabela

“…Proteins that bind preferentially to specific recognition sites on DNA also bind more weakly to nonspecific DNA. We have studied both specific and non-specific…”
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Structural adaptations in the interaction of EcoRI endonuclease with methylated GAATTC sites by Jen‐Jacobson, L., Engler, L. E., Lesser, D. R., Kurpiewski, M. R., Yee, C., McVerry, B.

“…We have studied the interaction of EcoRI endonuclease with oligonucleotides containing GAATTC sites bearing one or two adenine‐N6‐methyl groups, which would be…”
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Assay of restriction endonucleases using oligonucleotides by Connolly, B A, Liu, H H, Parry, D, Engler, L E, Kurpiewski, M R, Jen-Jacobson, L

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Stereoselective interaction with chiral phosphorothioates at the central DNA kink of the EcoRI endonuclease-GAATTC complex by LESSER, D. R, GRAJKOWSKI, A, KURPIEWSKI, M. R, KOZIOLKIEWICZ, M, STEC, W. J, JEN-JACOBSON, L

“…We have probed the contacts between EcoRI endonuclease and the central phosphate of its recognition site GAApTTC, using synthetic oligonucleotides containing…”
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Chiral Phosphorothioates as Probes of Protein Interactions with Individual DNA Phosphoryl Oxygens:  Essential Interactions of Eco RI Endonuclease with the Phosphate at pGAATTC by Kurpiewski, Michael R., Koziolkiewicz, Maria, Wilk, Andrzej, Stec, Wojciech J., Jen-Jacobson, Linda

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Proteases of the nematode Caenorhabditis elegans by Sarkis, G J, Kurpiewski, M R, Ashcom, J D, Jen-Jacobson, L, Jacobson, L A

“…Crude homogenates of the soil nematode Caenorhabditis elegans exhibit strong proteolytic activity at acid pH. Several kinds of enzyme account for much of this…”
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