Effects of dietary silkrose of Antheraea yamamai on gene expression profiling and disease resistance to Edwardsiella tarda in Japanese medaka (Oryzias latipes)

Effects of dietary silkrose of Antheraea yamamai on gene expression profiling and disease resistance to Edwardsiella tarda in Japanese medaka (Oryzias latipes)

We previously identified a novel acidic polysaccharide, silkrose-AY, from the Japanese oak silkmoth (Antheraea yamamai), which can activate an innate immune response in mouse macrophage cells. However, innate immune responses stimulated by silkrose-AY in teleosts remain unclear. Here, we show the in...

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Bibliographic Details
Published in:Fish & shellfish immunology Vol. 114; pp. 207 - 217
Main Authors: Ali, Muhammad Fariz Zahir, Kameda, Kenta, Kondo, Fumitaka, Iwai, Toshiharu, Kurniawan, Rio Aditya, Ohta, Takashi, Ido, Atsushi, Takahashi, Takayuki, Miura, Chiemi, Miura, Takeshi
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-07-2021
Subjects:
Immune response
Immunostimulant
Insect
Intestine
Japanese medaka
Microarray
Polysaccharide
Immunostimulant
Immune response
Intestine
Japanese medaka
Microarray
Polysaccharide
Insect
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Summary:We previously identified a novel acidic polysaccharide, silkrose-AY, from the Japanese oak silkmoth (Antheraea yamamai), which can activate an innate immune response in mouse macrophage cells. However, innate immune responses stimulated by silkrose-AY in teleosts remain unclear. Here, we show the influence of dietary silkrose-AY in medaka (Oryzias latipes), a teleost model, in response to Edwardsiella tarda infection. Dietary silkrose-AY significantly improved the survival of fish and decreased the number of bacteria in their kidneys after the fish were artificially infected with E. tarda by immersion. We also performed a microarray analysis of the intestine, which serves as a primary barrier against microbial infection, to understand the profiles of differentially expressed genes (DEGs) evoked by silkrose-AY. The dietary silkrose-AY group showed differential expression of 2930 genes when compared with the control group prior to E. tarda infection. Gene ontology and pathway analysis of the DEGs highlighted several putative genes involved in pathogen attachment/recognition, the complement and coagulation cascade, antimicrobial peptides/enzymes, opsonization/phagocytosis, and epithelial junctional modification. Our findings thus provide fundamental information to help understand the molecular mechanism of bacterial protection offered by insect-derived immunostimulatory polysaccharides in teleosts. •Effects of dietary silkrose of Antheraea yamamai (silkrose-AY) on Japanese medaka were investigated.•Silkrose-AY significantly improves the survival rate of Japanese medaka in response to Edwardsiella tarda infection.•Silkrose-AY decreases the number of bacteria in the medaka kidneys after infected with E. tarda.•Innate immune responses were stimulated in the intestine of the fish fed with silkrose-AY.
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ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2021.05.001