Search Results - "Kurkela, Riitta"

Rat acid phosphatase: overexpression of active, secreted enzyme by recombinant baculovirus-infected insect cells, molecular properties, and crystallization by Vihko, P. (University of Oulu, Oulu, Finland), Kurkela, R, Porvari, K, Herrala, A, Lindfors, A, Lindqvist, Y, Schneider, G

“…Rat prostatic acid phosphatase (rPAP; orthophosphoric-monoester phosphohydrolase (acid optimum), EC (3.1.3.2) was expressed in the baculovirus expression…”
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Production, purification, and functional analysis of recombinant human and mouse 17β-hydroxysteroid dehydrogenase type 7 by Törn, Svea, Nokelainen, Pasi, Kurkela, Riitta, Pulkka, Anitta, Menjivar, Marta, Ghosh, Sikha, Coca-Prados, Miguel, Peltoketo, Hellevi, Isomaa, Veli, Vihko, Pirkko

“…17β-Hydroxysteroid dehydrogenases (17HSDs) have a central role in the regulation of the biological activity of sex steroid hormones. There is increasing…”
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Investigating the effect of VEGF glycosylation on glycosaminoglycan binding and protein unfolding by Brandner, Barbara, Kurkela, Riitta, Vihko, Pirkko, Kungl, Andreas J.

“…VEGF 165 binding to endothelial cells is potentiated by glycosaminoglycans (GAGs). Here, we have investigated the impact of VEGF 165 N-glycosylation on GAG…”
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Sex Hormone Metabolism in Prostate Cancer Cells during Transition to an Androgen-Independent State by Härkönen, Päivi, Törn, Svea, Kurkela, Riitta, Porvari, Katja, Pulkka, Anitta, Lindfors, Aija, Isomaa, Veli, Vihko, Pirkko

“…The progression of prostate cancer during androgen deprivation therapy is a serious clinical problem. Little is known, however, about the mechanisms behind the…”
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USF2 is connected to GAAAATATGATA element and associates with androgen receptor-dependent transcriptional regulation in prostate by Kivinen, Anne, Patrikainen, Lila, Kurkela, Riitta, Porvari, Katja, Vihko, Pirkko

“…BACKGROUND We have previously identified a GAAAATATGATA binding site (pros) of a transcription factor involved in prostatic and androgen‐dependent gene…”
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Production, purification, and functional analysis of recombinant human and mouse 17beta-hydroxysteroid dehydrogenase type 7 by Törn, Svea, Nokelainen, Pasi, Kurkela, Riitta, Pulkka, Anitta, Menjivar, Marta, Ghosh, Sikha, Coca-Prados, Miguel, Peltoketo, Hellevi, Isomaa, Veli, Vihko, Pirkko

“…17beta-Hydroxysteroid dehydrogenases (17HSDs) have a central role in the regulation of the biological activity of sex steroid hormones. There is increasing…”
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Ligand Affinity, Homodimerization, and Ligand-Induced Secondary Structural Change of the Human Vitamin D Receptor by Falsone, S.Fabio, Kurkela, Riitta, Chiarandini, Gianpiero, Vihko, Pirkko, Kungl, Andreas J.

“…The intrinsic tryptophan fluorescence signal of the full-length nuclear receptor hVDR was used to directly determine the dissociation constants, Kd, of two…”
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Expression of Active, Secreted Human Prostate-specific Antigen by Recombinant Baculovirus-infected Insect Cells on a Pilot-scale by Kurkela, Riitta, Herrala, Annakaisa, Henttu, Pirkko, Nal, Heini, Vihko, Pirkko

“…We have expressed human prostate-specific antigen (PSA) on a pilot-scale in Spodoptera frugiperda Sf9 insect cells using recombinant baculovirus system…”
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Human prostate-specific glandular kallikrein is expressed as an active and an inactive protein by Herrala, Annakaisa, Kurkela, Riitta, Porvari, Katja, Isomaki, Ritva, Henttu, Pirkko, Vihko, Pirkko

“…A polymorphism in the human prostate-specific glandular kallikrein (hKLK2) gene was described by direct sequencing (by PCR) of genomic DNAs isolated from…”
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Site-directed mutagenesis of prostatic acid phosphatase. Catalytically important aspartic acid 258, substrate specificity, and oligomerization by Porvari, K S, Herrala, A M, Kurkela, R M, Taavitsainen, P A, Lindqvist, Y, Schneider, G, Vihko, P T

“…At the active site of rat prostatic acid phosphatase (rPAP), residue Asp258 is a suitable candidate to act as an acid/base catalyst during phosphoester…”
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Differential Androgen Regulation of Rat Prostatic Acid Phosphatase Transcripts by Porvari, K., Kurkela, R., Kivinen, A., Vihko, P.

“…Steady-state levels of the mRNAs encoding rat prostatic acid phosphatase (rPAP) were measured from the ventral prostate of rats after castration and…”
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Androgen‐sensitive human prostate cancer cells, LNCaP, produce both N‐terminally mature and truncated prostate‐specific antigen isoforms by Herrala, Annakaisa, Kurkela, Riitta, Vihinen, Mauno, Kalkkinen, Nisse, Vihko, Pirkko

“…To characterize prostate‐specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were…”
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Time-resolved immunofluorometric assay of human prostate-specific antigen by Vihko, P, Kurkela, R, Ramberg, J, Pelkonen, I, Vihko, R

“…Assay of human serum prostate-specific antigen (PSA) is gaining importance in diagnosis and follow-up of prostatic cancer. In this time-resolved…”
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