Inhibitory potential of EGCG on Streptococcus mutans biofilm: A new approach to prevent Cariogenesis

Dental caries is a common cause for tooth loss and Streptococcus mutans is identified as the etiologic pathogen. This study evaluates the inhibitory potential of Epigallocatechin gallate (EGCG) on S.mutans glucansucrase enzyme and its biofilm. Glucansucrase binding and the inhibitory potential of EG...

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Published in:	Microbial pathogenesis Vol. 143; p. 104129
Main Authors:	Hairul Islam, Mohamed Ibrahim, Arokiyaraj, Selvaraj, Kuralarasan, M., Senthil Kumar, Venugopal, Harikrishnan, Pandurangan, Saravanan, Subramanian, Ashok, Ganapathy, Chellappandian, Muthiah, Bharanidharan, Rajaraman, Muralidaran, Senguttuvan, Thirugnanasambantham, Krishnaraj
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-06-2020
Subjects:	Biofilm Biofilms - drug effects Catechin - analogs & derivatives Catechin - pharmacology Catechin - therapeutic use Dental caries Dental Caries - microbiology Dental Caries - prevention & control EGCG Glucansucrase Humans Microscopy, Electron, Scanning Molecular Docking Simulation Streptococcus mutans Streptococcus mutans - drug effects Streptococcus mutans - ultrastructure Tooth - microbiology Biofilm Dental caries Glucansucrase EGCG Streptococcus mutans
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Summary:	Dental caries is a common cause for tooth loss and Streptococcus mutans is identified as the etiologic pathogen. This study evaluates the inhibitory potential of Epigallocatechin gallate (EGCG) on S.mutans glucansucrase enzyme and its biofilm. Glucansucrase binding and the inhibitory potential of EGCG was validated using AutoDock tool and enzyme inhibitory assay. Biofilm inhibitory potential was also confirmed using Scanning Electron Microscopic (SEM) analysis in human tooth samples. Molecular docking revealed that EGCG interacted with GLU 515 and TRP 517 amino acids and binds to glucansucrase. SEM analysis revealed inhibition of S.mutans biofilm by various concentrations of EGCG on surfaces of tooth samples. Bioinformatics and biological assays confirmed that EGCG potentially binds to the S. mutans glucansucrase and inhibits its enzymatic activity. Enzymatic inhibition of glucansucrase attenuated biofilm formation potential of S. mutans on tooth surface. Thus, we conclude that EGCG inhibitory potential of S. mutans biofilm on the tooth surface is a novel approach in prevention of dental caries. •Bioinformatics analysis revealed glucansucrase enzyme binding potential of EGCG.•EGCG inhibited glucansucrase enzyme activity.•SEM analysis revealed EGCG inhibited S.mutans biofilm on surfaces of tooth.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0882-4010 1096-1208
DOI:	10.1016/j.micpath.2020.104129