Search Results - "Kupcho, Kevin R."

Target engagement and drug residence time can be observed in living cells with BRET by Robers, Matthew B., Dart, Melanie L., Woodroofe, Carolyn C., Zimprich, Chad A., Kirkland, Thomas A., Machleidt, Thomas, Kupcho, Kevin R., Levin, Sergiy, Hartnett, James R., Zimmerman, Kristopher, Niles, Andrew L., Ohana, Rachel Friedman, Daniels, Danette L., Slater, Michael, Wood, Monika G., Cong, Mei, Cheng, Yi-Qiang, Wood, Keith V.

“…The therapeutic action of drugs is predicated on their physical engagement with cellular targets. Here we describe a broadly applicable method using…”
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Tumor-suppressor role of Notch3 in medullary thyroid carcinoma revealed by genetic and pharmacological induction by Jaskula-Sztul, Renata, Eide, Jacob, Tesfazghi, Sara, Dammalapati, Ajitha, Harrison, April D, Yu, Xiao-Min, Scheinebeck, Casi, Winston-McPherson, Gabrielle, Kupcho, Kevin R, Robers, Matthew B, Hundal, Amrit K, Tang, Weiping, Chen, Herbert

“…Notch1-3 are transmembrane receptors that appear to be absent in medullary thyroid cancer (MTC). Previous research has shown that induction of Notch1 has a…”
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Simultaneous Monitoring of Discrete Binding Events Using Dual-Acceptor Terbium-Based LRET by Kupcho, Kevin R, Stafslien, Deborah K, DeRosier, Therese, Hallis, Tina M, Ozers, Mary Szatkowski, Vogel, Kurt W

“…Lanthanide resonance energy transfer (LRET) between chelated terbium and two spectrally distinct acceptor fluorophores (fluorescein and Alexa Fluor 633) is…”
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The Androgen Receptor T877A Mutant Recruits LXXLL and FXXLF Peptides Differently than Wild-Type Androgen Receptor in a Time-Resolved Fluorescence Resonance Energy Transfer Assay by Ozers, Mary Szatkowski, Marks, Bryan D, Gowda, Krishne, Kupcho, Kevin R, Ervin, Kerry M, De Rosier, Therese, Qadir, Naveeda, Eliason, Hildegard C, Riddle, Steven M, Shekhani, Mohammed Saleh

“…The interactions of the ligand binding domain (LBD) of androgen receptor (AR) and the AR T877A mutant, found in prostate cancer, with peptides from coactivator…”
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Characterization of Phosphotyrosine Binding Motifs in the Cytoplasmic Domain of Platelet/Endothelial Cell Adhesion Molecule-1 (PECAM-1) That Are Required for the Cellular Association and Activation of the Protein-tyrosine Phosphatase, SHP-2 by Jackson, Denise E., Kupcho, Kevin R., Newman, Peter. J.

“…Recent studies have shown that the Src homology-2 (SH2) domain-containing protein-tyrosine phosphatase, SHP-2, associates with the cytoplasmic domain of…”
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Overcoming compound interference in fluorescence polarization-based kinase assays using far-red tracers by Vedvik, Kevin L, Eliason, Hildegard C, Hoffman, Randy L, Gibson, Jasmin R, Kupcho, Kevin R, Somberg, Richard L, Vogel, Kurt W

“…Kinase-mediated phosphorylation of proteins is critical to the regulation of many biological processes, including cell growth, apoptosis, and differentiation…”
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Differential T Cell Responsiveness to Proliferative Stimuli Assessed with a Novel, Homogeneous Ki-67 Immunoassay by Lazar, Dan F, Kupcho, Kevin R., Niles, Andrew L., Cali, James J.

“…Proliferative capacity is an important component of T cell fitness and a relevant parameter for characterization of CAR T cells and T cell activating agents…”
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Abstract 1933: Improved DAMP assays for the in vitro assessment of immunogenic cell death by Niles, Andrew L., Kupcho, Kevin R., Lazar, Dan F., Cali, James J.

“…The extracellular levels of damage-associated molecular patterns (DAMPs) released during immunogenic cell death (ICD) are positively correlated with the…”
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Abstract 1832: A real-time extracellular ATP assay to identify modulators of the innate immune response by Niles, Andrew L., Kupcho, Kevin R., Lazar, Dan F., Cali, James J.

“…Damage-associated molecular patterns (DAMPs) liberated or exposed by dying cells after chemotherapeutic treatment augment innate immune responses and improve…”
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Abstract 710: Real-time apoptosis and necrosis detection in 3D spheroid cellmodels by Niles, Andrew L., Kupcho, Kevin R., Riss, Terry L., Lazar, Dan F., Cali, James J.

“…The structural and biological complexity of cancer spheroids make them uniquely suited to address or approximate the pharmacodynamics of new chemical entities…”
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Abstract 1631: A homogeneous assay system for discernment of proliferative, antiproliferative, and cytotoxic effects in culture by Lazar, Dan F., Kupcho, Kevin R., Niles, Andrew L., Cali, James J.

“…We describe a novel homogeneous cell-based assay developed for the quantitative detection of Ki-67 protein, a recognized marker of cell proliferation,…”
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Abstract 5595: Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F., Kupcho, Kevin R., Sondgeroth, Casey A., Thompson, David V., O'Brien, Martha A., Gilden, Julia K., Hsiao, Kevin, Cali, James J.

“…Cytokines play a major role in cancer biology as inflammatory and immunomodulatory agents and are frequently measured in cell culture models and other…”
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Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F, Kupcho, Kevin R, Sondgeroth, Casey A, Hsiao, Kevin, Thompson, David V, O’Brien, Martha A, Gilden, Julia K, Cali, James J

“…We have utilized NanoLuc® Binary Technology (NanoBiT®) to develop a homogeneous and rapid assay method (≤ 70 min completion time) to measure cytokines released…”
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Abstract 1428: Luminescent immunoassays for homogeneous determination of cell proliferation and senescence by Lazar, Dan F., Kupcho, Kevin R., Niles, Andrew L., Cali, James J.

“…We have developed homogeneous, luminescent immunoassays for simple and rapid determination of Ki-67 and uPAR levels in 96-well format, thereby enabling…”
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Abstract 4784: Selective bioluminogenic HDAC activity assays for profiling HDAC inhibitors by Kupcho, Kevin R., Evans, Nathan J., Niles, Andrew L., Kirkland, Thomas A., Lazar, Dan F.

“…Histone deacetylases (HDACs) play critical roles in the regulation of gene transcription and cell signaling events by deacetylating histones and other…”
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Abstract 4237: Isoenzyme-selective HDAC activity assays by Kupcho, Kevin R., Evans, Nathan J., Niles, Andrew L., Lazar, Dan F., Kirkland, Thomas A.

“…Histone deacetylases (HDACs) are the family of enzymes which catalyze removal of acetyl groups from lysine residues on both histone and non-histone proteins…”
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Abstract 3731: Luminescent cell health assays for tumor spheroid evaluation by Valley, Michael P., Kupcho, Kevin R., Zimprich, Chad A., Niles, Andrew L., Cali, James J., Kelm, Jens M., Moritz, Wolfgang, Lazar, Dan F.

“…Microtissues produced in 3D cell culture are much more representative of actual living tissue compared to monolayers produced in 2D cell culture. In fact, in…”
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Fluorescent cascade and direct assays for characterization of RAF signaling pathway inhibitors by Kupcho, Kevin R, Bruinsma, Rica, Hallis, Tina M, Lasky, David A, Somberg, Richard L, Turek-Etienne, Tammy, Vogel, Kurt W, Huwiler, Kristin G

“…RAF kinases are part of a conserved signaling pathway that impacts cell growth, differentiation, and survival, and RAF pathway dysregulation is an attractive…”
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Fluorescent Cascade and Direct Assays for Characterization of RAF Signaling Pathway Inhibitors by Kupcho, Kevin R, Bruinsma, Rica, Hallis, Tina M, Lasky, David A, Somberg, Richard L, Turek-Etienne, Tammy, Vogel, Kurt W, Huwiler, Kristin G

“…RAF kinases are part of a conserved signaling pathway that impacts cell growth, differentiation, and survival, and RAF pathway dysregulation is an attractive…”
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