Effective mRNA pulmonary delivery by dry powder formulation of PEGylated synthetic KL4 peptide
[Display omitted] •PEG12KL4 peptide is a promising mRNA delivery vector for pulmonary delivery.•Inhalable mRNA dry powder can be prepared by spray drying and spray freeze drying.•PEG12KL4/mRNA formulations did not cause significant toxicity and inflammatory response. Pulmonary delivery of messenger...
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| Published in: | Journal of controlled release Vol. 314; pp. 102 - 115 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Netherlands
Elsevier B.V
28-11-2019
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | [Display omitted]
•PEG12KL4 peptide is a promising mRNA delivery vector for pulmonary delivery.•Inhalable mRNA dry powder can be prepared by spray drying and spray freeze drying.•PEG12KL4/mRNA formulations did not cause significant toxicity and inflammatory response.
Pulmonary delivery of messenger RNA (mRNA) has considerable potential as therapy or vaccine for a range of lung diseases. Inhaled dry powder formulation of mRNA is particularly attractive as it has superior stability and dry powder inhaler is relatively easy to use. A safe and effective mRNA delivery vector as well as a suitable particle engineering method are required to produce a dry powder formulation that is respirable and mediates robust transfection in the lung. Here, we introduce a novel RNA delivery vector, PEG12KL4, in which the synthetic cationic KL4 peptide is attached to a monodisperse linear PEG of 12-mers. The PEG12KL4 formed nano-sized complexes with mRNA at 10:1 ratio (w/w) and mediated effective transfection on human lung epithelial cells. PEG12KL4/mRNA complexes were successfully formulated into dry powder by spray drying (SD) and spray freeze drying (SFD) techniques. Both SD and SFD powder exhibited satisfactory aerosol properties for inhalation. More importantly, the biological activity of the PEG12KL4 /mRNA complexes were successfully preserved after drying. Using luciferase mRNA, the intratracheal administration of the liquid or powder aerosol of PEG12KL4 /mRNA complexes at a dose of 5μg mRNA resulted in luciferase expression in the deep lung region of mice 24h post-transfection. The transfection efficiency was superior to naked mRNA or lipoplexes (Lipofectamine 2000), in which luciferase expression was weaker and restricted to the tracheal region only. There was no sign of inflammatory response or toxicity of the PEG12KL4 /mRNA complexes after single intratracheal administration. Overall, PEG12KL4 is an excellent mRNA transfection agent for pulmonary delivery. This is also the first study that successfully demonstrates the preparation of inhalable dry powder mRNA formulations with in vivo transfection efficiency, showing the great promise of PEG12KL4 peptide as a mRNA delivery vector candidate for clinical applications. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0168-3659 1873-4995 |
| DOI: | 10.1016/j.jconrel.2019.10.026 |