Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis

Spleen stromal lines which support in vitro hematopoiesis are investigated for their lineage origin and hematopoietic support function in vivo. Marker expression and gene profiling identify a lineage relationship with mesenchymal stem cells and perivascular reticular cells described recently in bone...

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Bibliographic Details
Published in:	PloS one Vol. 14; no. 10; p. e0223416
Main Authors:	O'Neill, Helen C, Lim, Hong K, Periasamy, Pravin, Kumarappan, Lavanya, Tan, Jonathan K H, O'Neill, Terence J
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 04-10-2019 Public Library of Science (PLoS)
Subjects:	Adipogenesis - genetics Animals Antigens Biology and Life Sciences Biomarkers Biomedical materials Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Cell Differentiation Computational Biology - methods Criminal investigation CXCL12 protein EDTA Gene expression Gene Expression Profiling Genes Genetic aspects Grafts Health aspects Hematopoiesis Hematopoiesis, Extramedullary Hematopoietic Stem Cells - cytology Heparan sulfate In vivo methods and tests Kidneys Medicine Medicine and Health Sciences Mesenchyme Mice Mice, Inbred NOD Mice, SCID Myeloid cells Myelopoiesis Myelopoiesis - genetics Osteogenesis Osteosarcoma Platelet-derived growth factor Research and Analysis Methods Spleen Spleen - cytology Stem cell transplantation Stem cells Stromal cells Stromal Cells - cytology Stromal Cells - transplantation Tissue engineering Transcriptome Singapore Queensland Australia Australia Canberra Australian Capital Territory Australia Australian Capital Territory Australia
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Summary:	Spleen stromal lines which support in vitro hematopoiesis are investigated for their lineage origin and hematopoietic support function in vivo. Marker expression and gene profiling identify a lineage relationship with mesenchymal stem cells and perivascular reticular cells described recently in bone marrow. Stromal lines commonly express Cxcl12, Pdgfra and Pdgfr typical of bone marrow derived perivascular reticular cells but reflect a unique cell type in terms of other gene and marker expression. Their classification as osteoprogenitors is confirmed through ability to undergo osteogenic, but not adipogenic or chondrogenic differentiation. Some stromal lines were shown to form ectopic niches for HSCs following engraftment under the kidney capsule of NOD/SCID mice. The presence of myeloid cells and a higher representation of a specific dendritic-like cell type over other myeloid cells within grafts was consistent with previous in vitro evidence of hematopoietic support capacity. These studies reinforce the role of perivascular/perisinusoidal reticular cells in hematopoiesis and implicate such cells as niches for hematopoiesis in spleen.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0223416