3D modelling of the pathogenic Leptospira protein LipL32: A bioinformatics approach

[Display omitted] •LipL32 in pathogenic Leptospira species share a common branch in phylogeny tree.•LipL32 pairwise alignment showed high conservation among their homologs.•LipL32 as a strong hydrophobic protein.•Signal peptide of LipL32 was present at the N-terminus.•LipL32 as a target gene that ma...

Full description

Saved in:

Bibliographic Details
Published in:	Acta tropica Vol. 176; pp. 433 - 439
Main Authors:	Kumaran, Sharmilah Kumari, Bakar, Mohd. Faizal Abu, Mohd-Padil, Hirzahida, Mat-Sharani, Shuhaila, Sakinah, S., Poorani, K., Alsaeedy, Hiba, Peli, Amira, Wei, Teh Seoh, Ling, Mok Pooi, Hamat, Rukman Awang, Neela, Vasantha Kumari, Higuchi, Akon, Alarfaj, Abdullah A., Rajan, Mariappan, Benelli, Giovanni, Arulselvan, Palanisamy, Kumar, S. Suresh
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-2017
Subjects:	3D modeling Antigens, Bacterial - chemistry Antigens, Bacterial - immunology Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - immunology Computational Biology Diagnostic Genomics Leptospira - genetics Leptospira - immunology Leptospirosis - immunology LipL32 Lipoproteins - chemistry Lipoproteins - immunology Models, Molecular Pathogenic leptospires Target gene 3D modeling Diagnostic Pathogenic leptospires LipL32 Genomics Target gene
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	[Display omitted] •LipL32 in pathogenic Leptospira species share a common branch in phylogeny tree.•LipL32 pairwise alignment showed high conservation among their homologs.•LipL32 as a strong hydrophobic protein.•Signal peptide of LipL32 was present at the N-terminus.•LipL32 as a target gene that may be used as antigen to detect Leptospira. Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira species (Leptospiraceae). LipL32 is an abundant lipoprotein from the outer membrane proteins (OMPs) group, highly conserved among pathogenic and intermediate Leptospira species. Several studies used LipL32 as a specific gene to identify the presence of leptospires. This research was aimed to study the characteristics of LipL32 protein gene code, to fill the knowledge gap concerning the most appropriate gene that can be used as antigen to detect the Leptospira. Here, we investigated the features of LipL32 in fourteen Leptospira pathogenic strains based on comparative analyses of their primary, secondary structures and 3D modeling using a bioinformatics approach. Furthermore, the physicochemical properties of LipL32 in different strains were studied, shedding light on the identity of signal peptides, as well as on the secondary and tertiary structure of the LipL32 protein, supported by 3D modelling assays. The results showed that the LipL32 gene was present in all the fourteen pathogenic Leptospira strains used in this study, with limited diversity in terms of sequence conservation, hydrophobic group, hydrophilic group and number of turns (random coil). Overall, these results add basic knowledge to the characteristics of LipL32 protein, contributing to the identification of potential antigen candidates in future research, in order to ensure prompt and reliable detection of pathogenic Leptospira species.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0001-706X 1873-6254
DOI:	10.1016/j.actatropica.2017.09.011