Antifungal and Mechanical Properties of Tissue Conditioner Containing Plant‐Derived Component: An In Vitro Study

Antifungal and Mechanical Properties of Tissue Conditioner Containing Plant‐Derived Component: An In Vitro Study

Purpose To evaluate the antifungal activity and mechanical properties of a novel antifungal tissue conditioner containing Juncus powder. Materials and Methods Juncus powder was mixed with GC tissue conditioner at concentrations of 2.5%, 5.0%, and 10.0% by mass. The cylindrical specimens of Juncus‐mi...

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Published in:Journal of prosthodontics Vol. 27; no. 7; pp. 665 - 669
Main Authors: Naito, Yoshihito, Yumoto, Hiromichi, Kumar HS, Kiran, Matsuo, Takashi, Hirota, Katsuhiko, Miyake, Yoichiro, Nagao, Kan, Tomotake, Yoritoki, Jimbo, Ryo, Ichikawa, Tetsuo
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-08-2018
Subjects:
Antifungal activity
Antifungal Agents - pharmacology
Biofilms
Biofilms - drug effects
Candida albicans
Candida albicans - drug effects
Candidiasis
Cell Proliferation - drug effects
Dentistry
Fluidity
In Vitro Techniques
Juncus powder
Magnoliopsida
Mechanical properties
mechanical property
Microscopy, Electron, Scanning
Optical density
Plant Extracts - pharmacology
Scanning electron microscopy
Stomatitis
Tissue conditioner
Tissue Conditioning, Dental - methods
Tissue culture
Candida albicans
Juncus powder
Tissue conditioner
mechanical property
antifungal activity
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Summary:Purpose To evaluate the antifungal activity and mechanical properties of a novel antifungal tissue conditioner containing Juncus powder. Materials and Methods Juncus powder was mixed with GC tissue conditioner at concentrations of 2.5%, 5.0%, and 10.0% by mass. The cylindrical specimens of Juncus‐mixed tissue conditioner (dimensions: 10 mm in diameter and 2 and 6 mm in height for antimicrobial and mechanical tests, respectively) were prepared. The specimens placed on the bottom of the 24‐well tissue culture plate were cultured with Candida albicans CAD1 for 2 and 4 days. The proliferation of the C. albicans in the wells was determined by measuring the optical density of fungal culture, and the surface of the specimens were also observed by scanning electron microscopy (SEM). To assess the mechanical properties of the specimens, the fluidity and hardness of Juncus‐mixed tissue conditioner were measured using the methods certified according to ISO 10139‐1. Results Juncus‐mixed tissue conditioner significantly exhibited growth inhibitory effect in a Juncus concentration‐dependent manner after both 2‐ and 4‐ day cultures. SEM observation showed that the amount of C. albicans on Juncus‐mixed specimens drastically decreased, and biofilm formation was markedly inhibited. Moreover, both mechanical properties were found to be within the ranges regulated and specified by ISO. Conclusion These findings demonstrated that the tissue conditioner including Juncus powder has a significant growth inhibitory effect against C. albicans, and it is suggested that the application of Juncus‐mixed tissue conditioner may prevent denture stomatitis and oral candidiasis in denture wearers.
Bibliography:The authors deny any conflicts of interests
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ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1059-941X
1532-849X
1532-849X
DOI:10.1111/jopr.12546