Search Results - "Kumar, P.K.R"
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Isolation of RNA Aptamers Specific to the NS3 Protein of Hepatitis C Virus from a Pool of Completely Random RNA
Published in Virology (New York, N.Y.) (27-10-1997)“…Hepatitis C virus (HCV) is a single-stranded RNA virus and its genome is translated into a single large polyprotein. The viral-encoded NS3 protein possesses…”
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Influence of medium- and long-range interactions in different folding types of globular proteins
Published in Biophysical chemistry (16-10-2002)“…Recognition of protein fold from amino acid sequence is a challenging task. The structure and stability of proteins from different fold are mainly dictated by…”
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3
Immobilization of genetically engineered cells: a new strategy for higher stability
Published in Journal of biotechnology (01-06-1990)“…The r-DNA clones improve the bioprocess and provide better economics, if and when properly developed. In recent times, many approaches were made to improve the…”
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Plasmid instabilities of single and three-plasmid systems in Escherichia coli during continuous cultivation
Published in Journal of biotechnology (1992)“…Plasmid instabilities in E. coli JM103 carrying three plasmids (pRK248cI, pMTC48, pEcoR4) and a single plasmid system (pTG206) for the production of fusion…”
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Aptamer-Derived Nucleic Acid Oligos: Applications to Develop Nucleic Acid Chips to Analyze Proteins and Small Ligands
Published in Analytical chemistry (Washington) (01-09-2005)“…The specificity and affinity of aptamers for their cognate ligands are comparable to those of antibodies for antigens. To use aptamers effectively in…”
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Immobilized growing cells of Gibberella fujikuroi P-3 for production of gibberellic acid and pigment in batch and semi-continuous cultures
Published in Applied microbiology and biotechnology (01-07-1988)“…The performance of immobilized growing cells of Gibberella fujikuroi P-3 was affected by the immobilization agent used, nature and age of cells, mycelial cell…”
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Structure in the local environment of Zn 2+ ion in the anti-termination protein of Bacillus subtilis
Published in Acta crystallographica. Section A, Foundations of crystallography (23-08-2008)“…Abstract only…”
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Crystallization and preliminary X-ray diffraction studies of the metal-ion-mediated ternary complex of the HutP protein with l-histidine and its cognate RNA
Published in Biochimica et biophysica acta (01-10-2004)“…HutP is an RNA-binding protein that regulates the expression of the Bacillus subtilis hut operon by binding to cis-acting regulatory sequences within hut mRNA,…”
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In vitro selection of RNA aptamers against cellular and abnormal isoform of mouse prion protein
Published in Nucleic Acids Symposium Series (01-09-2005)“…Prion disease is caused by conformational change of normal cellular type of prion protein (PrPc) folding into abnormal type (PrPsc). We succeeded to isolate…”
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Crystal structure of a decamer RNA r(ggcggucgcu)2 with terminal and tandem G·U wobble base-pairs
Published in Nucleic Acids Symposium Series (01-09-2003)“…The data set of the crystal structure of a self-complementary RNA duplex r(ggcggucgcu)2 with terminal and tandem G·U wobble base-pairs has been collected to…”
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Crystallization and preliminary X-ray diffraction studies of HutP protein: an RNA-binding protein that regulates the transcription of hut operon in Bacillus subtilis
Published in Journal of structural biology (01-06-2002)“…HutP is an RNA-binding protein and regulates the expression of the histidine utilization ( hut) operon in Bacillus subtilis by binding to cis-acting regulatory…”
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12
Expression of highly active recombinant NS3 protease domain of hepatitis C virus in E. coli
Published in FEBS letters (03-02-1997)“…The serine protease domain of HCV comprising amino acids 1027–1218 (ΔNS3) was expressed in E. coli with a His tag at its N-terminal end. The protease was…”
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Identification of phosphate oxygens that are important for self-cleavage activity of the HDV ribozyme by phosphorothioate substitution interference analysis
Published in Nucleic acids research (11-09-1994)“…A phosphorothioate substitution interference assay was used to investigate the role of the pro-Rp oxygens of phosphate groups in the self-cleavage reaction of…”
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14
Strategies for improving plasmid stability in genetically modified bacteria in bioreactors
Published in Trends in biotechnology (Regular ed.) (01-08-1991)“…Exploitation of recombinant organisms for the large-scale, commercial production of foreign proteins is often hampered by the problem of plasmid instability. A…”
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Random mutations to evaluate the role of bases at two important single-stranded regions of genomic HDV ribozyme
Published in Nucleic acids research (11-08-1992)“…In elucidating function of two Important single-stranded regions [SSrA (726–731 nt) and SSrB (762–766 nt)] derived mainly from three secondary structure models…”
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Systematic substitution of individual bases in two important single-stranded regions of the HDV ribozyme for evaluation of the role of specific bases
Published in FEBS letters (1993)“…To elucidate the role of specific bases in the self-cleavage activity of the human hepatitis delta virus (HDV) ribozyme, systematic substitutions of individual…”
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Solid state fermentation: physical and nutritional factors influencing gibberellic acid production
Published in Applied microbiology and biotechnology (01-11-1990)“…The production of gibberellic acid (GA3) was found to be influenced by physical and nutritional factors. The nutritional factors studied were the urea nitrogen…”
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Formation of acetic acid from cellulosic substrates by Fusarium oxysporum
Published in Applied microbiology and biotechnology (01-02-1991)“…Four strains of Fusarium oxysporum and a strain of Monilia brunnae were screened for their ability to convert cellulosic substrates into ethanol/acetic acid…”
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Deletion of internal sequence on the HDV-ribozyme: elucidation of functionally important single-stranded loop regions
Published in Nucleic acids research (25-02-1992)“…In elucidating functionally important single-stranded loop regions derived mainly from three models in genomic hepatitis delta virus (HDV) ribozyme possessing…”
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Fusarium oxysporum: status in bioethanol production
Published in Critical reviews in biotechnology (1991)“…Fermentation of lignocellulosic materials to ethanol and other solvents provides an alternative way of treating wastes and producing chemical feedstocks and…”
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