Search Results - "Kumagai, Izumi"

Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries by Nakazawa, Hikaru, Okada, Izumi, Ito, Tomoyuki, Ishigaki, Yuri, Kumagai, Izumi, Umetsu, Mitsuo

“…Cellulase selectively recognizes cellulose surfaces and cleaves their β-1,4-glycosidic bonds. Combining hydrolysis using cellulase and fermentation can produce…”
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Engineering the hinge region of human IgG1 Fc-fused bispecific antibodies to improve fragmentation resistance by Suzuki, Saori, Annaka, Hiroaki, Konno, Shota, Kumagai, Izumi, Asano, Ryutaro

“…Fc domain fusion can improve the therapeutic effects of relatively small biological molecules such as peptides, cytokines, and antibody fragments. Fc fusion…”
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Construction of IgG–Fab2 bispecific antibody via intein-mediated protein trans-splicing reaction by Yamada, Risa, Nakahara, Ishin, Kumagai, Izumi, Asano, Ryutaro, Nakanishi, Takeshi, Makabe, Koki

“…A bispecific antibody (bsAb) is a class of engineered antibody molecules that simultaneously binds to two different antigens by having two kinds of…”
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Functional Domain Order of an Anti-EGFR × Anti-CD16 Bispecific Diabody Involving NK Cell Activation by Kuwahara, Atsushi, Nagai, Keisuke, Nakanishi, Takeshi, Kumagai, Izumi, Asano, Ryutaro

“…Bispecific antibodies (bsAbs) have emerged as promising therapeutics. A bispecific diabody (bsDb) is a small bsAb consisting of two distinct chimeric…”
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Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope by Makabe, Koki, Yokoyama, Takeshi, Uehara, Shiro, Uchikubo-Kamo, Tomomi, Shirouzu, Mikako, Kimura, Kouki, Tsumoto, Kouhei, Asano, Ryutaro, Tanaka, Yoshikazu, Kumagai, Izumi

“…Antibodies have been widely used for cancer therapy owing to their ability to distinguish cancer cells by recognizing cancer-specific antigens. Epidermal…”
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Construction of a circularly connected VHH bispecific antibody (cyclobody) for the desirable positioning of antigen-binding sites by Hemmi, Saki, Asano, Ryutaro, Kimura, Kouki, Umetsu, Mitsuo, Nakanishi, Takeshi, Kumagai, Izumi, Makabe, Koki

“…A bispecific antibody (bsAb) is an emerging class of next-generation biological therapeutics. BsAbs are engineered antibodies possessing dual antigen-binding…”
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Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats by Asano, Ryutaro, Hosokawa, Katsuhiro, Taki, Shintaro, Konno, Shota, Shimomura, Ippei, Ogata, Hiromi, Okada, Mai, Arai, Kyoko, Onitsuka, Masayoshi, Omasa, Takeshi, Nakanishi, Takeshi, Umetsu, Mitsuo, Kumagai, Izumi

“…Designing non-natural antibody formats is a practical method for developing highly functional next-generation antibody drugs, particularly for improving the…”
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Structural Basis for Recognition of the Nonclassical MHC Molecule HLA-G by the Leukocyte Ig-Like Receptor B2 (LILRB2/LIR2/ILT4/CD85d) by Shiroishi, Mitsunori, Kuroki, Kimiko, Rasubala, Linda, Tsumoto, Kouhei, Kumagai, Izumi, Kurimoto, Eiji, Kato, Koichi, Kohda, Daisuke, Maenaka, Katsumi

“…HLA-G is a nonclassical MHC class I (MHCI) molecule that can suppress a wide range of immune responses in the maternal-fetal interface. The human inhibitory…”
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Complementary Design for Pairing between Two Types of Nanoparticles Mediated by a Bispecific Antibody: Bottom-Up Formation of Porous Materials from Nanoparticles by Niide, Teppei, Manabe, Noriyoshi, Nakazawa, Hikaru, Akagi, Kazuto, Hattori, Takamitsu, Kumagai, Izumi, Umetsu, Mitsuo

“…Recent advances in biotechnology have enabled the generation of antibodies with high affinity for the surfaces of specific inorganic materials. Herein, we…”
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Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units by Yoshida, Jyunna, Kato, Yuki, Isogawa, Ai, Tanaka, Yoshikazu, Kumagai, Izumi, Asano, Ryutaro, Nakanishi, Takeshi, Makabe, Koki

“…During the production of bispecific antibodies (bsAbs), nonspecific pairing results in low yields of target bsAb molecules, an issue known as the "mispairing…”
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Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical Microscopy by Takahashi, Yasufumi, Miyamoto, Takeshi, Shiku, Hitoshi, Asano, Ryutaro, Yasukawa, Tomoyuki, Kumagai, Izumi, Matsue, Tomokazu

“…A membrane protein on the surface of a single living mammalian cell was imaged by scanning electrochemical microscopy (SECM). The epidermal growth factor…”
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Effective Treatment of an Orthotopic Xenograft Model of Human Glioblastoma Using an EGFR-retargeted Oncolytic Herpes Simplex Virus by Uchida, Hiroaki, Marzulli, Marco, Nakano, Kenji, Goins, William F, Chan, Janet, Hong, Chang-Sook, Mazzacurati, Lucia, Yoo, Ji Young, Haseley, Amy, Nakashima, Hiroshi, Baek, Hyunjung, Kwon, Heechung, Kumagai, Izumi, Kuroki, Masahide, Kaur, Balveen, Chiocca, E Antonio, Grandi, Paola, Cohen, Justus B, Glorioso, Joseph C

“…Glioblastoma multiforme (GBM) remains an untreatable human brain malignancy. Despite promising preclinical studies using oncolytic herpes simplex virus (oHSV)…”
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Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold by Saito, Ryota, Saito, Yutaro, Nakazawa, Hikaru, Hattori, Takamitsu, Kumagai, Izumi, Umetsu, Mitsuo, Makabe, Koki

“…Abstract Biomolecules which recognize inorganic materials and metal surfaces gain much attention for creating new type of nanomaterials and sensors. 4F2, a…”
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Identification of Indium Tin Oxide Nanoparticle-Binding Peptides via Phage Display and Biopanning Under Various Buffer Conditions by Nakazawa, Hikaru, Umetsu, Mitsuo, Hirose, Tatsuya, Hattori, Takamitsu, Kumagai, Izumi

“…By recent advances in phage-display approaches, many oligopeptides exhibiting binding affinities for metal oxides have been identified. Indium tin oxide is one…”
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Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach by Ueda, Asami, Umetsu, Mitsuo, Nakanishi, Takeshi, Hashikami, Kentaro, Nakazawa, Hikaru, Hattori, Shuhei, Asano, Ryutaro, Kumagai, Izumi

“…Antibodies are composed of structurally and functionally independent domains that can be used as building blocks to construct different types of chimeric…”
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High-throughput cytotoxicity and antigen-binding assay for screening small bispecific antibodies without purification by Sugiyama, Aruto, Umetsu, Mitsuo, Nakazawa, Hikaru, Niide, Teppei, Asano, Ryutaro, Hattori, Takamitsu, Kumagai, Izumi

“…The cytotoxicity of T cell-recruiting antibodies with their potential to damage late-stage tumor masses is critically dependent on their structural and…”
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A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity by Sugiyama, Aruto, Umetsu, Mitsuo, Nakazawa, Hikaru, Niide, Teppei, Onodera, Tomoko, Hosokawa, Katsuhiro, Hattori, Shuhei, Asano, Ryutaro, Kumagai, Izumi

“…Small bispecific antibodies that induce T-cell–mediated cytotoxicity have the potential to damage late-stage tumor masses to a clinically relevant degree, but…”
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Association behavior and control of the quality of cancer therapeutic bispecific diabodies expressed in Escherichia coli by Nakazawa, Hikaru, Onodera-Sugano, Tomoko, Sugiyama, Aruto, Tanaka, Yoshikazu, Hattori, Takamitsu, Niide, Teppei, Ogata, Hiromi, Asano, Ryutaro, Kumagai, Izumi, Umetsu, Mitsuo

“…•Nearly half the LH diabody fraction and all the HL fraction were in inactive form.•Diabody fraction components changed dynamically with time.•The association…”
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A High-Affinity Gold-Binding Camel Antibody: Antibody Engineering for One-Pot Functionalization of Gold Nanoparticles as Biointerface Molecules by Hattori, Takamitsu, Umetsu, Mitsuo, Nakanishi, Takeshi, Sawai, Satoko, Kikuchi, Shinsuke, Asano, Ryutaro, Kumagai, Izumi

“…Antibodies, with their high affinity and specificity, are widely utilized in the field of protein engineering, medicinal chemistry, and nanotechnology…”
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Practical considerations in refolding proteins from inclusion bodies by Tsumoto, Kouhei, Ejima, Daisuke, Kumagai, Izumi, Arakawa, Tsutomu

“…Refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the…”
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