Search Results - "Kulomaa, M"

Genetically engineered avidins and streptavidins by Laitinen, O. H, Hytönen, V. P, Nordlund, H. R, Kulomaa, M. S

“…Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification…”
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Enumeration of Methanotrophic Bacteria in the Cover Soil of an Aged Municipal Landfill by Kallistova, A. Yu, Kevbrina, M. V, Nekrasova, V. K, Shnyrev, N. A, Einola, J-K. M, Kulomaa, M. S, Rintala, J. A, Nozhevnikova, A. N

“…The enumeration of methanotrophic bacteria in the cover soil of an aged municipal landfill was carried out using (1) fluorescent in situ hybridization (FISH)…”
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Latex allergy: the sum quantity of four major allergens shows the allergenic potential of medical gloves by Reinikka-Railo, H, Kautiainen, H, Alenius, H, Kalkkinen, N, Kulomaa, M, Reunala, T, Turjanmaa, K

“…Assessment of allergenic potential of medical devices made of natural rubber latex (NRL) requires the measurement of concentrations of specific allergenic…”
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Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery by AIRENNE, K. J, HILTUNEN, M. O, TURUNEN, M. P, TURUNEN, A-M, LAITINEN, O. H, KULOMAA, M. S, YLÄ-HERTTUALA, S

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Targeting of biotinylated compounds to its target tissue using a low-density lipoprotein receptor-avidin fusion protein by LEHTOLAINEN, P, WIRTH, T, HORTON, M, KULOMAA, M. S, YLÄ-HERTTUALA, S, TASKINEN, A. K, LEHENKARI, P, LEPPÄNEN, O, LAPPALAINEN, M, PULKKANEN, K, MARTTILA, A, MARJOMÄKI, V, AIRENNE, K. J

“…The very high binding affinity of avidin to biotin is one of the highest to occur in nature. We constructed a fusion protein composed of avidin and the…”
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Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning by Lehtonen, Soili I., Taskinen, Barbara, Ojala, Elina, Kukkurainen, Sampo, Rahikainen, Rolle, Riihimäki, Tiina A., Laitinen, Olli H., Kulomaa, Markku S., Hytönen, Vesa P.

“…Efficient and robust subcloning is essential for the construction of high-diversity DNA libraries in the field of directed evolution. We have developed a more…”
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Isolation and Characterization of Novosphingobium sp. Strain MT1, a Dominant Polychlorophenol-Degrading Strain in a Groundwater Bioremediation System by TIIROLA, Marja A, MÄNNISTÖ, Minna K, PUHAKKA, Jaakko A, KULOMAA, Markku S

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Diversity of chlorophenol-degrading bacteria isolated from contaminated boreal groundwater by MÄNNISTÖ, M. K, TIIROLA, M. A, SALKINOJA-SALONEN, M. S, KULOMAA, M. S, PUHAKKA, J. A

“…Chlorophenol-degrading bacteria from a long-term polluted groundwater aquifer were characterized. All isolates degraded 2,4,6-trichlorophenol and…”
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Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery by Airenne, K J, Hiltunen, M O, Turunen, M P, Turunen, A M, Laitinen, O H, Kulomaa, M S, Ylä-Herttuala, S

“…Recombinant Autographa californica multiple nuclear polyhedrosis viruses (AcMNPV) have recently been shown to transduce mammalian cells in vitro. Since…”
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Characterization and chromosomal localization of the chicken avidin gene family by Ahlroth, M K, Kola, E H, Kulomaa, M S, Ewald, D, Masabanda, J, Sazanov, A, Fries, R

“…Chicken avidin is a biotin‐binding protein expressed under inflammation in several chicken tissues and in the oviduct after progesterone induction. The gene…”
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N-terminal hevein-like domains (4.3 kDa) but not 31 kDa endochitinases are responsible for IgE-mediated in vitro and in vivo reactions in latex-fruit syndrome by Karisola, P., Kotovuori, A., Poikonen, S., Niskanen, E., Kalkkinen, N., Turjanmaa, K., Palosuo, T., Reunala, T., Alenius, H., Kulomaa, M.

“…IgE-mediated clinical reactions in latex-fruit syndrome seem to be evoked by 4.3 kDa HLD molecules alone but not when linked to 31 kDa endochitinases. [...]a…”
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Microbial diversity in a thermophilic aerobic biofilm process: analysis by length heterogeneity PCR (LH-PCR) by Tiirola, Marja A., Suvilampi, Juhani E., Kulomaa, Markku S., Rintala, Jukka A.

“…A two-stage pilot-scale thermophilic aerobic suspended carrier biofilm process (SCBP) was set up for the on-site treatment of pulp and paper mill whitewater…”
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Recombinant NeutraLite Avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties by Marttila, Ari T., Laitinen, Olli H., Airenne, Kari J., Kulik, Tikva, Bayer, Edward A., Wilchek, Meir, Kulomaa, Markku S.

“…A recombinant non-glycosylated and acidic form of avidin was designed and expressed in soluble form in baculovirus-infected insect cells. The mutations were…”
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Hevein-like domain IV in wheat germ agglutinin (WGA) is responsible for the majority of IgE cross-reactivity between WGA and hevein (Hev b 6.02) by Karisola, P., Alenius, H., Turjanmaa, K., Reunala, T., Kalkkinen, N., Palosuo, T., Kulomaa, M.

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Recombinant allergen-based ELISA for the simultaneous detection of IgE to clinically relevant latex allergens by Palosuo, T., Karkkainen, T., Frisk, K., Turjanmaa, K., Alenius, H., Kulomaa, M., Kalkkinen, N., Reunala, T.

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Biotin induces tetramerization of a recombinant monomeric avidin. A model for protein-protein interactions by Laitinen, O H, Marttila, A T, Airenne, K J, Kulik, T, Livnah, O, Bayer, E A, Wilchek, M, Kulomaa, M S

“…Chicken avidin, a homotetramer that binds four molecules of biotin was converted to a monomeric form by successive mutations of interface residues to alanine…”
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Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain by Laitinen, Olli H., Airenne, Kari J., Marttila, Ari T., Kulik, Tikva, Porkka, Eevaleena, Bayer, Edward A., Wilchek, Meir, Kulomaa, Markku S.

“…Sea urchin fibropellins are epidermal growth factor homologues that harbor a C-terminal domain, similar in sequence to hen egg-white avidin and bacterial…”
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Production and Characterization of the Recombinant Sphingomonas chlorophenolica Pentachlorophenol 4-Monooxygenase by Wang, Hong, Tiirola, Marja A., Puhakka, Jaakko A., Kulomaa, Markku S.

“…Pentachlorophenol 4-monooxygenase (PCP4MO) from Sphingomonas chlorophenolica is a flavoprotein that hydroxylates PCP in the presence of NADPH and oxygen. In…”
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Copy-Number Fluctuation by Unequal Crossing-Over in the Chicken Avidin Gene Family by Ahlroth, Mervi K., Ahlroth, Petri, Kulomaa, Markku S.

“…The chicken avidin gene (AVD) forms a closely clustered gene family together with several avidin-related genes (AVRs). In this study, we used fluorescence in…”
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Sequence Features and Evolutionary Mechanisms in the Chicken Avidin Gene Family by Ahlroth, Mervi K., Grapputo, Alessandro, Laitinen, Olli H., Kulomaa, Markku S.

“…The chicken avidin gene family comprises the avidin gene (avd) and several homologous avidin-related genes (avrs). The sequences of the avr genes are nearly…”
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