Different Types of Urinary Steroid Profiling Obtained by High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry in Patients with Adrenocortical Carcinoma

Different Types of Urinary Steroid Profiling Obtained by High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry in Patients with Adrenocortical Carcinoma

Urinary steroid profiling (USP) was studied using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) methods in 108 patients with adrenocortical adenoma (ACA) and in 31 patients with adrenocortical carcinoma (ACC). Thirteen ACC and Cushing’s syndrome (ACC-...

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Bibliographic Details
Published in:Hormones & cancer Vol. 7; no. 5-6; pp. 327 - 335
Main Authors: Velikanova, L. I., Shafigullina, Z. R., Lisitsin, A. A., Vorokhobina, N. V., Grigoryan, K., Kukhianidze, E. A., Strelnikova, E. G., Krivokhizhina, N. S., Krasnov, L. M., Fedorov, E. A., Sablin, I. V., Moskvin, A. L., Bessonova, E. A.
Format: Journal Article
Language:English
Published: New York Springer US 01-12-2016
Subjects:
Adrenal Cortex Neoplasms - diagnosis
Adrenal Cortex Neoplasms - urine
Adrenocortical Adenoma - diagnosis
Adrenocortical Adenoma - urine
Adrenocortical Carcinoma - diagnosis
Adrenocortical Carcinoma - urine
Adult
Cell Biology
Chromatography, High Pressure Liquid - methods
Cortodoxone - analogs & derivatives
Cortodoxone - urine
Cushing Syndrome - urine
Dehydroepiandrosterone - urine
Diagnosis, Differential
Endocrinology
Female
Gas Chromatography-Mass Spectrometry - methods
Humans
Internal Medicine
Male
Medicine
Medicine & Public Health
Microbiology
Middle Aged
Oncology
Original Paper
Steroid 11-beta-Hydroxylase - metabolism
Steroid 21-Hydroxylase - metabolism
Steroids - urine
Systems Biology
Young Adult
Adrenocortical Carcinoma
Primary Hyperaldosteronism
Adrenal Tumor
Adrenal Mass
Congenital Adrenal Hyperplasia
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Summary:Urinary steroid profiling (USP) was studied using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) methods in 108 patients with adrenocortical adenoma (ACA) and in 31 patients with adrenocortical carcinoma (ACC). Thirteen ACC and Cushing’s syndrome (ACC-CS) patients had two types of USP as well as 18 ACC patients without hypercortisolism. These four types differed by androgen and glucocorticoid secretion of the adrenal cortex. Fifteen main ACC features were observed by GC-MS. Urinary excretion of dehydroepiandrosterone (DHEA) was increased in 67.7 % of ACC patients and tetrahydro-11-deoxycortisol (THS) in 74.2 %. By combination of the following parameters: THS >900 μg/24 h and/or DHEA >1500 μg/24 h with ratios of 3α,16,20-pregnentriol/3β,16,20-pregnentriol (3α,16,20dP3/3β,16,20dP3) less than 6.0 and 3α,17,20dP3/3β,17,20dP3 less than 9.0 and the detection of “non-classical” 5-en-pregnens, not found in ACA and healthy persons, 100 % sensitivity and specificity of ACC and ACA differential diagnosis were achieved. Features of 21-hydroxylase and 11β-hydroxylase deficiency were observed by GC-MS in 32.2 and 61.3 % of the ACC patients, respectively. Additional features for ACC-CS diagnostic were increased urinary excretion of 6β-hydroxycortisol, 18-hydroxycorticosterone, the sum (UFF + UFE) obtained by HPLC, tetrahydrocorticosterone, and the sum (THF + THE + allo-THF) obtained by GC-MS.
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ISSN:1868-8497
1868-8500
DOI:10.1007/s12672-016-0267-0