DNA immunization induces antibody and cytotoxic T cell responses to hepatitis B core antigen in H-2b mice

The serum Ab response and the class I-restricted CTL response of C57BL/6 (H-2b) mice to hepatitis B (pre)core Ag (HBcAg, HBeAg) was studied. Injection of HBcAg particles without adjuvants into mice efficiently primed serum Ab responses but not CTL response. We constructed the expression plasmids pCM...

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Bibliographic Details
Published in:	The Journal of immunology (1950) Vol. 156; no. 10; pp. 3687 - 3695
Main Authors:	Kuhober, A, Pudollek, HP, Reifenberg, K, Chisari, FV, Schlicht, HJ, Reimann, J, Schirmbeck, R
Format:	Journal Article
Language:	English
Published:	United States Am Assoc Immnol 15-05-1996
Subjects:	AIDS/HIV Amino Acid Sequence Animals CD8-Positive T-Lymphocytes - classification DNA, Viral - administration & dosage DNA, Viral - immunology Epitopes - chemistry Epitopes - genetics Epitopes - immunology Female H-2 Antigens - genetics H-2 Antigens - immunology Hepatitis B Antibodies - biosynthesis Hepatitis B Core Antigens - genetics Hepatitis B Core Antigens - immunology Hepatitis B Vaccines - immunology Hepatitis B virus - immunology Male Mice Mice, Inbred C57BL Molecular Sequence Data Plasmids - immunology T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Cytotoxic - virology Transfection - immunology Vaccines, Synthetic - immunology
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Summary:	The serum Ab response and the class I-restricted CTL response of C57BL/6 (H-2b) mice to hepatitis B (pre)core Ag (HBcAg, HBeAg) was studied. Injection of HBcAg particles without adjuvants into mice efficiently primed serum Ab responses but not CTL response. We constructed the expression plasmids pCMV-1/c and pCMV-1/e in which expression of HBcAg or HBeAg was driven by cytomegalovirus immediate early region promoter sequences. Stable murine RBL5/C transfectant lines expressing HBcAg were established. Intramuscular DNA immunization with plasmid pCMV-1/c (encoding intracellularly expressed core Ag) or pCMV-1/e (encoding secreted precore Ag) efficiently primed specific serum Ab responses and CTL responses. The CTL response elicited in this system was mediated by CD4-CD8+ effector cells primed in vivo. The CTL recognized the HBcAg93-100 8-mer peptide MGLKFRQL in the context of Kb. Hence, DNA immunization with HBcAg/HBeAg-expressing plasmids, but not immunization with exogenous HBcAg particles, elicits a class I-restricted CTL response of defined epitope/restriction specificity in H-2b mice.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1767 1550-6606
DOI:	10.4049/jimmunol.156.10.3687