Search Results - "Kuehner, Jason"
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Unravelling the means to an end: RNA polymerase II transcription termination
Published in Nature reviews. Molecular cell biology (01-05-2011)“…Key Points RNA polymerase II (Pol II) transcribes all eukaryotic protein-coding genes and most non-coding RNA genes. The final step of transcription is…”
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Regulation of a Eukaryotic Gene by GTP-Dependent Start Site Selection and Transcription Attenuation
Published in Molecular cell (25-07-2008)“…Guanine nucleotide negatively regulates yeast inosine monophosphate dehydrogenase (IMPDH) mRNA synthesis by an unknown mechanism. IMPDH catalyzes the first…”
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Regulation of a Eukaryotic Gene by GTP-Dependent Start Site Selection and Transcription Attenuation
Published in Molecular cell (02-05-2019)Get full text
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Stimulation of RNA Polymerase II ubiquitination and degradation by yeast mRNA 3′-end processing factors is a conserved DNA damage response in eukaryotes
Published in DNA repair (01-09-2017)“…[Display omitted] •Yeast mRNA 3′-end processing factors CFIA and CPF promote tolerance to UV damage.•CFIA/CPF contribution to DNA integrity is not limited to…”
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Genome-Wide Distribution of Yeast RNA Polymerase II and Its Control by Sen1 Helicase
Published in Molecular cell (08-12-2006)“…Functional engagement of RNA polymerase II (Pol II) with eukaryotic chromosomes is a fundamental and highly regulated biological process. Here we present a…”
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Mutations in yeast Pcf11, a conserved protein essential for mRNA 3′ end processing and transcription termination, elicit the Environmental Stress Response
Published in Genetics (Austin) (07-02-2024)“…Abstract The Pcf11 protein is an essential subunit of the large complex that cleaves and polyadenylates eukaryotic mRNA precursor. It has also been…”
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RNA polymerase II transcription attenuation at the yeast DNA repair gene DEF1 is biologically significant and dependent on the Hrp1 RNA-recognition motif
Published in G3 : genes - genomes - genetics (12-01-2023)“…Abstract Premature transcription termination (i.e. attenuation) is a potent gene regulatory mechanism that represses mRNA synthesis. Attenuation of RNA…”
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DNA damage induces targeted, genome-wide variation of poly(A) sites in budding yeast
Published in Genome research (01-10-2013)“…Systemic response to DNA damage and other stresses is a complex process that includes changes in the regulation and activity of nearly all stages of gene…”
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Quantitative Analysis of in Vivo Initiator Selection by Yeast RNA Polymerase II Supports a Scanning Model
Published in The Journal of biological chemistry (19-05-2006)“…Initiation of transcription by RNA polymerase II (RNAP II) on Saccharomyces cerevisiae messenger RNA (mRNA) genes typically occurs at multiple sites 40–120 bp…”
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RNA Polymerase II Transcription Attenuation at the Yeast DNA Repair Gene, DEF1 , Involves Sen1-Dependent and Polyadenylation Site-Dependent Termination
Published in G3 : genes - genomes - genetics (01-06-2018)“…Termination of RNA Polymerase II (Pol II) activity serves a vital cellular role by separating ubiquitous transcription units and influencing RNA fate and…”
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Reconstitution of CF IA from Overexpressed Subunits Reveals Stoichiometry and Provides Insights into Molecular Topology
Published in Biochemistry (Easton) (29-11-2011)“…Yeast cleavage factor I (CF I) is an essential complex of five proteins that binds signal sequences at the 3′ end of yeast mRNA. CF I is required for correct…”
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Money, Sex, and Drugs: A Case Study to Teach the Genetics of Antibiotic Resistance
Published in CBE life sciences education (01-01-2008)“…The goal of the work reported here was to help students expand their understanding of antibiotic resistance, the Central Dogma, and evolution. We developed a…”
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A simple, robust, broadly applicable insertion mutagenesis method to create random fluorescent protein: target protein fusions
Published in G3 : genes - genomes - genetics (07-05-2024)“…A simple, broadly applicable method was developed using an in vitro transposition reaction followed by transformation into Escherichia coli and screening…”
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Mechanism and regulation of yeast RNA polymerase II transcription initiation and termination
Published 01-01-2008“…RNA Polymerase II (Pol II) transcribes most eukaryotic genes and is subject to extensive regulatory control. Initiation of Pol II transcription in the yeast…”
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