In vitro antibacterial activity of ciprofloxacin loaded chitosan microparticles and their effects on human lung epithelial cells

In vitro antibacterial activity of ciprofloxacin loaded chitosan microparticles and their effects on human lung epithelial cells

[Display omitted] Chitosan (CS), due to its inherent mucoadhesive property and biofilm penetration ability, can be considered as very potent vehicle for local drug delivery to the lungs. This study reports on the preparation and in vitro antibacterial activity and cytotoxicity determination of cipro...

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Bibliographic Details
Published in:International journal of pharmaceutics Vol. 569; p. 118578
Main Authors: Kucukoglu, Vildan, Uzuner, Huseyin, Kenar, Halime, Karadenizli, Aynur
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 05-10-2019
Subjects:
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - chemistry
Antibacterial
Biocompatibility
Cell Line
Cell Survival - drug effects
Chitosan - administration & dosage
Chitosan - chemistry
Chitosan microparticles
Ciprofloxacin
Ciprofloxacin - administration & dosage
Ciprofloxacin - chemistry
Drug Liberation
Epithelial Cells - drug effects
Escherichia coli - drug effects
Humans
Lung - cytology
Microbial Sensitivity Tests
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Pseudomonas aeruginosa - drug effects
Pulmonary drug delivery
Staphylococcus aureus - drug effects
Cipro-CS
MIC
Chitosan microparticles
Pulmonary drug delivery
DLS
Antibacterial
MBC
CS
TPP
Biocompatibility
SEM
Ciprofloxacin
BEAS-2B
TEM
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Summary:[Display omitted] Chitosan (CS), due to its inherent mucoadhesive property and biofilm penetration ability, can be considered as very potent vehicle for local drug delivery to the lungs. This study reports on the preparation and in vitro antibacterial activity and cytotoxicity determination of ciprofloxacin loaded chitosan (Cipro-CS) microparticles with size in the range of 0.1–1 µm, which may provide advantages of lower nanotoxicity and lower local clearance. Cipro-CS microparticles were prepared by ionic gelation method and their size, zeta potential and drug release pattern determined. The antibacterial activities of CS and Cipro-CS microparticles against pneumonia causing agents, namely Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, were evaluated by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The biocompatibility of the microparticles was tested in the human lung epithelial cell (BEAS-2B) culture, and microparticle association with the bacteria and epithelial cells was evaluated by transmission electron microscopy. Only the Cipro-CS microparticles, but not the CS microparticles, inhibited bacterial growth at concentrations not significantly cytotoxic to BEAS-2B cells. The Cipro-CS microparticles were able to damage the cell wall and membrane of the bacteria, and the ones ≤200 nm in size were internalized by both the BEAS-2B cells and the microorganisms.
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content type line 23
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2019.118578