Prediction of peptide retention volumes in the gradient reversed phase HPLC

A method of computation of retention volumes of linear peptides of known composition that contain no more than 25 amino acids in gradient reversed phase HPLC was developed. The method is suitable for various acetonitrile gradient profiles. The calculations were carried out on the basis of a statisti...

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Bibliographic Details
Published in:	Bioorganicheskaia khimiia Vol. 34; no. 2; p. 171
Main Authors:	Azarova, I N, Kuchkina, A Iu, Baram, G I, Gol'dberg, E L
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-03-2008
Subjects:	Acetonitriles Amino Acid Sequence Chromatography, High Pressure Liquid Models, Chemical Molecular Sequence Data Oligopeptides - chemistry Peptides - chemistry Solvents
Online Access:	Get more information
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Summary:	A method of computation of retention volumes of linear peptides of known composition that contain no more than 25 amino acids in gradient reversed phase HPLC was developed. The method is suitable for various acetonitrile gradient profiles. The calculations were carried out on the basis of a statistical model, the parameters of which were experimental dependences of the retention of individual amino acids on acetonitrile concentration. The method developed was used to predict the chromatographic behavior of 34 peptides in four different acetonitrile gradients. The correlation coefficients between the predicted and experimental retention volumes were more than 0.9, and the average relative error of prediction was less than 15%. The English version of the paper: Russiatn .lournal of Bioorganic Chemistry, 2008, vol. 34, no. 2; see also http://www.maik.ru.
ISSN:	0132-3423