Search Results - "Kuchenreuther, Jon M."

A Radical Intermediate in Tyrosine Scission to the CO and CN⁻ Ligands of FeFe Hydrogenase by Kuchenreuther, Jon M., Myers, William K., Stich, Troy A., George, Simon J., NejatyJahromy, Yaser, Swartz, James R., Britt, R. David

“…The radical S-adenosylmethionine (SAM) enzyme HydG lyses free L-tyrosine to produce CO and CN⁻ for the assembly of the catalytic H cluster of FeFe hydrogenase…”
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High-yield expression of heterologous [FeFe] hydrogenases in Escherichia coli by Kuchenreuther, Jon M, Grady-Smith, Celestine S, Bingham, Alyssa S, George, Simon J, Cramer, Stephen P, Swartz, James R

“…The realization of hydrogenase-based technologies for renewable H(2) production is presently limited by the need for scalable and high-yielding methods to…”
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The HydG Enzyme Generates an Fe(CO)2(CN) Synthon in Assembly of the FeFe Hydrogenase H-Cluster by Kuchenreuther, Jon M., Myers, William K., Suess, Daniel L. M., Stich, Troy A., Pelmenschikov, Vladimir, Shiigi, Stacey A., Cramer, Stephen P., Swartz, James R., Britt, R. David, George, Simon J.

“…Three iron-sulfur proteins–HydE, HydF, and HydG–play a key role in the synthesis of the [2Fe]H component of the catalytic H-cluster of FeFe hydrogenase. The…”
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Cysteine as a ligand platform in the biosynthesis of the FeFe hydrogenase H cluster by Suess, Daniel L. M., Bürstel, Ingmar, De La Paz, Liliana, Kuchenreuther, Jon M., Pham, Cindy C., Cramer, Stephen P., Swartz, James R., Britt, R. David

“…Hydrogenases catalyze the redox interconversion of protons and H2, an important reaction for a number of metabolic processes and for solar fuel production. In…”
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New insights into [FeFe] hydrogenase activation and maturase function by Kuchenreuther, Jon M, Britt, R David, Swartz, James R

“…[FeFe] hydrogenases catalyze H(2) production using the H-cluster, an iron-sulfur cofactor that contains carbon monoxide (CO), cyanide (CN(-)), and a dithiolate…”
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Cell-free H-cluster synthesis and [FeFe] hydrogenase activation: all five CO and CN⁻ ligands derive from tyrosine by Kuchenreuther, Jon M, George, Simon J, Grady-Smith, Celestine S, Cramer, Stephen P, Swartz, James R

“…[FeFe] hydrogenases are promising catalysts for producing hydrogen as a sustainable fuel and chemical feedstock, and they also serve as paradigms for…”
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Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation by Kuchenreuther, Jon M, Stapleton, James A, Swartz, James R

“…[FeFe] hydrogenases are metalloenzymes involved in the anaerobic metabolism of H(2). These proteins are distinguished by an active site cofactor known as the…”
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Cell-free synthesis and maturation of [FeFe] hydrogenases by Boyer, Marcus E, Stapleton, James A, Kuchenreuther, Jon M, Wang, Chia-wei, Swartz, James R

“…[FeFe] hydrogenases catalyze the reversible reduction of protons to molecular hydrogen (Adams (1990); Biochim Biophys Acta 1020(2): 115-145) and are of…”
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The cyanide ligands of [FeFe] hydrogenase: pulse EPR studies of (13)C and (15)N-labeled H-cluster by Myers, William K, Stich, Troy A, Suess, Daniel L M, Kuchenreuther, Jon M, Swartz, James R, Britt, R David

“…The two cyanide ligands in the assembled cluster of [FeFe] hydrogenase originate from exogenous l-tyrosine. Using selectively labeled tyrosine substrates, the…”
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Biosynthesis of the [FeFe] Hydrogenase H Cluster: A Central Role for the Radical SAM Enzyme HydG by Suess, Daniel L. M, Kuchenreuther, Jon M, De La Paz, Liliana, Swartz, James R, Britt, R. David

“…Hydrogenase enzymes catalyze the rapid and reversible interconversion of H2 with protons and electrons. The active site of the [FeFe] hydrogenase is the H…”
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Cell-free synthesis of the H-cluster: a model for the in vitro assembly of metalloprotein metal centers by Kuchenreuther, Jon M, Shiigi, Stacey A, Swartz, James R

“…Many organometallic cofactors are highly complex and require multiple accessory proteins for both their assembly and transfer to a target protein. A cell-free…”
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Engineering Clostridium acetobutylicum for production of kerosene and diesel blendstock precursors by Bormann, Sebastian, Baer, Zachary C., Sreekumar, Sanil, Kuchenreuther, Jon M., Dean Toste, F., Blanch, Harvey W., Clark, Douglas S.

“…Processes for the biotechnological production of kerosene and diesel blendstocks are often economically unattractive due to low yields and product titers…”
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Assembly of erodible, DNA-containing thin films on the surfaces of polymer microparticles: Toward a layer-by-layer approach to the delivery of DNA to antigen-presenting cells by Saurer, Eric M., Jewell, Christopher M., Kuchenreuther, Jon M., Lynn, David M.

“…We report a layer-by-layer approach to the assembly of ultrathin and erodible DNA-containing films on the surfaces of polymer microparticles. DNA-containing…”
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The Cyanide Ligands of [FeFe] Hydrogenase: Pulse EPR Studies of 13C and 15N‑Labeled H‑Cluster by Myers, William K, Stich, Troy A, Suess, Daniel L. M, Kuchenreuther, Jon M, Swartz, James R, Britt, R. David

“…The two cyanide ligands in the assembled cluster of [FeFe] hydrogenase originate from exogenous l-tyrosine. Using selectively labeled tyrosine substrates, the…”
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The Cyanide Ligands of [FeFe] Hydrogenase: Pulse EPR Studies of 13 C and 15 N-Labeled H-Cluster by Myers, William K., Stich, Troy A., Suess, Daniel L. M., Kuchenreuther, Jon M., Swartz, James R., Britt, R. David

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Cell-free H-cluster Synthesis and [FeFe] Hydrogenase Activation: All Five CO and CN.sup.- Ligands Derive from Tyrosine by Kuchenreuther, Jon M, George, Simon J, Grady-Smith, Celestine S, Cramer, Stephen P, Swartz, James R

“…[FeFe] hydrogenases are promising catalysts for producing hydrogen as a sustainable fuel and chemical feedstock, and they also serve as paradigms for…”
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Nuclear Resonance Vibrational Spectroscopy and Electron Paramagnetic Resonance Spectroscopy of 57Fe-Enriched [FeFe] Hydrogenase Indicate Stepwise Assembly of the H‑Cluster by Kuchenreuther, Jon M, Guo, Yisong, Wang, Hongxin, Myers, William K, George, Simon J, Boyke, Christine A, Yoda, Yoshitaka, Alp, E. Ercan, Zhao, Jiyong, Britt, R. David, Swartz, James R, Cramer, Stephen P

“…The [FeFe] hydrogenase from Clostridium pasteurianum (CpI) harbors four Fe–S clusters that facilitate the transfer of an electron to the H-cluster, a…”
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Nuclear Resonance Vibrational Spectroscopy and Electron Paramagnetic Resonance Spectroscopy of 57 Fe-Enriched [FeFe] Hydrogenase Indicate Stepwise Assembly of the H-Cluster by Kuchenreuther, Jon M., Guo, Yisong, Wang, Hongxin, Myers, William K., George, Simon J., Boyke, Christine A., Yoda, Yoshitaka, Alp, E. Ercan, Zhao, Jiyong, Britt, R. David, Swartz, James R., Cramer, Stephen P.

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NRVS and EPR Spectroscopy of 57Fe-enriched [FeFe] Hydrogenase Indicate Stepwise Assembly of the H-cluster by Kuchenreuther, Jon M., Guo, Yisong, Wang, Hongxin, Myers, William K., George, Simon J., Boyke, Christine A., Yoda, Yoshitaka, Alp, E. Ercan, Zhao, Jiyong, Britt, R. David, Swartz, James R., Cramer, Stephen P.

“…The [FeFe] hydrogenase from Clostridium pasteurianum (CpI) harbors four Fe–S clusters that facilitate electron transfer to the H-cluster, a ligand-coordinated…”
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