Plasma concentration and local anesthetic activity of procaine hydrochloride following subcutaneous administration to horses

Plasma concentration and local anesthetic activity of procaine hydrochloride following subcutaneous administration to horses

To determine the durations of the local anesthetic effect and plasma procaine concentrations associated with 5- and 10-mg doses of procaine hydrochloride (with or without 100 microg of epinephrine) administered SC over the lateral palmar digital nerves of horses. 6 healthy adult horses. The hoof wit...

Full description

Saved in:
Bibliographic Details
Published in:American journal of veterinary research Vol. 68; no. 5; pp. 495 - 500
Main Authors: Kuchembuck, N.L, Colahan, P.T, Zientek, K.D, Pirman, D.A, Wegner, K, Cole, C.A
Format: Journal Article
Language:English
Published: United States 01-05-2007
Subjects:
analgesia
analgesic effect
Anesthesia, Local - veterinary
Anesthetics, Local - administration & dosage
Anesthetics, Local - blood
Anesthetics, Local - pharmacology
Animals
blood chemistry
Cross-Over Studies
dosage
dose response
Dose-Response Relationship, Drug
epinephrine
Female
horses
Horses - blood
Horses - physiology
Injections, Subcutaneous
local anesthetics
Male
pain
pharmacokinetics
procaine
Procaine - administration & dosage
Procaine - blood
Procaine - pharmacology
quantitative analysis
subcutaneous injection
Time Factors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine the durations of the local anesthetic effect and plasma procaine concentrations associated with 5- and 10-mg doses of procaine hydrochloride (with or without 100 microg of epinephrine) administered SC over the lateral palmar digital nerves of horses. 6 healthy adult horses. The hoof withdrawal reflex latency (HWRL) period was determined by use of a focused heat lamp before and after administration of procaine with and without epinephrine. Blood samples were collected immediately before determination of each HWRL period to assess plasma concentrations of procaine via liquid chromatography-mass spectrometry-mass spectrometry (LC-MS-MS). 10 but not 5 mg of procaine alone and 5 and 10 mg of procaine administered with epinephrine significantly prolonged the HWRL period (mean durations of effect, 5, 120 and 180 minutes, respectively), compared with baseline values. Plasma procaine concentrations did not correlate well with local anesthetic activity; for example, although the HWRL was prolonged to the maximum permitted duration of 20 seconds at 60 to 180 minutes following administration of the 5-mg dose of procaine with epinephrine in certain horses, plasma procaine concentrations were less than the limit of quantitation of the LC-MS-MS assay. Small doses of procaine coadministered with epinephrine provided long-lasting local analgesia and resulted in plasma procaine concentrations that were not always detectable via LC-MS-MS. On the basis of these results, the use of regulatory limits or thresholds for procaine concentration in equine plasma samples obtained after racing should be seriously reconsidered.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-News-1
ObjectType-Feature-3
content type line 23
ISSN:0002-9645
1943-5681
DOI:10.2460/ajvr.68.5.495