Insulin receptor tyrosine kinase activity and substrate 1 (IRS-1) expression in human myometrium and leiomyoma

Uterine leiomyomas are the commonest tumors of the genital tract. Growth factors seem to be implicated in the development of leiomyoma. To determine the insulin receptor (IR) tyrosine kinase activity – phosphorylation of exogenous substrate poly(Glu 4: Tyr 1) – and insulin receptor substrate 1 expre...

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Published in:	European journal of obstetrics & gynecology and reproductive biology Vol. 123; no. 1; pp. 107 - 110
Main Authors:	Orcy, Rafael B., Brum, Ilma, Silva, Roselis S.M. da, Kucharski, Luiz C.R., E. Corleta, Helena v., Capp, Edison
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 01-11-2005 Elsevier
Subjects:	Animals Biological and medical sciences Female Gynecology. Andrology. Obstetrics Humans In Vitro Techniques Insulin - pharmacology Insulin receptor Insulin Receptor Substrate Proteins IRS-1 Leiomyoma Leiomyoma - metabolism Medical sciences Muscle, Skeletal - metabolism Myometrium Myometrium - drug effects Myometrium - metabolism Phosphoproteins - metabolism Phosphorylation Protein-Tyrosine Kinases - metabolism Rats Rats, Wistar Reference Values Tyrosine kinase Uterine Neoplasms - metabolism Leiomyoma Tyrosine kinase Myometrium Insulin receptor IRS-1 Human Enzyme Transferases Gynecology Receptor tyrosine kinase Smooth muscle Obstetrics Biological activity Substrate Insulin receptor substrate-1 gene Uterus Female genital system Benign neoplasm Protein-tyrosine kinase Hormonal receptor
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Summary:	Uterine leiomyomas are the commonest tumors of the genital tract. Growth factors seem to be implicated in the development of leiomyoma. To determine the insulin receptor (IR) tyrosine kinase activity – phosphorylation of exogenous substrate poly(Glu 4: Tyr 1) – and insulin receptor substrate 1 expression in normal myometrium and leiomyoma. The study group consisted of 12 women with leiomyoma undergoing routine hysterectomy. Samples of leiomyoma and adjacent normal myometrium were obtained at the time of operation. Plasma membrane fractions were prepared and samples were incubated with and without insulin and incubated with exogenous substrate poly(Glu 4: Tyr 1). IRS-1 expression was studied in the whole lysate via Western blotting using specific antibodies. Data were analyzed using Student's t-test. The phosphorylation of the exogenous substrate poly(Glu 4: Tyr 1) in myometrium (1.566 ± 0.177) and in leiomyoma (1.98 ± 0.612) were similar ( P = 0.774). The IRS-1 levels in myometrium (0.190 ± 0.022) and in leiomyoma (0.226 ± 0.022) were not different ( P = 0.184). There was no difference in IR tyrosine kinase activity (phosphorylation of exogenous substrate) and IRS-1 expression between normal myometrium and leiomyomata. Other steps in the insulin signaling cascade require further study to investigate the role of insulin receptor in leiomyomata.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0301-2115 1872-7654
DOI:	10.1016/j.ejogrb.2005.02.025