Hiostopathological and Immnohistochemical studies on an effect of vaccine for Ayu vibriosis
In this study the effect of immersion vaccination of Ayu vibriosis was investigated from the histopathological and immunohistochemical points of view, and the infection mechanism of vibriosis as well as the cause of death were proposed. Ayu, Plecoglossus altivelis, were vaccinated by the immersion i...
Saved in:
| Published in: | Gyobyō kenkyu Vol. 23; no. 2; pp. 85 - 90 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | Japanese |
| Published: |
01-01-1988
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | In this study the effect of immersion vaccination of Ayu vibriosis was investigated from the histopathological and immunohistochemical points of view, and the infection mechanism of vibriosis as well as the cause of death were proposed. Ayu, Plecoglossus altivelis, were vaccinated by the immersion in suspension of formalin-killed Vibrio anguillarum at concentration of 8.6 .times. 106 CFU /ml for 1 hour. The experimental fish were estimated 60 days after the vaccination. The water temperature was 20 to 22.degree. C during the course of experiment. The cumulative mortality rate of the vaccinated group was lower by 40% than the control group when being challenged with immersion of 10 minutes in suspension of V. anguillarum at concentration of 3.4 times. 10 super(4) CFU/ml, and by 20% at concentration of 3.4 times. 105 CFU/ml. In control group, lesions and bacterial proliferation were detected first in the skin, then in other organs through the whole body. Thus, the mechanism of infection was proposed as following. The bacterium first penetrated through the epidermis to the dermis where it proliferated and caused lesions. The bacterial invasion into the blood stream through local capillary systems finally induced the systemic infection. The cause of death of the experimental fish was considered to be the heart and breath failure resulting from bacteremia. In the vaccinated group, when compared with the control, skin lesions were fewer in number and less severe in all individuals. When examined histologically, the skin lesions appeared later in the vaccinated group than the control, and a more extensive cellular infiltration with a reduced number of bacteria was observed in the former group. The results suggested that the vaccination strengthened the resistant properties of the epidermis and accelerated the cellular infiltration in the dermis of the host so that the bacterial penetration and proliferation were inhibited. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2 |
| ISSN: | 0388-788X 1881-7335 |