Crosslinking of (Cytosine-5)-DNA Methyltransferase SsoII and its Complexes with Specific DNA Duplexes Provides an Insight into Their Structures

(Cytosine-5)-DNA methyltransferase SsoII (M.SsoII) functions as a methyltransferase and also as a transcription factor. Chemical and photochemical crosslinking was used for exploring the structure of M.SsoII-DNA complexes and M.SsoII in the absence of DNA. Photocrosslinking with 4-(N-maleimido)benzo...

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Bibliographic Details
Published in:	Nucleosides, nucleotides & nucleic acids Vol. 30; no. 7-8; pp. 632 - 650
Main Authors:	Ryazanova, Alexandra Yurievna, Winkler, Ines, Friedhoff, Peter, Viryasov, Mikhail Borisovich, Oretskaya, Tatiana Semenovna, Kubareva, Elena Aleksandrovna
Format:	Journal Article
Language:	English
Published:	United States Taylor & Francis Group 01-07-2011
Subjects:	Base Sequence Cross-Linking Reagents - chemistry DNA - chemistry DNA methyltransferase SsoII DNA-Cytosine Methylases - chemistry Models, Molecular Molecular Sequence Data Photochemical Processes Protein Conformation protein-DNA complexes protein-DNA crosslinking protein-protein crosslinking Recombinant Proteins - chemistry Shigella sonnei - enzymology
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Summary:	(Cytosine-5)-DNA methyltransferase SsoII (M.SsoII) functions as a methyltransferase and also as a transcription factor. Chemical and photochemical crosslinking was used for exploring the structure of M.SsoII-DNA complexes and M.SsoII in the absence of DNA. Photocrosslinking with 4-(N-maleimido)benzophenone demonstrated that in the M.SsoII complex with DNA containing the regulatory site, the M.SsoII region responsible for methylation was bound to DNA flanking the regulatory site, which contained no methylation sequence. This required high flexibility of the linker connecting the M.SsoII N-terminal domain and the M.SsoII region responsible for methylation. The flexibility was demonstrated by crosslinking with bis-maleimidoethane and 1,11-bis-maleimidotetraethyleneglycol.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1525-7770 1532-2335
DOI:	10.1080/15257770.2011.584339