Search Results - "Kühn, Ralf"

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells by Chu, Van Trung, Weber, Timm, Wefers, Benedikt, Wurst, Wolfgang, Sander, Sandrine, Rajewsky, Klaus, Kühn, Ralf

“…The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway. The insertion of precise genetic…”
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Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function by Piwecka, Monika, Glažar, Petar, Hernandez-Miranda, Luis R., Memczak, Sebastian, Wolf, Susanne A., Rybak-Wolf, Agnieszka, Filipchyk, Andrei, Klironomos, Filippos, Jara, Cledi Alicia Cerda, Fenske, Pascal, Trimbuch, Thorsten, Zywitza, Vera, Plass, Mireya, Schreyer, Luisa, Ayoub, Salah, Kocks, Christine, Kühn, Ralf, Rosenmund, Christian, Birchmeier, Carmen, Rajewsky, Nikolaus

“…Hundreds of circular RNAs (circRNAs) are highly abundant in the mammalian brain, often with conserved expression. Here we show that the circRNA Cdr1as is…”
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Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity by Fecher, Caroline, Trovò, Laura, Müller, Stephan A., Snaidero, Nicolas, Wettmarshausen, Jennifer, Heink, Sylvia, Ortiz, Oskar, Wagner, Ingrid, Kühn, Ralf, Hartmann, Jana, Karl, Rosa Maria, Konnerth, Arthur, Korn, Thomas, Wurst, Wolfgang, Merkler, Doron, Lichtenthaler, Stefan F., Perocchi, Fabiana, Misgeld, Thomas

“…Mitochondria vary in morphology and function in different tissues; however, little is known about their molecular diversity among cell types. Here we…”
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Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes by Chu, Van Trung, Weber, Timm, Graf, Robin, Sommermann, Thomas, Petsch, Kerstin, Sack, Ulrike, Volchkov, Pavel, Rajewsky, Klaus, Kühn, Ralf

“…The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less…”
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Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases by Meyer, Melanie, de Angelis, Martin Hrabé, Wurst, Wolfgang, Kühn, Ralf, Chambon, Pierre

“…Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack…”
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Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line by Chu, Van Trung, Graf, Robin, Wirtz, Tristan, Weber, Timm, Favret, Jeremy, Li, Xun, Petsch, Kerstin, Tran, Ngoc Tung, Sieweke, Michael H., Berek, Claudia, Kühn, Ralf, Rajewsky, Klaus

“…Applying clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9)-mediated mutagenesis to primary mouse immune…”
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Mutations in Disordered Regions Can Cause Disease by Creating Dileucine Motifs by Meyer, Katrina, Kirchner, Marieluise, Uyar, Bora, Cheng, Jing-Yuan, Russo, Giulia, Hernandez-Miranda, Luis R., Szymborska, Anna, Zauber, Henrik, Rudolph, Ina-Maria, Willnow, Thomas E., Akalin, Altuna, Haucke, Volker, Gerhardt, Holger, Birchmeier, Carmen, Kühn, Ralf, Krauss, Michael, Diecke, Sebastian, Pascual, Juan M., Selbach, Matthias

“…Many disease-causing missense mutations affect intrinsically disordered regions (IDRs) of proteins, but the molecular mechanism of their pathogenicity is…”
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Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome by Inak, Gizem, Rybak-Wolf, Agnieszka, Lisowski, Pawel, Pentimalli, Tancredi M., Jüttner, René, Glažar, Petar, Uppal, Karan, Bottani, Emanuela, Brunetti, Dario, Secker, Christopher, Zink, Annika, Meierhofer, David, Henke, Marie-Thérèse, Dey, Monishita, Ciptasari, Ummi, Mlody, Barbara, Hahn, Tobias, Berruezo-Llacuna, Maria, Karaiskos, Nikos, Di Virgilio, Michela, Mayr, Johannes A., Wortmann, Saskia B., Priller, Josef, Gotthardt, Michael, Jones, Dean P., Mayatepek, Ertan, Stenzel, Werner, Diecke, Sebastian, Kühn, Ralf, Wanker, Erich E., Rajewsky, Nikolaus, Schuelke, Markus, Prigione, Alessandro

“…Leigh syndrome (LS) is a severe manifestation of mitochondrial disease in children and is currently incurable. The lack of effective models hampers our…”
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Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth by Dzamukova, Maria, Brunner, Tobias M., Miotla-Zarebska, Jadwiga, Heinrich, Frederik, Brylka, Laura, Mashreghi, Mir-Farzin, Kusumbe, Anjali, Kühn, Ralf, Schinke, Thorsten, Vincent, Tonia L., Löhning, Max

“…Bone growth requires a specialised, highly angiogenic blood vessel subtype, so-called type H vessels, which pave the way for osteoblasts surrounding these…”
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Oscillations of MyoD and Hes1 proteins regulate the maintenance of activated muscle stem cells by Lahmann, Ines, Bröhl, Dominique, Zyrianova, Tatiana, Isomura, Akihiro, Czajkowski, Maciej T, Kapoor, Varun, Griger, Joscha, Ruffault, Pierre-Louis, Mademtzoglou, Despoina, Zammit, Peter S, Wunderlich, Thomas, Spuler, Simone, Kühn, Ralf, Preibisch, Stephan, Wolf, Jana, Kageyama, Ryoichiro, Birchmeier, Carmen

“…The balance between proliferation and differentiation of muscle stem cells is tightly controlled, ensuring the maintenance of a cellular pool needed for muscle…”
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A homology independent sequence replacement strategy in human cells using a CRISPR nuclease by Danner, Eric, Lebedin, Mikhail, de la Rosa, Kathrin, Kühn, Ralf

“…Precision genomic alterations largely rely on homology directed repair (HDR), but targeting without homology using the non-homologous end-joining (NHEJ)…”
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The Parkinson’s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 by Funk, Natalja, Munz, Marita, Ott, Thomas, Brockmann, Kathrin, Wenninger-Weinzierl, Andrea, Kühn, Ralf, Vogt-Weisenhorn, Daniela, Giesert, Florian, Wurst, Wolfgang, Gasser, Thomas, Biskup, Saskia

“…Mutations within Leucine-rich repeat kinase 2 (LRRK2) are associated with late-onset Parkinson’s disease. The physiological function of LRRK2 and molecular…”
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CRISPR-Cas9-Mediated ELANE Mutation Correction in Hematopoietic Stem and Progenitor Cells to Treat Severe Congenital Neutropenia by Tran, Ngoc Tung, Graf, Robin, Wulf-Goldenberg, Annika, Stecklum, Maria, Strauß, Gabriele, Kühn, Ralf, Kocks, Christine, Rajewsky, Klaus, Chu, Van Trung

“…Severe congenital neutropenia (SCN) is a monogenic disorder. SCN patients are prone to recurrent life-threatening infections. The main causes of SCN are…”
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Novel human neurodevelopmental and neurodegenerative disease associated with IRF2BPL gene variants-mechanisms and therapeutic avenues by Bauersachs, Daniel, Bomholtz, Louise, Del Rey Mateos, Sara, Kühn, Ralf, Lisowski, Pawel

“…Recently a broad range of phenotypic abnormalities related to the neurodevelopmental and neurodegenerative disorder NEDAMSS (Neurodevelopmental Disorder with…”
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Prox2 and Runx3 vagal sensory neurons regulate esophageal motility by Lowenstein, Elijah D., Ruffault, Pierre-Louis, Misios, Aristotelis, Osman, Kate L., Li, Huimin, Greenberg, Rachel S., Thompson, Rebecca, Song, Kun, Dietrich, Stephan, Li, Xun, Vladimirov, Nikita, Woehler, Andrew, Brunet, Jean-François, Zampieri, Niccolò, Kühn, Ralf, Liberles, Stephen D., Jia, Shiqi, Lewin, Gary R., Rajewsky, Nikolaus, Lever, Teresa E., Birchmeier, Carmen

“…Vagal sensory neurons monitor mechanical and chemical stimuli in the gastrointestinal tract. Major efforts are underway to assign physiological functions to…”
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Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA by Wefers, Benedikt, Panda, Sudeepta K, Ortiz, Oskar, Brandl, Christina, Hensler, Svenja, Hansen, Jens, Wurst, Wolfgang, Kühn, Ralf

“…Genetically engineered mice are instrumental for the analysis of mammalian gene function in health and disease. As classical gene targeting, which is performed…”
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Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors by Tran, Ngoc-Tung, Bashir, Sanum, Li, Xun, Rossius, Jana, Chu, Van Trung, Rajewsky, Klaus, Kühn, Ralf

“…The CRISPR-Cas9 system is used for genome editing in mammalian cells by introducing double-strand breaks (DSBs) which are predominantly repaired via…”
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Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides by Wefers, Benedikt, Meyer, Melanie, Ortiz, Oskar, de Angelis, Martin Hrabé, Hansen, Jens, Wurst, Wolfgang, Kühn, Ralf

“…The study of genetic disease mechanisms relies mostly on targeted mouse mutants that are derived from engineered embryonic stem (ES) cells. Nevertheless, the…”
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Inducible gene deletion in astroglia and radial glia-A valuable tool for functional and lineage analysis by Mori, Tetsuji, Tanaka, Kohichi, Buffo, Annalisa, Wurst, Wolfgang, Kühn, Ralf, Götz, Magdalena

“…Astrocytes are thought to play a variety of key roles in the adult brain, such as their participation in synaptic transmission, in wound healing upon brain…”
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Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination by Bashir, Sanum, Dang, Tu, Rossius, Jana, Wolf, Johanna, Kühn, Ralf

“…Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand…”
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