SSAP markers based on a novel Ty1-copia like element are a powerful tool for the assessment of genetic diversity in rye (Secale cereale L.) inbred lines

SSAP markers based on a novel Ty1-copia like element are a powerful tool for the assessment of genetic diversity in rye (Secale cereale L.) inbred lines

A partial sequence of a Ty1-copia retrotransposon was isolated from the genome of the rye (Secale cereale L.) inbred line L318 using degenerate primers targeted at the RNaseH sequence. Its putative 3’LTR region exhibited similarity to LTRs of known plant retrotransposons such as BARE-1, while no s...

Full description

Saved in:
Bibliographic Details
Published in:Cereal research communications Vol. 40; no. 2; pp. 204 - 209
Main Authors: Bolibok-Bragoszewska, H., Zabierzewska, N., Hromada-Judycka, A., Krzewska, L.
Format: Journal Article
Language:English
Published: Cham Akadémiai Kiadà 01-06-2012
AKADÉMIAI KIADÓ
Springer International Publishing
Subjects:
Agriculture
cluster analysis
Coefficients
DNA
Genetic diversity
genetic variation
Genetics
genome
Genomes
Genomics
inbred lines
Inbred strains
Life Sciences
Molecular genetics
molecular markers
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Physiology
Power tools
retrotransposon
Retrotransposons
Rye
Secale cereale
SSAP
Ty1-copia
genetic diversity
SSAP
retrotransposon
molecular markers
rye
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A partial sequence of a Ty1-copia retrotransposon was isolated from the genome of the rye (Secale cereale L.) inbred line L318 using degenerate primers targeted at the RNaseH sequence. Its putative 3’LTR region exhibited similarity to LTRs of known plant retrotransposons such as BARE-1, while no significant similarity to known rye retrotransposon sequences was found. A Sequence Specific Amplification Polymorphism (SSAP) protocol employing primer designed to correspond to the putative polypurine tract was developed and used for analysis of 30 rye inbred lines. Values of the parameters calculated to evaluate the efficiency of the method as well as the results of cluster analysis demonstrate that it is a powerful tool for genetic diversity analyses of rye.
Bibliography:http://dx.doi.org/10.1556%2FCRC.40.2012.2.4
ISSN:0133-3720
1788-9170
DOI:10.1556/CRC.40.2012.2.4