Comparative transmission of bacteria from Artemia salina and Brachionus plicatilis to the cnidarian Nematostella vectensis

ABSTRACT The microbial community associated with animals (microbiome) is essential for development, physiology, and health of host organisms. A critical step to understand the assembly of microbiomes is to determine how effectively bacteria colonize and establish within the host. Bacteria commonly c...

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Bibliographic Details
Published in:	FEMS microbiology ecology Vol. 98; no. 10
Main Authors:	Krueger, Quinton A, Shore, Madisun H, Reitzel, Adam M
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 29-09-2022
Subjects:	Animals Artemia Artemia - microbiology Artemia salina Bacteria Bacteria - genetics Brachionus plicatilis Disease transmission Ecology Food consumption Inoculation Larva - microbiology Larvae Microbiology Microbiomes Microorganisms Nematostella vectensis Prey Rotifera Sea Anemones Transplantation cnidarian prey bacteria marine invertebrates colonization predator
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Summary:	ABSTRACT The microbial community associated with animals (microbiome) is essential for development, physiology, and health of host organisms. A critical step to understand the assembly of microbiomes is to determine how effectively bacteria colonize and establish within the host. Bacteria commonly colonize hosts through vertical transmission, passively from the environment, or through food consumption. Using the prey feeding method (PFM), we test transmittance of Bacillus velezensis, Pseudoalteromonas spiralis, and Vibrio alginolyticus to Nematostella vectensis using two prey, Artemia salina and Brachionus plicatilis. We compare PFM to a solution uptake method (SUM) to quantify the concentration of bacteria in these host organisms, with plate counts. Larvae had a similar uptake with SUM at 6 h but had greater concentrations at 48 h versus PFM. Juveniles acquired similar concentrations at 6 h for SUM and PFM using B. plicatilis and A. salina. At 2 days, the quantity of bacteria vectored from PFM increased. After 7 days the CFUs decreased 2-fold with B. plicatilis and A. salina relative to the 2-day concentrations, and further decreased after 14 days. Therefore, prey-mediated methods provide greater microbe transplantation than SUM after 24 h, supporting this approach as a more successful inoculation method of individual bacterial species. This study quantified bacterial transmittance from prey to predator in a simple estuarine food web.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1574-6941 0168-6496 1574-6941
DOI:	10.1093/femsec/fiac096