Simultaneous Analysis of D- and L-Serine in Cerebrospinal Fluid by Use of HPLC

D-Serine is a coagonist for the glycine-binding site of the N-methyl-D-aspartate receptors and has been implicated in various neuropsychiatric functions such as learning, memory, and nociception, as well as schizophrenia and Alzheimer disease. We developed an HPLC method for D- and L-serine in cereb...

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Bibliographic Details
Published in:	Clinical chemistry (Baltimore, Md.) Vol. 53; no. 8; pp. 1489 - 1494
Main Authors:	Sethuraman, Rama, Krishnamoorthy, Malathi G, Lee, Tat-Leang, Liu, Eugene Hern C, Chiang, Siau, Nishimura, Wataru, Sakai, Masato, Minami, Toshiaki, Tachibana, Shinro
Format:	Journal Article
Language:	English
Published:	Washington, DC Am Assoc Clin Chem 01-08-2007 American Association for Clinical Chemistry Oxford University Press
Subjects:	Age differences Alzheimer's disease Amino acids Analytical, structural and metabolic biochemistry Binding sites Biological and medical sciences Body fluids Chromatography, High Pressure Liquid Clinical medicine Correlation analysis Fundamental and applied biological sciences. Psychology Humans Indicators and Reagents Investigative techniques, diagnostic techniques (general aspects) Liquid chromatography Medical research Medical sciences Mental disorders Methods Nervous system Nitric oxide Osteoarthritis p-Dimethylaminoazobenzene - analogs & derivatives Patients Reproducibility of Results Serine - cerebrospinal fluid Serine - chemistry Spectrophotometry Spinal cord Stereoisomerism Aminoacid Serine Clinical biology HPLC chromatography Biochemistry Cerebrospinal fluid Molecular biology
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Summary:	D-Serine is a coagonist for the glycine-binding site of the N-methyl-D-aspartate receptors and has been implicated in various neuropsychiatric functions such as learning, memory, and nociception, as well as schizophrenia and Alzheimer disease. We developed an HPLC method for D- and L-serine in cerebrospinal fluid (CSF). The dabsylated racemic serine peak, automatically collected using a previously reported HPLC separation process for CSF amino acids, was desalted and subjected to a chiral resolution HPLC step with a Sumichiral column using an ultraviolet-visible detector. The limits of quantification (signal-to-noise ratio = 10) for D- and L-serine were 0.8 and 1.3 micromol/L, respectively. The mean imprecision values (CVs) for within-day measurements of D- and L-serine were 2.1% and 1.8%, respectively, and for between-day were 6.2% and 6.6%. Mean recovery of CSF serine (sum of D-serine + L-serine) applied to the Sumichiral column was 87%. The mean (SD) d-serine concentrations in 45 CSF samples obtained from 16 patients with chronic pain due to degenerative osteoarthritis of the knees, 16 with postherpetic neuralgia, and 13 with no pain were, respectively, 3.97 (0.44), 1.85 (0.21), and 2.72 (0.32) micromol/L. D- and L-serine can be quantified with ultraviolet-visible detection of dabsyl derivatives. The dabsyl derivatives are stable and allow duplicate analysis of CSF samples in multisample runs.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0009-9147 1530-8561
DOI:	10.1373/clinchem.2007.086702