Comparative transcriptome analysis provides novel insights into molecular response of salt-tolerant and sensitive polyembryonic mango genotypes to salinity stress at seedling stage

Comparative transcriptome analysis provides novel insights into molecular response of salt-tolerant and sensitive polyembryonic mango genotypes to salinity stress at seedling stage

Increased soil salinity in the recent years has adversely affected the productivity of mango globally. Extending the cultivation of mango in salt affected regions warrants the use of salinity tolerant/resistant rootstocks. However, the lack of sufficient genomic and transcriptomic information impede...

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Published in:Frontiers in plant science Vol. 14; p. 1152485
Main Authors: Perveen, Nusrat, Dinesh, M R, Sankaran, M, Ravishankar, K V, Krishnajee, Hara Gopal, Hanur, Vageeshbabu S, Alamri, Saud, Kesawat, Mahipal Singh, Irfan, Mohammad
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 12-04-2023
Subjects:
mango
Mylepelian
Plant Science
polyembryony
RNA-Seq
salinity stress
transcriptome
Mylepelian
RNA-Seq
salinity stress
transcriptome
SOS pathway
Turpentine
polyembryony
mango
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Summary:Increased soil salinity in the recent years has adversely affected the productivity of mango globally. Extending the cultivation of mango in salt affected regions warrants the use of salinity tolerant/resistant rootstocks. However, the lack of sufficient genomic and transcriptomic information impedes comprehensive research at the molecular level. We employed RNA sequencing-based transcriptome analysis to gain insight into molecular response to salt stress by using two polyembryonic mango genotypes with contrasting response to salt stress viz., salt tolerant Turpentine and salt susceptible Mylepelian. RNA sequencing by Novaseq6000 resulted in a total of 2795088, 17535948, 7813704 and 5544894 clean reads in Mylepelian treated (MT), Mylepelian control (MC), Turpentine treated (TT) and Turpentine control (TC) respectively. In total, 7169 unigenes annotated against all the five public databases, including NR, NT, PFAM, KOG, Swissport, KEGG and GO. Further, maximum number of differentially expressed genes were found between MT and MC (2106) followed by MT vs TT (1158) and TT and TC (587). The differentially expressed genes under different treatment levels included transcription factors (bZIP, NAC, bHLH), genes involved in Calcium-dependent protein kinases (CDPKs), ABA biosynthesis, Photosynthesis etc. Expression of few of these genes was experimentally validated through quantitative real-time PCR (qRT-PCR) and contrasting expression pattern of Auxin Response Factor 2 (ARF2), Late Embryogenesis Abundant (LEA) and CDPK genes were observed between Turpentine and Mylepelian. The results of this study will be useful in understanding the molecular mechanism underlying salt tolerance in mango which can serve as valuable baseline information to generate new targets in mango breeding for salt tolerance.
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Reviewed by: Deepti Nigam, University of Nebraska-Lincoln, United States; Mohammed Ali Abd Elhammed Abd Allah, Desert Research Center, Egypt
Edited by: Dev Mani Pandey, Birla Institute of Technology, India
This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1152485