Mediatorless Carbohydrate/Oxygen Biofuel Cells with Improved Cellobiose Dehydrogenase Based Bioanode

Direct electron transfer (DET) between cellobiose dehydrogenase from Humicola insolens ascomycete (HiCDH) and gold nanoparticles (AuNPs) was achieved by modifying AuNPs with a novel, positively charged thiol N‐(6‐mercapto)hexylpyridinium (MHP). The DET enabled the use of the HiCDH enzyme as an anodi...

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Bibliographic Details
Published in:	Fuel cells (Weinheim an der Bergstrasse, Germany) Vol. 14; no. 6; pp. 792 - 800
Main Authors:	Krikstolaityte, V., Lamberg, P., Toscano, M. D., Silow, M., Eicher-Lorka, O., Ramanavicius, A., Niaura, G., Abariute, L., Ruzgas, T., Shleev, S.
Format:	Journal Article
Language:	English
Published:	Weinheim WILEY-VCH Verlag 01-12-2014 WILEY‐VCH Verlag Wiley Subscription Services, Inc
Subjects:	Bilirubin Oxidase Biodiesel fuels Catalysts Cellobiose Dehydrogenase Direct Electron Transfer Enzymatic Fuel Cell Enzymes Fuel cells Gold Nanoparticle N-(6-mercapto)hexylpyridinium Nanoparticles
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Summary:	Direct electron transfer (DET) between cellobiose dehydrogenase from Humicola insolens ascomycete (HiCDH) and gold nanoparticles (AuNPs) was achieved by modifying AuNPs with a novel, positively charged thiol N‐(6‐mercapto)hexylpyridinium (MHP). The DET enabled the use of the HiCDH enzyme as an anodic biocatalyst in the design of a mediatorless carbohydrate/oxygen enzymatic fuel cell (EFC). A biocathode of the EFC was based on bilirubin oxidase from Myrothecium verrucaria (MvBOx) directly immobilised on the surface of AuNPs. The following parameters of the EFC based on Au/AuNP/MHP/HiCDH bioanode and Au/AuNP/MvBOx biocathode were obtained in quiescent air saturated PBS, pH 7.4, containing: (i) 5 mM glucose‐open‐circuit voltage (OCV) of 0.65 ± 0.011 V and the maximal power density of 4.77 ± 1.34 μW cm−2 at operating voltage of 0.50 V; or (ii) 10 mM lactose‐OCV of 0.67 ± 0.006 V and the maximal power density of 8.64 ± 1.91 μW cm−2 at operating voltage of 0.50 V. The half‐life operation times of the EFC were estimated to be at least 13 and 44 h in air saturated PBS containing 5 mM glucose and 10 mM lactose, respectively. Among advantages of HiCDH/MvBOx FCs are (i) simplified construction, (ii) relatively high power output with glucose as biofuel, and (iii) the absence of the inhibition of the HiCDH based bioanode by lactose, when compared with the best previously reported CDH based bioanode.
Bibliography:	ArticleID:FUCE201400003 istex:86E3324FA297B2A68857A1C1A06E9F58CD012051 Swedish Institute ark:/67375/WNG-932SC23M-W Russian Foundation for Basic Research Swedish Research Council European Commission
ISSN:	1615-6846 1615-6854 1615-6854
DOI:	10.1002/fuce.201400003