Search Results - "Krez, Nadja"

Construction and validation of safe Clostridium botulinum Group II surrogate strain producing inactive botulinum neurotoxin type E toxoid by Nowakowska, Maria B., Selby, Katja, Przykopanski, Adina, Krüger, Maren, Krez, Nadja, Dorner, Brigitte G., Dorner, Martin B., Jin, Rongsheng, Minton, Nigel P., Rummel, Andreas, Lindström, Miia

“…Botulinum neurotoxins (BoNTs), produced by the spore-forming bacterium Clostridium botulinum , cause botulism, a rare but fatal illness affecting humans and…”
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Structural basis for botulinum neurotoxin E recognition of synaptic vesicle protein 2 by Liu, Zheng, Lee, Pyung-Gang, Krez, Nadja, Lam, Kwok-ho, Liu, Hao, Przykopanski, Adina, Chen, Peng, Yao, Guorui, Zhang, Sicai, Tremblay, Jacqueline M., Perry, Kay, Shoemaker, Charles B., Rummel, Andreas, Dong, Min, Jin, Rongsheng

“…Botulinum neurotoxin E (BoNT/E) is one of the major causes of human botulism and paradoxically also a promising therapeutic agent. Here we determined the…”
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A viral-fusion-peptide-like molecular switch drives membrane insertion of botulinum neurotoxin A1 by Lam, Kwok-ho, Guo, Zhuojun, Krez, Nadja, Matsui, Tsutomu, Perry, Kay, Weisemann, Jasmin, Rummel, Andreas, Bowen, Mark E., Jin, Rongsheng

“…Botulinum neurotoxin (BoNT) delivers its protease domain across the vesicle membrane to enter the neuronal cytosol upon vesicle acidification. This process is…”
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Botulinum neurotoxin C mutants reveal different effects of syntaxin or SNAP-25 proteolysis on neuromuscular transmission by Zanetti, Giulia, Sikorra, Stefan, Rummel, Andreas, Krez, Nadja, Duregotti, Elisa, Negro, Samuele, Henke, Tina, Rossetto, Ornella, Binz, Thomas, Pirazzini, Marco

“…Botulinum neurotoxin serotype C (BoNT/C) is a neuroparalytic toxin associated with outbreaks of animal botulism, particularly in birds, and is the only BoNT…”
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A camelid single-domain antibody neutralizes botulinum neurotoxin A by blocking host receptor binding by Yao, Guorui, Lam, Kwok-ho, Weisemann, Jasmin, Peng, Lisheng, Krez, Nadja, Perry, Kay, Shoemaker, Charles B., Dong, Min, Rummel, Andreas, Jin, Rongsheng

“…Antibody treatment is currently the only available countermeasure for botulism, a fatal illness caused by flaccid paralysis of muscles due to botulinum…”
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Generation and Characterization of Six Recombinant Botulinum Neurotoxins as Reference Material to Serve in an International Proficiency Test by Weisemann, Jasmin, Krez, Nadja, Fiebig, Uwe, Worbs, Sylvia, Skiba, Martin, Endermann, Tanja, Dorner, Martin B, Bergström, Tomas, Muñoz, Amalia, Zegers, Ingrid, Müller, Christian, Jenkinson, Stephen P, Avondet, Marc-Andre, Delbrassinne, Laurence, Denayer, Sarah, Zeleny, Reinhard, Schimmel, Heinz, Åstot, Crister, Dorner, Brigitte G, Rummel, Andreas

“…The detection and identification of botulinum neurotoxins (BoNT) is complex due to the existence of seven serotypes, derived mosaic toxins and more than 40…”
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Recombinant Expression of BoNT Molecules in E coli and Determination of Biological Activity by ex vivo Mouse Phrenic Nerve Hemidiaphragm Assay by Weisemann, Jasmin, Krez, Nadja, Bundtzen, Laura, Rummel, Andreas

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Anthelmintic and anticonvulsive drugs do not alter the biological activity of BoNT in neurons by Mahrhold, Stefan, Krez, Nadja, Rummel, Andreas

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Neutralisation of specific surface carboxylates speeds up translocation of botulinum neurotoxin type B enzymatic domain by Pirazzini, Marco, Henke, Tina, Rossetto, Ornella, Mahrhold, Stefan, Krez, Nadja, Rummel, Andreas, Montecucco, Cesare, Binz, Thomas

“…•Three specific surface carboxylates of BoNT/B were neutralised by mutation to amide.•The triple mutant expedites entry of the enzymatic domain into…”
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Identification of the synaptic vesicle glycoprotein 2 receptor binding site in botulinum neurotoxin A by Strotmeier, Jasmin, Mahrhold, Stefan, Krez, Nadja, Janzen, Constantin, Lou, Jianlong, Marks, James D., Binz, Thomas, Rummel, Andreas

“…•Neutralising mechanism of potent therapeutic monoclonal BoNT/A antibody is exhibited.•Single mutation G1292R eliminates interaction of BoNT/A with all three…”
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Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG‐Syt1‐SV2 plasma membrane nanocluster for synaptic vesicle entry by Joensuu, Merja, Syed, Parnayan, Saber, Saber H, Lanoue, Vanessa, Wallis, Tristan P, Rae, James, Blum, Ailisa, Gormal, Rachel S, Small, Christopher, Sanders, Shanley, Jiang, Anmin, Mahrhold, Stefan, Krez, Nadja, Cousin, Michael A, Cooper‐White, Ruby, Cooper‐White, Justin J, Collins, Brett M, Parton, Robert G, Balistreri, Giuseppe, Rummel, Andreas, Meunier, Frédéric A

“…The unique nerve terminal targeting of botulinum neurotoxin type A (BoNT/A) is due to its capacity to bind two receptors on the neuronal plasma membrane:…”
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A functional assay devoid of antibodies for serotyping and detection of botulinum neurotoxins by Weisemann, Jasmin, Krez, Nadja, Stevens, Gregory, Rummel, Andreas

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BoNT/AB hybrid maintains similar duration of paresis as BoNT/A wild-type in murine running wheel assay by Kutschenko, Anna, Reinert, Marie-Christine, Krez, Nadja, Liebetanz, David, Rummel, Andreas

“…•Of all BoNT serotypes BoNT/A has the highest potency but low binding affinity.•BoNT/B displays a higher affinity to neuronal membranes.•To combine the best…”
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Fast automatic detection of botulinum neurotoxin by the BoNT labdisk assay: Results of a trans-European performance study by Weisemann, Jasmin, Stevens, Gregory B., van Oordt, Thomas, Krez, Nadja, Dormann, Katharina, Karle, Marc, von Stetten, Felix, Rummel, Andreas

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Super-resolving botulinum neurotoxin type A molecules: from surface landing to internalization in synaptic vesicles by Joensuu, Merja, Blum, Ailisa, Mollazade, Mahdie, Lanoue, Vanessa, Padmanabhan, Pranesh, Small, Christopher, Mahrhold, Stefan, Krez, Nadja, Rummel, Andreas, Meunier, Frederic A.

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