In vitro antioxidant and cytotoxic activity of nanostructured formulations containing Ascorbyl Palmitate

In vitro antioxidant and cytotoxic activity of nanostructured formulations containing Ascorbyl Palmitate

Nanoemulsions loaded with Ascorbyl Palmitate were prepared and incorporated into Carbopol® Ultrez hydrogels. The nanoemulsions presented diameter (200 nm ± 4.02), polydispersion index (0.186 ± 0.021, zeta potential (−12 mV ± 2.14), pH (3.61 ± 0.02), spherical morphology and active content (1 mg/mL)....

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Bibliographic Details
Published in:Journal of drug delivery science and technology Vol. 86; p. 104679
Main Authors: Krawczak, Katianne Wolf, Viana, Altevir Rossato, Gundel, André, Krause, Luciana Fontanari, Ourique, Aline Ferreira
Format: Journal Article
Language:English
Published: Elsevier B.V 01-09-2023
Subjects:
Antiradical activity
Cell culture
Cytotoxicity
Hydrogels
Nanotechnology
Cell culture
Cytotoxicity
Antiradical activity
Hydrogels
Nanotechnology
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Summary:Nanoemulsions loaded with Ascorbyl Palmitate were prepared and incorporated into Carbopol® Ultrez hydrogels. The nanoemulsions presented diameter (200 nm ± 4.02), polydispersion index (0.186 ± 0.021, zeta potential (−12 mV ± 2.14), pH (3.61 ± 0.02), spherical morphology and active content (1 mg/mL). Cytotoxicity tests showed that the decrease in cell viability (VERO cells and 293T Fibroblasts) was dose-dependent. The hydrogels showed satisfactory organoleptic characteristics, diameter (226 nm ± 8.21), polydispersity index (0 0.2 ± 0.01), pH (3.5 ± 0.26), spreadability (3.96 ± 0.13 mm2.g−1) and viscosity (9253 ± 2130 mPa.s). Antioxidant activity tests showed that the hydrogels kept under refrigeration had their antioxidant activity preserved for 30 days (10 μMol TE/μL (DPPH) and 10.4 μMol TE/μL (ABTS)), while the nanoemulsions showed a reduction (values lower than 5 μMol TE μL) after 30 days. The hydrogels maintained the antioxidant activity of Ascorbyl Palmitate. [Display omitted]
ISSN:1773-2247
DOI:10.1016/j.jddst.2023.104679