Search Results - "Kravchuk, Z I"

Two high-affinity monoclonal IgG2a antibodies with differing thermodynamic stability demonstrate distinct antigen-induced changes in protein A-binding affinity by Kravchuk, Zinaida I., Chumanevich, Alexander A., Vlasov, Alexander P., Martsev, Sergey P.

“…Two IgG2a monoclonal antibodies (G10 and F11) are described which have similar affinity for human spleen ferritin and identical protein A-binding affinity. The…”
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Antigen-binding activity of monoclonal antibodies after incubation with organic solvents by Melnikova, Y I, Odintsov, S G, Kravchuk, Z I, Martsev, S P

“…Effects of four organic solvents--methanol, trifluoroethanol, dimethylsulfoxide, and dimethylformamide (DMF)--on the ferritin-binding activity of three…”
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Expression, refolding, and ferritin-binding activity of the isolated VL-domain of monoclonal antibody F11 by Dubnovitsky, A P, Kravchuk, Z I, Chumanevich, A A, Cozzi, A, Arosio, P, Martsev, S P

“…Expression of the VL-domain of mouse monoclonal antibody F11 to human spleen ferritin in Escherichia coli cells is associated with the formation of insoluble…”
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Local conformational changes in ferritins with variable iron content by Kravchuk, Z I, Konopelko, N F, Yakutovich, M D, Konoplia, E F, Martsev, S P

“…Conformational changes were induced in human spleen ferritin by partial or complete removal of iron, and the immunoreactivity of the ferritin samples with…”
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Modification of monoclonal and polyclonal IgG with palladium (II) coproporphyrin I: stimulatory and inhibitory functional effects induced by two different methods by Martsev, Sergey P., Preygerzon, Valery A., Mel'nikova, Yanina I., Kravchuk, Zinaida I., Ponomarev, Gely V., Lunev, Vitaly E., Savitsky, Alexander P.

“…Antibodies conjugated with porphyrins and metalloporphyrins have a great potential for applications in fluorescence or phosphorescence immunoassays as well as…”
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Thermodynamic stability and functional activity of tumor-associated antibodies by Bliznukov, O P, Kozmin, L D, Klimovich, V B, Tsybovsky, Y I, Kravchuk, Z I, Martsev, S P

“…Tumor-associated antibodies of human IgG1 subclass were eluted from cell-surface antigens of human carcinoma cells and studied by differential scanning…”
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Functional activation of antibodies on modification with Pd(II) coproporphyrin I N-Hydroxysuccinimide ester by Mel'nikova YaI, Kravchuk, Z I, Preygerzon, V A, Martsev, S P

“…Metalloporphyrin-antibody conjugates provide a significant advantage over other types of conjugates in biomedical use for phosphorescence immunoassay, targeted…”
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Thermodynamic stability of immunoglobulins and allosteric interactions with ferritin and protein A: distinct properties of the two antibodies of IgG2a subclass by Chumanevich, A A, Kravchuk, Z I, Vlasov, A P, Zhorov, O V, Martsev, S P

“…The two anti-ferritin monoclonal antibodies of mouse IgG2a subclass, G10 and F11, are described that have similar affinity to human spleen ferritin and…”
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Fusion of the antiferritin antibody VL domain to barnase results in enhanced solubility and altered pH stability by Martsev, Sergey P., Tsybovsky, Yaroslav I., Stremovskiy, Oleg A., Odintsov, Sergey G., Balandin, Taras G., Arosio, Paolo, Kravchuk, Zinaida I., Deyev, Sergey M.

“…Chimeric immunotoxins that combine antigen recognition domains of antibodies and cytotoxic RNases have attracted much attention in recent years as potential…”
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Antiferritin single-chain antibody: a functional protein with incomplete folding? by Martsev, Sergey P., Kravchuk, Zinaida I., Chumanevich, Alexander A., Vlasov, Alexander P., Dubnovitsky, Anatoly P., Bespalov, Ivan A., Arosio, Paolo, Deyev, Sergey M.

“…The pET(scF11) plasmid was constructed comprising the gene of a single-chain antibody against human ferritin. This plasmid encodes the leader peptide pelB…”
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Thermodynamic and functional characterization of a stable IgG conformer obtained by renaturation from a partially structured low pH-induced state by Martsev, Sergey P., Kravchuk, Zinaida I., Vlasov, Alexander P., Lyakhnovich, Georgy V.

“…At pH 2, rabbit IgG adopts a partially structured state that exhibits loss of thermal unfolding transition, tentatively assigned to the CH2 domain, whilst…”
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Large increase in thermal stability of the CH2 domain of rabbit IgG after acid treatment as evidenced by differential scanning calorimetry by Martsev, S P, Kravchuk, Z I, Vlasov, A P

“…Rabbit IgG after exposure to 0.05 M glycine-HCl, pH 2.0, and native IgG were compared by differential scanning calorimetry (DSC) at pH 3.5 and C1q binding…”
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Antiferritin single-chain Fv fragment is a functional protein with properties of a partially structured state: comparison with the completely folded V(L) domain by Martsev, S P, Chumanevich, A A, Vlasov, A P, Dubnovitsky, A P, Tsybovsky, Y I, Deyev, S M, Cozzi, A, Arosio, P, Kravchuk, Z I

“…Differential scanning calorimetry and spectroscopic probes were applied to study folding and stability of the single-chain Fv fragment (scFv) of the anti-human…”
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Non-native conformational states of immunoglobulins: thermodynamic and functional analysis of rabbit IgG by Vlasov, A P, Kravchuk, Z I, Martsev, S P

“…By changing pH within the pH range 2-7, the processes of intramolecular rearrangement in the multidomain structure of rabbit IgG were induced which resulted in…”
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Antiferritin Single-Chain Fv Fragment Is a Functional Protein with Properties of a Partially Structured State:  Comparison with the Completely Folded V L Domain by Martsev, Sergey P., Chumanevich, Alexander A., Vlasov, Alexander P., Dubnovitsky, Anatoly P., Tsybovsky, Yaroslav I., Deyev, Sergey M., Cozzi, Anna, Arosio, Paolo, Kravchuk, Zinaida I.

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Significance of serum immunoglobulin G for leukocytosis and prognosis in childhood B-lineage acute lymphoblastic leukemia by Potapnev, Michael P., Belevtsev, Michael V., Bortkevich, Ludmila G., Grinev, Vasily V., Martsev, Sergey P., Kravchuk, Zinaida I., Migal, Natalia V., Aleinikova, Olga V.

“…Background This study was conducted to evaluate the significance of serum level of immunoglobulins (Igs) and particularly IgG for leukemic cell persistence in…”
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Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11 by Martsev, Sergey P, Dubnovitsky, Anatoly P, Stremovsky, Oleg A, Chumanevich, Alexander A, Tsybovsky, Yaroslav I, Kravchuk, Zinaida I, Deyev, Sergey M

“…An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of…”
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A stable conformer of IgG, prepared by an acidic influence: study by calorimetry, binding of the C1q complement component, and monospecific anti-IgG by Kravchuk, Z I, Vlasov, A P, Liakhnovich, G V, Martsev, S P

“…Thermal stability and functional activity of rabbit IgG in its native conformation and after incubation at pH 2.0 were studied using differential scanning…”
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Interaction of rabbit IgG with anti-IgG: localization of epitopes and absence of immunoreactivity of the pFc'-fragment by Kravchuk, Z I, Zhorov, O V, Preĭgerzon, V A, Zakharova, N A, Shakhanina, K L, Martsev, S P

“…To localize essential epitopes of rabbit IgG, a series of proteolytic IgG fragments obtained by papain (Fab, Fc) or pepsin (pFc', F(ab')2) proteolysis have…”
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Thermodynamic and functional characterization of a stable IgG conformer obtained by renaturation from a partially structured low pH-induced state by Martsev, S P, Kravchuk, ZI, Vlasov, AP, Lyakhnovich, G V

“…At pH 2, rabbit IgG adopts a partially structure state that exhibits loss of thermal unfolding transition, tentatively assigned to the CH2 domain, whilst…”
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