β-Endorphin-like Peptide SLTCLVKGFY Is a Selective Agonist of Nonopioid β-Endorphin Receptor

It has been found that β-endorphin (β-END) and a synthetic β-END-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (termed immunorphin, IMN) corresponding to the sequence 364–373 of human IgG heavy chain stimulate Con A-induced proliferation of T lymphocytes from the blood of healthy donors....

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 292; no. 4; pp. 799 - 804
Main Authors:	Navolotskaya, E.V., Zargarova, T.A., Malkova, N.V., Krasnova, S.B., Zav'yalov, V.P., Lipkin, V.M.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 12-04-2002
Subjects:	Amino Acid Sequence Analgesics, Non-Narcotic - agonists beta-Endorphin - pharmacology Binding, Competitive - drug effects Binding, Competitive - physiology Cell Division - drug effects Concanavalin A - pharmacology Humans Immunoglobulin Constant Regions immunoglobulin G Immunoglobulin gamma-Chains Iodine Radioisotopes - chemistry Molecular Sequence Data naloxone-insensitive binding site Oligopeptides - chemistry Oligopeptides - metabolism Oligopeptides - pharmacology Peptide Fragments - chemistry Peptide Fragments - metabolism Peptide Fragments - pharmacology Receptors, Opioid - agonists Receptors, Opioid - metabolism Sequence Homology, Amino Acid T lymphocyte T-Lymphocytes - cytology T-Lymphocytes - drug effects T-Lymphocytes - metabolism β-endorphin naloxone-insensitive binding site immunoglobulin G β-endorphin T lymphocyte
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Summary:	It has been found that β-endorphin (β-END) and a synthetic β-END-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (termed immunorphin, IMN) corresponding to the sequence 364–373 of human IgG heavy chain stimulate Con A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met5]enkephalin ([Met5]ENK) and an antagonist of opioid receptors naloxone (NAL) tested in parallel were not active. The stimulating effect of β-END and IMN on T lymphocyte proliferation was not inhibited by NAL. Studies on receptor binding of 125I-labeled IMN to T lymphocytes revealed that it binds with high affinity to NAL-insensitive binding sites (Kd = 7.0 ± 0.3 nM). Unlabeled β-END completely inhibited the specific binding of 125I-labeled IMN to NAL-insensitive binding sites on T lymphocytes (Ki = 1.1 ± 0.2 nM). Thus, β-END and IMN bind to common NAL-insensitive binding sites on T lymphocytes and enhance Con A-induced proliferation of these cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.2002.6758