Search Results - "Kralik, Petr"

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything by Kralik, Petr, Ricchi, Matteo

“…Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the…”
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A viability assay combining palladium compound treatment with quantitative PCR to detect viable Mycobacterium avium subsp. paratuberculosis cells by Cechova, Martina, Beinhauerova, Monika, Babak, Vladimir, Kralik, Petr

“…Mycobacterium avium subsp . paratuberculosis (MAP) is a pathogenic bacterium causing the paratuberculosis, chronic and infectious disease common particularly…”
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On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination by Slana, Iva, Kralik, Petr, Kralova, Alena, Pavlik, Ivo

“…A rapid, cheap and sensitive detection method of Mycobacterium avium subsp. paratuberculosis (MAP) in raw milk was needed for routine usage. We developed two…”
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Efficiency of DNA Isolation Methods Based on Silica Columns and Magnetic Separation Tested for the Detection of Mycobacterium avium Subsp. Paratuberculosis in Milk and Faeces by Husakova, Marketa, Kralik, Petr, Babak, Vladimir, Slana, Iva

“…Timely and reliable detection of animals shedding Mycobacterium avium subsp. paratuberculosis (MAP) should help to effectively identify infected animals and…”
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Development of a reference standard for the detection and quantification of Mycobacterium avium subsp. paratuberculosis by quantitative PCR by Beinhauerova, Monika, Beinhauerova, Martina, McCallum, Sarah, Sellal, Eric, Ricchi, Matteo, O’Brien, Rory, Blanchard, Beatrice, Slana, Iva, Babak, Vladimir, Kralik, Petr

“…Quantitative PCR (qPCR) has become a frequently employed direct method for the detection and quantification of Mycobacterium avium subsp. paratuberculosis…”
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A Novel Approach to the Viability Determination of Mycobacterium avium subsp. paratuberculosis Using Platinum Compounds in Combination With Quantitative PCR by Cechova, Martina, Beinhauerova, Monika, Babak, Vladimir, Slana, Iva, Kralik, Petr

“…Mycobacterium avium subsp. (MAP) represents a slow-growing bacterium causing paratuberculosis, especially in domestic and wild ruminants. Until recently, the…”
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Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection by Vymazalová, Kateřina, Šerý, Omar, Králík, Petr, Dziedzinská, Radka, Musilová, Zuzana, Frišhons, Jan, Vojtíšek, Tomáš, Joukal, Marek

“…With the onset of the COVID-19 pandemic, a problem arose with classic body donation programmes for obtaining cadavers for anatomical dissections, science and…”
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Hepatitis E virus in pork production chain in Czech Republic, Italy, and Spain, 2010 by Di Bartolo, Ilaria, Diez-Valcarce, Marta, Vasickova, Petra, Kralik, Petr, Hernandez, Marta, Angeloni, Giorgia, Ostanello, Fabio, Bouwknegt, Martijn, Rodríguez-Lázaro, David, Pavlik, Ivo, Ruggeri, Franco Maria

“…We evaluated the prevalence of hepatitis E virus (HEV) in the pork production chain in Czech Republic, Italy, and Spain during 2010. A total of 337 fecal,…”
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Author Correction: One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles by Mikel, Pavel, Vasickova, Petra, Kralik, Petr

“…A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper…”
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The Impact of the Antimicrobial Compounds Produced by Lactic Acid Bacteria on the Growth Performance of Mycobacterium avium subsp. paratuberculosis by Kralik, Petr, Babak, Vladimir, Dziedzinska, Radka

“…Cell-free supernatants (CFSs) extracted from various lactic acid bacteria (LAB) cultures were applied to subsp. (MAP) cells to determine their effect on MAP…”
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Author Correction: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay by Reslova, Nikol, Huvarova, Veronika, Hrdy, Jakub, Kasny, Martin, Kralik, petr

“…A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper…”
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Six genetically linked mutations in the CD36 gene significantly delay the onset of Alzheimer's disease by Šerý, Omar, Zeman, Tomáš, Sheardová, Kateřina, Vyhnálek, Martin, Marková, Hana, Laczó, Jan, Lochman, Jan, Kralik, Petr, Vrzalová, Kamila, Dziedzinska, Radka, Balcar, Vladimir J., Hort, Jakub

“…The risk of Alzheimer’s disease (AD) has a strong genetic component, also in the case of late-onset AD (LOAD). Attempts to sequence whole genome in large…”
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A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay by Reslova, Nikol, Huvarova, Veronika, Hrdy, Jakub, Kasny, Martin, Kralik, Petr

“…Multiplex oligonucleotide ligation-PCR (MOL-PCR) is a rapid method for simultaneous detection of multiple molecular markers within a single reaction. MOL-PCR…”
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Occurrence of SARS-CoV-2 in Indoor Environments With Increased Circulation and Gathering of People by Dziedzinska, Radka, Kralik, Petr, Šerý, Omar

“…At the time of sampling (2020/2021), the number of new cases of SARS-CoV-2-positive individuals in the Czech Republic significantly exceeded the numbers in…”
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Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices by Mikel, Pavel, Vasickova, Petra, Tesarik, Radek, Malenovska, Hana, Kulich, Pavel, Vesely, Tomas, Kralik, Petr

“…The detection and quantification of enteric RNA viruses is based on isolation of viral RNA from the sample followed by quantitative reverse transcription…”
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The water environment as a source of potentially pathogenic mycobacteria by Makovcova, Jitka, Slany, Michal, Babak, Vladimir, Slana, Iva, Kralik, Petr

“…Nontuberculous mycobacteria (NTM) are ubiquitous organisms of a wide variety of environmental reservoirs, including natural and municipal water, soil,…”
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“Mycobacterium avium subsp. hominissuis” in Neck Lymph Nodes of Children and their Environment Examined by Culture and Triplex Quantitative Real-Time PCR by KAEVSKA, Marija, SLANA, Iva, KRALIK, Petr, REISCHL, Udo, OROSOVA, Jaroslava, HOLCIKOVA, Alena, PAVLIK, Ivo

“…Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR by Beinhauerova, Martina, Babak, Vladimir, Bertasi, Barbara, Boniotti, Maria Beatrice, Kralik, Petr

“…Quantitative PCR (qPCR) is a widely used method for nucleic acid quantification of various pathogenic microorganisms. For absolute quantification of microbial…”
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Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative real-time PCR, culture on solid media and optical densitometry by Kralik, Petr, Beran, Vladimir, Pavlik, Ivo

“…Different approaches are used for determining the number of Mycobacterium avium subsp. paratuberculosis (MAP) cells in a suspension. The majority of them are…”
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Identification of Gadoid Species in Fish Meat by Polymerase Chain Reaction (PCR) on Genomic DNA by Hubalkova, Zora, Kralik, Petr, Kasalova, Janka, Rencova, Eva

“…Identification of fish species is significant due to the increasing interest of consumers in the meat of sea fish. Methods focusing on fish species…”
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