Search Results - "Krainer, Florian W"

An updated view on horseradish peroxidases: recombinant production and biotechnological applications by Krainer, Florian W., Glieder, Anton

“…Horseradish peroxidase has been the subject of scientific research for centuries. It has been used exhaustively as reporter enzyme in diagnostics and…”
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Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway by Krainer, Florian W, Dietzsch, Christian, Hajek, Tanja, Herwig, Christoph, Spadiut, Oliver, Glieder, Anton

“…ΒACKGROUND: The methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production and is able to use methanol as a…”
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Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris by Krainer, Florian W., Gmeiner, Christoph, Neutsch, Lukas, Windwarder, Markus, Pletzenauer, Robert, Herwig, Christoph, Altmann, Friedrich, Glieder, Anton, Spadiut, Oliver

“…The yeast Pichia pastoris is a common host for the recombinant production of biopharmaceuticals, capable of performing posttranslational modifications like…”
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Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris by Krainer, Florian W, Capone, Simona, Jäger, Martin, Vogl, Thomas, Gerstmann, Michaela, Glieder, Anton, Herwig, Christoph, Spadiut, Oliver

“…Insufficient incorporation of heme is considered a central impeding cause in the recombinant production of active heme proteins. Currently, two approaches are…”
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Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris by Krainer, Florian W., Gerstmann, Michaela A., Darnhofer, Barbara, Birner-Gruenberger, Ruth, Glieder, Anton

“…•Strategies are systematically evaluated to enhance recombinant peroxidase titers.•HAC1 coexpression improves HRP production in P. pastoris and shortens…”
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Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris by Krainer, Florian W., Pletzenauer, Robert, Rossetti, Laura, Herwig, Christoph, Glieder, Anton, Spadiut, Oliver

“…•A novel 2-step purification for recombinant HRP from P. pastrois was developed.•Both purification steps are negative chromatography steps.•The amount of…”
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Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris by Vogl, Thomas, Ahmad, Mudassar, Krainer, Florian W, Schwab, Helmut, Glieder, Anton

“…Tagging proteins is a standard method facilitating protein detection, purification or targeting. When tagging a certain protein of interest, it is challenging…”
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Peroxidase gene discovery from the horseradish transcriptome by Näätsaari, Laura, Krainer, Florian W, Schubert, Michael, Glieder, Anton, Thallinger, Gerhard G

“…Horseradish peroxidases (HRPs) from Armoracia rusticana have long been utilized as reporters in various diagnostic assays and histochemical stainings…”
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