Microhardness changes in dentine after neonatal capsaicin application

Aim To determine if desensitization of the nociceptive innervation in the dental pulp has an effect on odontoblast function in the rat. Methodology Neonatal systemic application of capsaicin was used to selectively eliminate nociceptive innervation. 12 capsaicin‐treated rats were intravitally perf...

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Bibliographic Details
Published in:	International endodontic journal Vol. 38; no. 8; pp. 570 - 574
Main Authors:	Krage, T. L., Stiefel, A., Stephan, B. M., Zimmer, S., Lambrichts, I., Raab, W. H.-M.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01-08-2005
Subjects:	Analgesics, Non-Narcotic - pharmacology Animals Animals, Newborn Calcitonin Gene-Related Peptide - analysis Capsaicin - pharmacology Dental Pulp - innervation dentin Dentin - drug effects Dentin - ultrastructure Dentistry Hardness neuronal Neurons, Afferent - drug effects Nociceptors - drug effects odontoblast Odontoblasts - drug effects pulp Rats Rats, Wistar
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Summary:	Aim To determine if desensitization of the nociceptive innervation in the dental pulp has an effect on odontoblast function in the rat. Methodology Neonatal systemic application of capsaicin was used to selectively eliminate nociceptive innervation. 12 capsaicin‐treated rats were intravitally perfused at 150 days of life with 4% formaldehyde and jaws were prepared for Vicker's microhardness (VMH) measurement. As a control, 12 rats were injected with vehicle on the 3rd day of life and intravital perfusion was carried out exactly as those used for the experimental group. Immunohistological labeling of CGRP was carried out in both groups to assure the efficiency of desensitization in the experimental group. The VMH was measured in the incisors of each animal for a quantitative analysis of dentine quality. Results Vicker's microhardness was significantly higher in the control rats compared with the capsaicin‐treated rats (P < 0.001). Conclusions Neonatal systemic application of capsaicin produces changes in the quality of dentine in the rat over time and therefore it is suggestive that selective elimination of the nociceptive innervation in pulpal tissue may effect odontoblast function.
Bibliography:	ark:/67375/WNG-2X9ZXRTW-4 istex:1BA3A106F7888147EFB69349E825419E7BA0BA3C ArticleID:IEJ1000 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0143-2885 1365-2591
DOI:	10.1111/j.1365-2591.2005.01000.x