Localization dependent sensitivity of cerebral Na,K-ATPase to irradiation induced oxidative imbalance in rats

Na,K-ATPase represents the key enzyme maintaining the ionic gradient across plasma membrane. It was documented that in directly irradiated organs the activity of this enzyme is decreased. The aim of present study was to clarify the remote effect of irradiation in mediastinal area on the activity of...

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Bibliographic Details
Published in:	Journal of physiology and pharmacology : an official journal of the Polish Physiological Society Vol. 70; no. 4
Main Authors:	Kalocayova, B, Kovacicova, I, Radosinska, J, Tothova, L, Fulop, M, Slezak, J, Vrbjar, N
Format:	Journal Article
Language:	English
Published:	Poland 01-08-2019
Subjects:	Animals Cerebellum - metabolism Cerebellum - radiation effects Cerebral Cortex - metabolism Cerebral Cortex - radiation effects Male Oxidative Stress - radiation effects Protein Subunits - metabolism Rats, Wistar Sodium-Potassium-Exchanging ATPase - metabolism X-Rays
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Summary:	Na,K-ATPase represents the key enzyme maintaining the ionic gradient across plasma membrane. It was documented that in directly irradiated organs the activity of this enzyme is decreased. The aim of present study was to clarify the remote effect of irradiation in mediastinal area on the activity of the Na,K-ATPase in selected brain regions in rats. Ionizing radiation in single dose 25 Gy induced alterations in oxidative status of blood plasma. Irradiation also decreased the activity of the Na,K-ATPase in cerebral cortex. Measurements of kinetic properties of the enzyme dependently on the concentration of energy substrate ATP or cofactor Na indicated that the lowered enzyme activity is probably a consequence of decreased number of active molecules of the enzyme, as suggested by lowered V values (by 13 - 14%). Immunoblot analysis revealed that this effect is connected namely to decreased presence of α2 and α3 subunits (by 25% and 30% respectively). Considering the current concepts about involvement of the malfunction of α2 α3 subunits in development of primary brain dysfunctions, it may be hypothesized that the lowered functionality of those subunits of Na,K-ATPase may represent a predisposition to neurodegenerative disorders after irradiation. The observed effect seems to be localization dependent as the enzyme in cerebellum resisted to irradiation.
ISSN:	1899-1505
DOI:	10.26402/jpp.2019.4.08