Search Results - "Kounosu, Asako"

Continuous-Wave and Pulsed EPR Characterization of the [2Fe−2S](Cys)3(His)1 Cluster in Rat MitoNEET by Iwasaki, Toshio, Samoilova, Rimma I, Kounosu, Asako, Ohmori, Daijiro, Dikanov, Sergei A

“…CW EPR spectra of reduced [2Fe−2S](Cys)3(His)1 clusters of mammalian mitoNEET soluble domain appear to produce features resulting from the interaction of the…”
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Analysis of covalent flavinylation using thermostable succinate dehydrogenase from Thermus thermophilus and Sulfolobus tokodaii lacking SdhE homologs by Kounosu, Asako

“…•Heterologous overproduction of T. thermophilus and S. tokodaii SdhA is possible in E. coli.•SdhA covalent flavinylation requires heat and dicarboxylic…”
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Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin by Iwasaki, Toshio, Samoilova, Rimma I., Kounosu, Asako, Dikanov, Sergei A.

“…Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type [2Fe–2S] ferredoxin (ARF) from…”
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1B-10 Transformation efficiency of a novel vector containing KP element of Drosophila melanogaster by OKUMURA Keisuke, UCHIYAMA Ryo, KOUNOSU Asako, ITOH Masanobu

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Engineering a Three-cysteine, One-histidine Ligand Environment into a New Hyperthermophilic Archaeal Rieske-type [2Fe-2S] Ferredoxin from Sulfolobus solfataricus by Kounosu, Asako, Li, Zhongrui, Cosper, Nathaniel J., Shokes, Jacob E., Scott, Robert A., Imai, Takeo, Urushiyama, Akio, Iwasaki, Toshio

“…We heterologously overproduced a hyperthermostable archaeal low potential (Em = -62 mV) Rieske-type ferredoxin (ARF) from Sulfolobus solfataricus strain P-1…”
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Characterization of the pH-Dependent Resonance Raman Transitions of Archaeal and Bacterial Rieske [2Fe−2S] Proteins by Iwasaki, Toshio, Kounosu, Asako, Kolling, Derrick R. J, Crofts, Antony R, Dikanov, Sergei A, Jin, Akihisa, Imai, Takeo, Urushiyama, Akio

“…The pH-dependent resonance Raman (RR) spectral changes of the cytochrome bc 1-associated, high-potential Rieske proteins have frequently been invoked to…”
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15N HYSCORE Characterization of the Fully Deprotonated, Reduced Form of the Archaeal Rieske [2Fe−2S] Center by Iwasaki, Toshio, Kounosu, Asako, Samoilova, Rimma I, Dikanov, Sergei A

“…The hyperfine couplings for strongly and weakly coupled 15N nuclei around a reduced Rieske [2Fe−2S] center of uniformly 15N-labeled, hyperthermostable archaeal…”
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Rational Design of a Mononuclear Metal Site into the Archaeal Rieske-type Protein Scaffold by Iwasaki, Toshio, Kounosu, Asako, Tao, Ye, Li, Zhongrui, Shokes, Jacob E., Cosper, Nathaniel J., Imai, Takeo, Urushiyama, Akio, Scott, Robert A.

“…Proteins containing Rieske-type [2Fe-2S] clusters play essential functions in all three domains of life. We engineered the two histidine ligands to the…”
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Orientation-Selected 15N-HYSCORE Detection of Weakly Coupled Nitrogens around the Archaeal Rieske [2Fe−2S] Center by Iwasaki, Toshio, Kounosu, Asako, Uzawa, Taketoshi, Samoilova, Rimma I, Dikanov, Sergei A

“…The weakly coupled 15N atoms around a reduced Rieske [2Fe−2S] cluster of the uniformly 15N-labeled, hyperthermostable archaeal Rieske protein appear to produce…”
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Novel [2Fe-2S]-type Redox Center C in SdhC of Archaeal Respiratory Complex II from Sulfolobus tokodaii Strain 7 by Iwasaki, Toshio, Kounosu, Asako, Aoshima, Miho, Ohmori, Daijiro, Imai, Takeo, Urushiyama, Akio, Cosper, Nathaniel J, Scott, Robert A

“…The SdhC subunit of the archaeal respiratory complex II (succinate:quinone oxidoreductase) from Sulfolobus tokodaii strain 7 has a novel cysteine rich motif…”
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A comparative, two-dimensional 14N ESEEM characterization of reduced [2Fe-2S] clusters in hyperthermophilic archaeal high- and low-potential Rieske-type proteins by Dikanov, Sergei A, Shubin, Alexandr A, Kounosu, Asako, Iwasaki, Toshio, Samoilova, Rimma I

“…Proteins of the Rieske and Rieske-type family contain a [2Fe-2S] cluster with mixed ligation by two histidines and two cysteines, and play important roles in…”
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Two‐dimensional pulsed electron spin resonance characterization of 15 N‐labeled archaeal Rieske‐type ferredoxin by Iwasaki, Toshio, Samoilova, Rimma I., Kounosu, Asako, Dikanov, Sergei A.

“…Two‐dimensional electron spin‐echo envelope modulation (ESEEM) analysis of the uniformly 15 N‐labeled archaeal Rieske‐type [2Fe–2S] ferredoxin (ARF) from…”
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15 N HYSCORE Characterization of the Fully Deprotonated, Reduced Form of the Archaeal Rieske [2Fe−2S] Center by Iwasaki, Toshio, Kounosu, Asako, Samoilova, Rimma I., Dikanov, Sergei A.

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Crystallization and preliminary X-ray diffraction studies of hyperthermophilic archaeal Rieske-type ferredoxin (ARF) from Sulfolobus solfataricus P1 by Kounosu, Asako, Hasegawa, Kazuya, Iwasaki, Toshio, Kumasaka, Takashi

“…The hyperthermophilic archaeal Rieske‐type [2Fe–2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 contains a low‐potential Rieske‐type [2Fe–2S] cluster that…”
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Orientation-Selected 15 N-HYSCORE Detection of Weakly Coupled Nitrogens around the Archaeal Rieske [2Fe−2S] Center by Iwasaki, Toshio, Kounosu, Asako, Uzawa, Taketoshi, Samoilova, Rimma I., Dikanov, Sergei A.

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Resonance Raman characterization of archaeal and bacterial Rieske protein variants with modified hydrogen bond network around the [2Fe‐2S] center by Iwasaki, Toshio, Kounosu, Asako, Kolling, Derrick R.J., Lhee, Sangmoon, Crofts, Antony R., Dikanov, Sergei A., Uchiyama, Takuro, Kumasaka, Takashi, Ishikawa, Hiroyuki, Kono, Miwa, Imai, Takeo, Urushiyama, Akio

“…The rate of quinol oxidation by cytochrome bc1/b6f complex is in part associated with the redox potential (Em) of its Rieske [2Fe‐2S] center, for which an…”
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Crystallization and preliminary X-ray diffraction studies of the prototypal homologue of mitoNEET (Tth-NEET0026) from the extreme thermophile Thermus thermophilus HB8 by Kounosu, Asako, Iwasaki, Toshio, Baba, Seiki, Hayashi-Iwasaki, Yoko, Oshima, Tairo, Kumasaka, Takashi

“…MitoNEET (a mammalian mitochondrial outer membrane protein) is a potential pharmacological and clinical target of the insulin‐sensitizer pioglitazone. The…”
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Redox‐dependent structural changes in archaeal and bacterial Rieske‐type [2Fe‐2S] clusters by Cosper, Nathaniel J., Eby, D. Matthew, Kounosu, Asako, Kurosawa, Norio, Neidle, Ellen L., Kurtz, Donald M., Iwasaki, Toshio, Scott, Robert A.

“…Proteins containing Rieske‐type [2Fe‐2S] clusters play important roles in many biological electron transfer reactions. Typically, [2Fe‐2S] clusters are not…”
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15N hyscore characterization of the fully deprotonated, reduced form of the archaeal rieske [2Fe-2S] center by IWASAKI, Toshio, KOUNOSU, Asako, SAMOILOVA, Rimma I, DIKANOV, Sergei A

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Crystallization and preliminary X-ray diffraction studies of the hyperthermophilic archaeal sulredoxin having the unique Rieske [2Fe-2S] cluster environment by Uchiyama, Takuro, Kounosu, Asako, Sato, Takao, Tanaka, Nobuo, Iwasaki, Toshio, Kumasaka, Takashi

“…The hyperthermophilic archaeal sulredoxin from Sulfolobus tokodaii is a water‐soluble high‐potential Rieske [2Fe–2S] protein with unique pH‐dependent redox…”
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