Search Results - "Kouhei Tsumoto"

Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibody by Matsunaga, Ryo, Tsumoto, Kouhei

“…Brevibacillus choshinensis is a gram-positive bacterium that is known to efficiently secrete recombinant proteins. However, the expression of these proteins is…”
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Effects of syringe material and silicone oil lubrication on the stability of pharmaceutical proteins by Krayukhina, Elena, Tsumoto, Kouhei, Uchiyama, Susumu, Fukui, Kiichi

“…Currently, polymer-based prefillable syringes are being promoted to the pharmaceutical market because they provide an increased break resistance relative to…”
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Analytical Method for Experimental Validation of Computer-Designed Antibody by Tanabe, Aki, Tsumoto, Kouhei

“…In the computational design of antibodies, the interaction analysis between target antigen and antibody is an essential process to obtain feedback for…”
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Effects of subclass change on the structural stability of chimeric, humanized, and human antibodies under thermal stress by Ito, Takahiko, Tsumoto, Kouhei

“…To address how changes in the subclass of antibody molecules affect their thermodynamic stability, we prepared three types of four monoclonal antibody…”
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Development of novel humanized VHH synthetic libraries based on physicochemical analyses by Nakakido, Makoto, Kinoshita, Seisho, Tsumoto, Kouhei

“…Due to the high affinity and specificity of antibodies toward antigens, various antibody-based applications have been developed. Recently, variable…”
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Thermodynamic and molecular dynamic insights into how fusion influences peptide-tag recognition of an antibody by Miyanabe, Kazuhiro, Yamashita, Takefumi, Tsumoto, Kouhei

“…To understand the effect of protein fusion on the recognition of a peptide-tag by an antibody, we fused a CCR5-derived peptide-tag (pep1) to GFP and…”
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Thermodynamics of antibody-antigen interaction revealed by mutation analysis of antibody variable regions by Akiba, Hiroki, Tsumoto, Kouhei

“…Antibodies (immunoglobulins) bind specific molecules (i.e. antigens) with high affinity and specificity. In order to understand their mechanisms of…”
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Glycosylation of IgG-Fc: a molecular perspective by Kiyoshi, Masato, Tsumoto, Kouhei, Ishii-Watabe, Akiko, Caaveiro, Jose M M

“…Antibodies of the IgG class carry a pair of oligosaccharides (N-glycans) in the Fc region. The importance of the N-glycan is clearly demonstrated by its…”
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Next generation drying technologies for pharmaceutical applications by Walters, Robert H, Bhatnagar, Bakul, Tchessalov, Serguei, Izutsu, Ken-Ichi, Tsumoto, Kouhei, Ohtake, Satoshi

“…Drying is a commonly used technique for improving the product stability of biotherapeutics. Typically, drying is accomplished through freeze-drying, as…”
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How the protonation state of a phosphorylated amino acid governs molecular recognition: insights from classical molecular dynamics simulations by Kawade, Raiji, Kuroda, Daisuke, Tsumoto, Kouhei

“…Physicochemical properties of proteins are controlled mainly by post‐translational modifications such as amino acid phosphorylation. Although molecular…”
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Discovery of chemical probes that suppress Wnt/β‐catenin signaling through high‐throughput screening by Yamaguchi, Kiyoshi, Nagatoishi, Satoru, Tsumoto, Kouhei, Furukawa, Yoichi

“…Aberrant activation of the Wnt/β‐catenin signaling pathway has been observed in a wide range of human tumors. Deregulation of the pathway is closely linked to…”
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Thermodynamic and computational analyses reveal the functional roles of the galloyl group of tea catechins in molecular recognition by Takahashi, Tomoya, Nagatoishi, Satoru, Kuroda, Daisuke, Tsumoto, Kouhei

“…Catechins, biologically active polyphenols in green tea, exhibit various biological activities, such as anticancer and antiviral activities, arising from…”
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Development of a high-throughput method to screen novel antiviral materials by Nakakido, Makoto, Tanaka, Naoki, Shimojo, Ayako, Miyamae, Nobuhiro, Tsumoto, Kouhei

“…Respiratory infectious diseases pose a serious threat worldwide, and novel antiviral materials are highly demanded. Photocatalytic nanoparticles have been…”
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Delicate balance among thermal stability, binding affinity, and conformational space explored by single-domain VHH antibodies by Ikeuchi, Emina, Kuroda, Daisuke, Nakakido, Makoto, Murakami, Akikazu, Tsumoto, Kouhei

“…The high binding affinities and specificities of antibodies have led to their use as drugs and biosensors. Single-domain V H H antibodies exhibit high…”
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Structural basis for self-assembly of a cytolytic pore lined by protein and lipid by Tanaka, Koji, Caaveiro, Jose M.M., Morante, Koldo, González-Mañas, Juan Manuel, Tsumoto, Kouhei

“…Pore-forming toxins (PFT) are water-soluble proteins that possess the remarkable ability to self-assemble on the membrane of target cells, where they form…”
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Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores by Watanabe, Hirokazu, Gubbiotti, Alberto, Chinappi, Mauro, Takai, Natsumi, Tanaka, Koji, Tsumoto, Kouhei, Kawano, Ryuji

“…This paper describes the analysis of pore formation and detection of a single protein molecule using a large nanopore among five different pore-forming…”
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Microsecond molecular dynamics suggest that a non-synonymous mutation, frequently observed in patients with mild symptoms in Tokyo, alters dynamics of the SARS-CoV-2 main protease by Kuroda, Daisuke, Tsumoto, Kouhei

“…Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes the coronavirus disease 2019 (COVID-19), spread rapidly around the globe. The main…”
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Exploring designability of electrostatic complementarity at an antigen-antibody interface directed by mutagenesis, biophysical analysis, and molecular dynamics simulations by Yoshida, Kouhei, Kuroda, Daisuke, Kiyoshi, Masato, Nakakido, Makoto, Nagatoishi, Satoru, Soga, Shinji, Shirai, Hiroki, Tsumoto, Kouhei

“…Antibodies protect organisms from a huge variety of foreign antigens. Antibody diversity originates from both genetic and structural levels. Antigen…”
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The transcriptional corepressor CtBP2 serves as a metabolite sensor orchestrating hepatic glucose and lipid homeostasis by Sekiya, Motohiro, Kainoh, Kenta, Sugasawa, Takehito, Yoshino, Ryunosuke, Hirokawa, Takatsugu, Tokiwa, Hiroaki, Nakano, Shogo, Nagatoishi, Satoru, Tsumoto, Kouhei, Takeuchi, Yoshinori, Miyamoto, Takafumi, Matsuzaka, Takashi, Shimano, Hitoshi

“…Biological systems to sense and respond to metabolic perturbations are critical for the maintenance of cellular homeostasis. Here we describe a hepatic system…”
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Phospholipid Membrane Fluidity Alters Ligand Binding Activity of a G Protein-Coupled Receptor by Shifting the Conformational Equilibrium by Yoshida, Kouhei, Nagatoishi, Satoru, Kuroda, Daisuke, Suzuki, Nanao, Murata, Takeshi, Tsumoto, Kouhei

“…The affinity of a ligand for a receptor on the cell surface will be influenced by the membrane composition. Herein, we evaluated the effects of differences in…”
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