takeout, a novel Drosophila gene under circadian clock transcriptional regulation

We report the identification and characterization of a new Drosophila clock-regulated gene, takeout (to). to is a member of a novel gene family and is implicated in circadian control of feeding behavior. Its gene expression is down-regulated in all of the clock mutants tested. In wild-type flies, to...

Full description

Saved in:

Bibliographic Details
Published in:	Molecular and cellular biology Vol. 20; no. 18; pp. 6935 - 6944
Main Authors:	So, W V, Sarov-Blat, L, Kotarski, C K, McDonald, M J, Allada, R, Rosbash, M
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 01-09-2000
Subjects:	Amino Acid Sequence Animals Base Sequence Biological Clocks - physiology Cell Line circadian clocks Circadian Rhythm - physiology DNA, Complementary Down-Regulation Drosophila Drosophila melanogaster - genetics Drosophila Proteins Genes, Insect Head Insect Proteins - genetics Insect Proteins - physiology Molecular Sequence Data Nuclear Proteins - genetics Period Circadian Proteins Promoter Regions, Genetic RNA, Messenger Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid takeout (to) gene Transcription, Genetic Transcriptional Regulation
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We report the identification and characterization of a new Drosophila clock-regulated gene, takeout (to). to is a member of a novel gene family and is implicated in circadian control of feeding behavior. Its gene expression is down-regulated in all of the clock mutants tested. In wild-type flies, to mRNA exhibits daily cycling expression but with a novel phase, delayed relative to those of the better-characterized clock mRNAs, period and timeless. The E-box-containing sequence in the to promoter shows impressive similarities with those of period and timeless. However, our results suggest that the E box is not involved in the amplitude and phase of the transcriptional cycling of to. The circadian delayed transcriptional phase is therefore most likely the result of indirect regulation through unknown transcription factors.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Howard Hughes Medical Institute, NSF Center for Biological Timing, Department of Biology, Brandeis University, Waltham, MA 02454. Phone: (781) 736-3161. Fax: (781) 736-3164. E-mail: rosbash@brandeis.edu. Present address: Hoffmann-La Roche, Inc., Nutley, NJ 07110.
ISSN:	0270-7306 1098-5549 1098-5549
DOI:	10.1128/MCB.20.18.6935-6944.2000