Search Results - "Kotłowski, Roman"
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Use of Escherichia coli Nissle 1917 producing recombinant colicins for treatment of IBD patients
Published in Medical hypotheses (01-08-2016)“…Abstract Patients with Crohn’s Disease and Ulcerative Colitis infected with Adherent-Invasive Escherichia coli strains constitute the largest group among…”
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2
Influence of Escherichia coli on Expression of Selected Human Drug Addiction Genes
Published in Life (Basel, Switzerland) (05-12-2021)“…The impact of enteric microflora on the expression of genes associated with cocaine and amphetamine addiction was described. Human genome-wide experiments on…”
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Linezolid-resistant Enterococcus faecium strains isolated from one hospital in Poland -commensals or hospital-adapted pathogens?
Published in PloS one (26-05-2020)“…One of the most pressing problems of enterococci infections is occurring resistance to linezolid, which is an antibiotic used in the treatment of infections…”
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A novel method of Mycobacterium tuberculosis complex strain differentiation using polymorphic GC-rich gene sequences
Published in Acta biochimica Polonica (2015)“…Tuberculosis is one of the leading infectious diseases. In this work, a new genotyping method of Mycobacterium tuberculosis (Mtb) complex strain is presented…”
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Novel staphylococci nucH taxonomical marker used in identification of human‐associated Staphylococcus succinus subsp. casei
Published in Environmental microbiology reports (01-10-2021)“…Summary The aim of our study was to assess the sequencing of unique nucH gene fragment based on performed bioinformatics analysis as a novel diagnostic method…”
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Discrimination of hospital isolates of Acinetobacter baumannii using repeated sequences and whole genome alignment differential analysis
Published in Journal of applied genetics (01-09-2021)“…An optimized method for bacterial strain differentiation, based on combination of Repeated Sequences and Whole Genome Alignment Differential Analysis…”
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Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes
Published in Acta biochimica polonica (10-01-2019)“…It is still under investigation, whether all Borrelia sp. causing Lyme borreliosis and other diseases are already identified and properly classified as human…”
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Intra-operative biopsy in chronic sinusitis detects pathogenic Escherichia coli that carry fimG/H, fyuA and agn43 genes coding biofilm formation
Published in PloS one (23-03-2018)“…The aim of this study was to investigate whether or not surgical biopsy of sinus tissue in chronic sinusitis, not responsive to treatment, would detect E…”
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New Approaches for Escherichia coli Genotyping
Published in Pathogens (Basel) (21-01-2020)“…Easy-to-perform, fast, and inexpensive methods of differentiation of strains beyond the species level are highly required. Herein two new, original tools for…”
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Correlation between the number of Pro–Ala repeats in the EmrA homologue of Acinetobacter baumannii and resistance to netilmicin, tobramycin, imipenem and ceftazidime
Published in Journal of global antimicrobial resistance. (01-12-2016)“…•CAP01997 protein is the A. baumannii homologue of the E. coli transporter EmrA.•Significant correlation between susceptibility to four antibiotics and number…”
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Novel method of expression and purification of hirudin based on pBAD TOPO, pTYB12 vectors and gene synthesis
Published in Protein expression and purification (01-11-2006)“…To express recombinant hirudins in Escherichia coli cells, a fragment of chemically synthesized DNA was used, containing codons for the individual amino acids…”
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Comparative analysis of mRNA transcripts of HT-29 cell line expressed in identical quantities for pathogenic E. coli strains UM146 and UM147 with control Escherichia coli Nissle 1917
Published in Journal of genomics (2022)“…Aim of study was comparative analysis of mRNA transcripts of HT-29 cell line, expressed in identical quantities for the combination of pathogenic and…”
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13
Evaluation of multiple genetic markers for typing drug-resistant Mycobacterium tuberculosis strains from Poland
Published in Diagnostic microbiology and infectious disease (01-05-2006)“…In the present study, 77 drug-resistant Mycobacterium tuberculosis strains isolated in Poland in 2000 were characterized by the mycobacterial interspersed…”
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14
Microbial diversity of inflamed and noninflamed gut biopsy tissues in inflammatory bowel disease
Published in Inflammatory bowel diseases (01-06-2007)“…Background: Inflammatory bowel disease (IBD) is a chronic gastrointestinal condition without any known cause or cure. An imbalance in normal gut biota has been…”
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15
Selection of primers for specific detection of Clostridium botulinum types B and E neurotoxin genes using PCR method
Published in International journal of food microbiology (28-09-2001)“…Improved oligonucleotide primers were designed to flank 370- and 307-bp fragments of the bont genes encoding botulinum neurotoxins types B and E, respectively…”
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PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers
Published in Journal of Clinical Microbiology (01-01-2004)“…Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Amplification of a single-locus variable-number direct repeats with restriction fragment length polymorphism (DR-PCR/RFLP) for genetic typing of Acinetobacter baumannii strains
Published in Polish journal of microbiology (2008)“…In search of an effective DNA typing technique for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new…”
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Protective effect of lysostaphin from Staphylococcus simulans against growth of Staphylococcus aureus in milk and some other food products
Published in Journal of food safety (01-08-2007)“…The effect of lysostaphin from Staphylococcus simulans expressed in Escherichia coli TOP10 strain on Staphylococcus aureus used for inoculation of milk, ground…”
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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms
Published in Roczniki Państwowego Zakładu Higieny (2017)“…As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there…”
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New effective sources of the Staphylococcus simulans lysostaphin
Published in Journal of biotechnology (04-05-2005)“…The gene encoding Staphylococcus simulans lysostaphin has been cloned into two Escherichia coli expression systems: pET23b(+) (Novagen, UK) and pBAD/Thio-TOPO…”
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