Search Results - "Kostyuk, D.A"

Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase by Gudima, S.O, Kostyuk, D.A, Grishchenko, O.I, Tunitskaya, V.L, Memelova, L.V, Kochetkov, S.N

“…Synthesis of deoxynucleotide-containing RNA-like single-stranded polynucleotides (dcRNAs) using the Y639F, S641A mutant of T7 RNA polymerase (T7 RNAP) was…”
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Mutant T7 RNA polymerase is capable of catalyzing DNA primer extension reaction by Rusakova, E.E, Tunitskaya, V.L, Memelova, L.V, Kochetkova, S.V, Kostyuk, D.A, Kochetkov, S.N

“…The mutant T7 RNA polymerase (T7 RNAP), containing two substitutions (Y639F, S641A) was earlier shown to utilize both rNTP and dNTP in a transcription-like…”
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Reflection and refraction of acoustic waves at the insulator-ferromagnetic heusler alloy boundary by Karpuk, M. M., Kostyuk, D. A., Kuzavko, Yu. A., Shavrov, V. G.

“…Reflection and refraction of longitudinal and transverse acoustic waves on a flat boundary between an insulator and a ferromagnetic crystal of a Heusler alloy…”
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Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA by Kostyuk, D.A., Dragan, S.M., Lyakhov, D.L., Rechinsky, V.O., Tunitskaya, V.L., Chernov, B.K., Kochetkov, S.N.

“…A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is…”
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On the functional role of the Tyr-639 residue of bacteriophage T7 RNA polymerase by Rechinsky, V.O., Kostyuk, D.A., Tunitskaya, V.L., Kochetkov, S.N.

“…Substitution or Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic…”
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Tyr-571 is involved in the T7 RNA polymerase binding to its promoter by Rechinsky, V.O., Tunitskaya, V.L., Dragan, S.M., Kostyuk, D.A., Kochetkov, S.N.

“…The in vitro studies of three T7 RNA polymerase point mutants suggest that substitutions of Ala and Thr for Pro-563 and of Ser for Tyr-571 have little effect…”
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Targeted mutagenesis identifies Asp-569 as a catalytically critical residue in T7 RNA polymerase by Rechinsky, V O, Chernov, B K, Dragan, S M, Kostyuk, D A, Tunitskaya, V L, Kochetkov, S N

“…In order to look more closely at a well-conserved region in T7 RNA polymerase (T7 RNAP) containing, as shown earlier, the functionally essential residues…”
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