Search Results - "Kossiakoff, A"

Cryo-EM structure of human rhodopsin bound to an inhibitory G protein by Kang, Yanyong, Kuybeda, Oleg, de Waal, Parker W., Mukherjee, Somnath, Van Eps, Ned, Dutka, Przemyslaw, Zhou, X. Edward, Bartesaghi, Alberto, Erramilli, Satchal, Morizumi, Takefumi, Gu, Xin, Yin, Yanting, Liu, Ping, Jiang, Yi, Meng, Xing, Zhao, Gongpu, Melcher, Karsten, Ernst, Oliver P., Kossiakoff, Anthony A., Subramaniam, Sriram, Xu, H. Eric

“…G-protein-coupled receptors comprise the largest family of mammalian transmembrane receptors. They mediate numerous cellular pathways by coupling with…”
Get full text

A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel by Feng, Peiqiang, Wu, Xudong, Erramilli, Satchal K., Paulo, Joao A., Knejski, Pawel, Gygi, Steven P., Kossiakoff, Anthony A., Rapoport, Tom A.

“…Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health 1 – 4 . Luminal peroxisomal proteins are…”
Get full text

Visualization of arrestin recruitment by a G-protein-coupled receptor by Shukla, Arun K., Westfield, Gerwin H., Xiao, Kunhong, Reis, Rosana I., Huang, Li-Yin, Tripathi-Shukla, Prachi, Qian, Jiang, Li, Sheng, Blanc, Adi, Oleskie, Austin N., Dosey, Anne M., Su, Min, Liang, Cui-Rong, Gu, Ling-Ling, Shan, Jin-Ming, Chen, Xin, Hanna, Rachel, Choi, Minjung, Yao, Xiao Jie, Klink, Bjoern U., Kahsai, Alem W., Sidhu, Sachdev S., Koide, Shohei, Penczek, Pawel A., Kossiakoff, Anthony A., Woods Jr, Virgil L., Kobilka, Brian K., Skiniotis, Georgios, Lefkowitz, Robert J.

“…Single-particle electron microscopy and hydrogen–deuterium exchange mass spectrometry are used to characterize the structure and dynamics of a…”
Get full text

Structure of human drug transporters OATP1B1 and OATP1B3 by Ciută, Anca-Denise, Nosol, Kamil, Kowal, Julia, Mukherjee, Somnath, Ramírez, Ana S., Stieger, Bruno, Kossiakoff, Anthony A., Locher, Kaspar P.

“…The organic anion transporting polypeptides OATP1B1 and OATP1B3 are membrane proteins that mediate uptake of drugs into the liver for subsequent conjugation…”
Get full text

Structure and mechanism of the ER-based glucosyltransferase ALG6 by Bloch, Joël S., Pesciullesi, Giorgio, Boilevin, Jérémy, Nosol, Kamil, Irobalieva, Rossitza N., Darbre, Tamis, Aebi, Markus, Kossiakoff, Anthony A., Reymond, Jean-Louis, Locher, Kaspar P.

“…In eukaryotic protein N -glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its…”
Get full text

Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling by Tsutsumi, Naotaka, Mukherjee, Somnath, Waghray, Deepa, Janda, Claudia Y, Jude, Kevin M, Miao, Yi, Burg, John S, Aduri, Nanda Gowtham, Kossiakoff, Anthony A, Gati, Cornelius, Garcia, K Christopher

“…Frizzleds (Fzd) are the primary receptors for Wnt morphogens, which are essential regulators of stem cell biology, yet the structural basis of Wnt signaling…”
Get full text

High-throughput Generation of Synthetic Antibodies from Highly Functional Minimalist Phage-displayed Libraries by Fellouse, Frederic A., Esaki, Kaori, Birtalan, Sara, Raptis, Demetrios, Cancasci, Vincenzo J., Koide, Akiko, Jhurani, Parkash, Vasser, Mark, Wiesmann, Christian, Kossiakoff, Anthony A., Koide, Shohei, Sidhu, Sachdev S.

“…We have previously established a minimalist approach to antibody engineering by using a phage-displayed framework to support complementarity determining region…”
Get full text

Architecture of the nuclear pore complex coat by Stuwe, Tobias, Correia, Ana R., Lin, Daniel H., Paduch, Marcin, Lu, Vincent T., Kossiakoff, Anthony A., Hoelz, André

“…The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. Despite half a century of structural…”
Get full text

Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP by Liu, Hongtao, Zakrzewicz, Dariusz, Nosol, Kamil, Irobalieva, Rossitza N., Mukherjee, Somnath, Bang-Sørensen, Rose, Goldmann, Nora, Kunz, Sebastian, Rossi, Lorenzo, Kossiakoff, Anthony A., Urban, Stephan, Glebe, Dieter, Geyer, Joachim, Locher, Kaspar P.

“…Cellular entry of the hepatitis B and D viruses (HBV/HDV) requires binding of the viral surface polypeptide preS1 to the hepatobiliary transporter Na +…”
Get full text

Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins by Mukherjee, Somnath, Erramilli, Satchal K., Ammirati, Mark, Alvarez, Frances J. D., Fennell, Kimberly F., Purdy, Michael D., Skrobek, Blazej M., Radziwon, Katarzyna, Coukos, John, Kang, Yanyong, Dutka, Przemysław, Gao, Xiang, Qiu, Xiayang, Yeager, Mark, Eric Xu, H., Han, Seungil, Kossiakoff, Anthony A.

“…We propose the concept of universal fiducials based on a set of pre-made semi-synthetic antibodies (sABs) generated by customized phage display selections…”
Get full text

Structural basis for activation of voltage sensor domains in an ion channel TPC1 by Kintzer, Alexander F., Green, Evan M., Dominik, Pawel K., Bridges, Michael, Armache, Jean-Paul, Deneka, Dawid, Kim, Sangwoo S., Hubbell, Wayne, Kossiakoff, Anthony A., Cheng, Yifan, Stroud, Robert M.

“…Voltage-sensing domains (VSDs) couple changes in transmembrane electrical potential to conformational changes that regulate ion conductance through a central…”
Get full text

Structural basis of lipopolysaccharide maturation by the O-antigen ligase by Ashraf, Khuram U., Nygaard, Rie, Vickery, Owen N., Erramilli, Satchal K., Herrera, Carmen M., McConville, Thomas H., Petrou, Vasileios I., Giacometti, Sabrina I., Dufrisne, Meagan Belcher, Nosol, Kamil, Zinkle, Allen P., Graham, Chris L. B., Loukeris, Michael, Kloss, Brian, Skorupinska-Tudek, Karolina, Swiezewska, Ewa, Roper, David I., Clarke, Oliver B., Uhlemann, Anne-Catrin, Kossiakoff, Anthony A., Trent, M. Stephen, Stansfeld, Phillip J., Mancia, Filippo

“…The outer membrane of Gram-negative bacteria has an external leaflet that is largely composed of lipopolysaccharide, which provides a selective permeation…”
Get full text

Architecture of the fungal nuclear pore inner ring complex by Stuwe, Tobias, Bley, Christopher J., Thierbach, Karsten, Petrovic, Stefan, Schilbach, Sandra, Mayo, Daniel J., Perriches, Thibaud, Rundlet, Emily J., Jeon, Young E., Collins, Leslie N., Huber, Ferdinand M., Lin, Daniel H., Paduch, Marcin, Koide, Akiko, Lu, Vincent, Fischer, Jessica, Hurt, Ed, Koide, Shohei, Kossiakoff, Anthony A., Hoelz, André

“…The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. We present the reconstitution and interdisciplinary…”
Get full text

Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers by Lim, Shion A, Zhou, Jie, Martinko, Alexander J, Wang, Yung-Hua, Filippova, Ekaterina V, Steri, Veronica, Wang, Donghui, Remesh, Soumya G, Liu, Jia, Hann, Byron, Kossiakoff, Anthony A, Evans, Michael J, Leung, Kevin K, Wells, James A

“…Extracellular proteolysis is frequently dysregulated in disease and can generate proteoforms with unique neoepitopes not found in healthy tissue. Here, we…”
Get full text

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry by Leonhardt, Susan A., Purdy, Michael D., Grover, Jonathan R., Yang, Ziwei, Poulos, Sandra, McIntire, William E., Tatham, Elizabeth A., Erramilli, Satchal K., Nosol, Kamil, Lai, Kin Kui, Ding, Shilei, Lu, Maolin, Uchil, Pradeep D., Finzi, Andrés, Rein, Alan, Kossiakoff, Anthony A., Mothes, Walther, Yeager, Mark

“…The host proteins SERINC3 and SERINC5 are HIV-1 restriction factors that reduce infectivity when incorporated into the viral envelope. The HIV-1 accessory…”
Get full text

Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder by Kordon, Szymon P., Dutka, Przemysław, Adamska, Justyna M., Bandekar, Sumit J., Leon, Katherine, Erramilli, Satchal K., Adams, Brock, Li, Jingxian, Kossiakoff, Anthony A., Araç, Demet

“…Adhesion G protein-coupled receptors (aGPCRs) are cell-surface proteins with large extracellular regions that bind to multiple ligands to regulate key…”
Get full text

Generating conformation-specific synthetic antibodies to trap proteins in selected functional states by Paduch, Marcin, Koide, Akiko, Uysal, Serdar, Rizk, Shahir S., Koide, Shohei, Kossiakoff, Anthony A.

“…[Display omitted] ► We present a streamlined method of generating synthetic antibodies by phage display. ► In vitro selections allow for unique control over…”
Get full text

Development of “Plug and Play” Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM by Dutka, Przemyslaw, Mukherjee, Somnath, Gao, Xiang, Kang, Yanyong, de Waal, Parker W., Wang, Lei, Zhuang, Youwen, Melcher, Karsten, Zhang, Cheng, Xu, H. Eric, Kossiakoff, Anthony A.

“…“Universal” synthetic antibody (sAB)-based fiducial marks have been generated by customized phage display selections to facilitate the rapid structure…”
Get full text

Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs by Dominik, Pawel K., Borowska, Marta T., Dalmas, Olivier, Kim, Sangwoo S., Perozo, Eduardo, Keenan, Robert J., Kossiakoff, Anthony A.

“…A major challenge in membrane biophysics is to define the mechanistic linkages between a protein's conformational transitions and its function. We describe a…”
Get full text

X-ray Structure of Snow Flea Antifreeze Protein Determined by Racemic Crystallization of Synthetic Protein Enantiomers by Pentelute, Brad L, Gates, Zachary P, Tereshko, Valentina, Dashnau, Jennifer L, Vanderkooi, Jane M, Kossiakoff, Anthony A, Kent, Stephen B. H

“…Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal…”
Get full text