Comparative Proteomical Analysis of Zygotic Embryo and Endosperm from Coffea arabica Seeds

Comparative Proteomical Analysis of Zygotic Embryo and Endosperm from Coffea arabica Seeds

During coffee seed development, proteins are predominantly deposited in cotyledons and in the endosperm. Reserve proteins of the 11S family are the most abundant globulins in coffee seeds, acting as a nitrogen source during roasting and guaranteeing flavor and aroma. The aim of the present study was...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry Vol. 56; no. 22; pp. 10922 - 10926
Main Authors: Koshino, Lívia L, Gomes, Clarissa P, Silva, Luciano P, Eira, Mirian T. S, Bloch Jr, Carlos, Franco, Octávio L, Mehta, Ângela
Format: Journal Article
Language:English
Published: Washington, DC American Chemical Society 26-11-2008
Subjects:
11S globulin
2-DE
Amino Acid Sequence
Biological and medical sciences
Coffea - embryology
Coffea arabica
Electrophoresis, Gel, Two-Dimensional
embryo (plant)
endosperm
Food Chemistry/Biochemistry
Food industries
Fundamental and applied biological sciences. Psychology
globulins
Mass Spectrometry
Molecular Sequence Data
plant proteins
Plant Proteins - analysis
Plant Proteins - chemistry
proteome
proteomics
Seed Storage Proteins - analysis
seeds
Seeds - chemistry
Trypsin - metabolism
zygote
zygotic embryo
zygotic embryo
mass spectrometry
2-DE
Coffea arabica
endosperm
Embryo
Seeds
Endosperm
Mass spectrometry
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Summary:During coffee seed development, proteins are predominantly deposited in cotyledons and in the endosperm. Reserve proteins of the 11S family are the most abundant globulins in coffee seeds, acting as a nitrogen source during roasting and guaranteeing flavor and aroma. The aim of the present study was to compare the protein profiles of endosperm and zygotic embryos of coffee seeds. Proteins were extracted from whole seed as well as from embryo and endosperm, separately. Total proteins were analyzed by two-dimensional electrophoresis (2-DE) followed by identification by mass spectrometry (MS). The most abundant spots observed in the gels of coffee seeds were excised, digested with trypsin, and identified by MS as subunits of the 11S globulin. Spots with identical pI and molecular masses were also observed in the protein profiles of coffee endosperm and embryo, indicating that 11S protein is also highly expressed in those tissues. Peptide sequence coverage of about 20% of the entire 11S globulin was obtained. Three other proteins were identified in the embryo and endosperm 2-DE profiles as a Cupin superfamily protein, an allergenic protein (Pru ar 1), exclusive to the endosperm 2D map, and a hypothetical protein, observed only in the zygotic embryo profile.
Bibliography:http://dx.doi.org/10.1021/jf801734m
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content type line 23
ISSN:0021-8561
1520-5118
DOI:10.1021/jf801734m