In vivo quantification and pharmacokinetic studies of cotinine in mice after smoke exposure by LC–MS/MS

In vivo quantification and pharmacokinetic studies of cotinine in mice after smoke exposure by LC–MS/MS

A sensitive analytical method was developed and validated for the quantification of cotinine in mouse plasma after exposure to smoke of 0.5, 1.0, and 1.5 commercially available cigarettes, using liquid chromatography tandem mass spectrometry. The method was validated over a linear concentration rang...

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Bibliographic Details
Published in:Biomedical chromatography Vol. 34; no. 2; pp. e4752 - n/a
Main Authors: El Mubarak, Mohamed A., Danika, Charikleia, Cachon, Charlyne, Korovila, Charalambia, Atsopardi, Korina, Panagopoulos, Nikolaos, Margarity, Marigoula, Poulas, Konstantinos, Sivolapenko, Gregory B.
Format: Journal Article
Language:English
Published: England 01-02-2020
Subjects:
Animals
cotinine
Cotinine - blood
Cotinine - chemistry
Cotinine - pharmacokinetics
Inhalation Exposure - analysis
LC–MS/MS
Linear Models
Male
method validation
Mice
Mice, Inbred BALB C
mouse plasma
Reproducibility of Results
Sensitivity and Specificity
Tobacco Smoke Pollution
method validation
LC-MS/MS
mouse plasma
cotinine
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Summary:A sensitive analytical method was developed and validated for the quantification of cotinine in mouse plasma after exposure to smoke of 0.5, 1.0, and 1.5 commercially available cigarettes, using liquid chromatography tandem mass spectrometry. The method was validated over a linear concentration range of 0.075–20.0 ng/mL with the R2 value being higher than 0.99. Both the precision (coefficient of variation; %) and accuracy (relative error; %) were within acceptable criteria of <15%. The lower limit of quantification (LLOQ) for cotinine was 0.075 ng/mL with sufficient specificity, accuracy, and precision. Following exposure to 0.5, 1.0, and 1.5 cigarette smoke, it was observed that the AUC and the Cmax increased linearly as the doses increased. The pharmacokinetics of cotinine was found linear for the range of 0.5–1.5 commercial cigarette smoke. The quantification of the concentration of cotinine in mouse plasma after smoke exposure will facilitate future behavioral and toxicological experiments in animals and may prove useful in predicting cotinine levels in humans during smoking.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.4752