Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments

Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments

Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine Vol. 173; no. 3; pp. 384 - 389
Main Authors: Korolenya, V. A., Gavrilov, K. A., Sevost’ianova, K. S., Shevela, A. I., Filipenko, M. L., Smetanina, M. A.
Format: Journal Article
Language:English
Published: New York Springer US 01-07-2022
Springer
Springer Nature B.V
Subjects:
Alcohol
Analysis
Biology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Chloroform
Contamination
Deoxyribonuclease
DNA
Ethanol
Gene expression
Genes
Genetic transcription
Halides
Internal Medicine
Laboratory Medicine
Lithium
Lithium chloride
Lithium compounds
Pathology
Phenols
Reverse transcription
Ribonucleic acid
RNA
Veins & arteries
precipitation selectivity
lithium chloride
isopropanol
reverse transcription
RNA isolation
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Summary:Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).
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ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-022-05554-8