Timing embryo biopsy for PGD - before or after cryopreservation?

Timing embryo biopsy for PGD - before or after cryopreservation?

Objective: Pre-implantation genetic diagnosis (PGD) is required in order to screen and diagnose embryos of patients at risk of having a genetically affected offspring. A biopsy to diagnose the genetic profile of the embryo may be performed either before or after cryopreservation. The aim of this stu...

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Bibliographic Details
Published in:Gynecological endocrinology Vol. 32; no. 9; pp. 756 - 758
Main Authors: Shinar, S., Kornecki, N., Schwartz, T., Mey-Raz, N., Amir, H., Almog, B., Shavit, T., Hasson, J.
Format: Journal Article
Language:English
Published: England Taylor & Francis 01-09-2016
Subjects:
Adult
Biopsy
Biopsy - standards
blastomere
cryopreservation
Cryopreservation - standards
embryo
Embryo Transfer - standards
Female
freeze
Humans
PGD
Preimplantation Diagnosis - standards
Reproductive Techniques, Assisted - standards
Retrospective Studies
slow
Time Factors
freeze
slow
Biopsy
embryo
PGD
blastomere
cryopreservation
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Summary:Objective: Pre-implantation genetic diagnosis (PGD) is required in order to screen and diagnose embryos of patients at risk of having a genetically affected offspring. A biopsy to diagnose the genetic profile of the embryo may be performed either before or after cryopreservation. The aim of this study was to determine which biopsy timing yields higher embryo survival rates. Study design: Retrospective cohort study of all PGD patients in a public IVF unit between 2010 and 2013. Inclusion criteria were patients with good-quality embryos available for cryopreservation by the slow freezing method. Embryos were divided into two groups: biopsy before and biopsy after cryopreservation. The primary outcome was embryo survival rates post thawing. Results: Sixty-five patients met inclusion criteria. 145 embryos were biopsied before cryopreservation and 228 embryos were cryopreserved and biopsied after thawing. Embryo survival was significantly greater in the latter group (77% vs. 68%, p < 0.0001). Conclusion: Cryopreservation preceding biopsy results in better embryo survival compared to biopsy before cryopreservation.
Bibliography:ObjectType-Article-1
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ISSN:0951-3590
1473-0766
DOI:10.1080/09513590.2016.1177010