Search Results - "Kornberg, R.D."

Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae by Sayre, M.H, Tschochner, H, Kornberg, R.D

“…Yeast RNA polymerase II initiation factors a, b, e, and g were isolated from whole cell extract and found to be sufficient, when combined with bacterially…”
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Purification and characterization of yeast RNA polymerase II transcription factor b by Feaver, W.J. (Stanford University School of Medicine, Stanford, CA), Gileadi, O, Kornberg, R.D

“…Heat treatment of yeast nuclear extracts abolished the capacity to initiate transcription at RNA polymerase 11 promoters. Activity was restored by the addition…”
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Purification and properties of Saccharomyces cerevisiae RNA polymerase II general initiation factor a by Sayre, M.H, Tschochner, H, Kornberg, R.D

“…RNA polymerase II initiation factor a was purified to apparent homogeneity from yeast whole cell extract and consisted of two highly charged polypeptides with…”
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Structure of a Thiol Monolayer-Protected Gold Nanoparticle at 1.1 Angstrom Resolution by Jadzinsky, P.D., Calero, G., Ackerson, C.J., Bushnell, D.A., Kornberg, R.D.

“…Structural information on nanometer-sized gold particles has been limited, due in part to the problem of preparing homogeneous material. Here we report the…”
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Structural Basis of Transcription: Role of the Trigger Loop in Substrate Specificity And Catalysis by Wang, D., Bushnell, D.A., Westover, K.D., Kaplan, C.D., Kornberg, R.D., /Stanford U. School Med

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A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast by Cairns, B.R, Kim, Y.J, Sayre, M.H, Laurent, B.C, Kornberg, R.D

“…A complex containing the products of the SWI1/ADR6, SWI2/SNF2, SWI3, SNFS, and SNF6 genes and four additional polypeptides has been purified from extracts of…”
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Evidence for a mediator cycle at the initiation of transcription by Svejstrup, J.Q. (Stanford University School of Medicine, CA.), Li, Y, Fellows, J, Gnatt, A, Bjorklund, S, Kornberg, R.D

“…Free and elongating (DNA-bound) forms of RNA polymerase II were separated from yeast. Most cellular polymerase II was found in the elongating fraction, which…”
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Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules by Darst, S.A., Ahlers, M., Meller, P.H., Kubalek, E.W., Blankenburg, R., Ribi, H.O., Ringsdorf, H., Kornberg, R.D.

“…Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional…”
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Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae by Poon, David, Bai, Yu, Campbell, Allyson M., Bjorklund, Stefan, Kim, Young-Joon, Zhou, Sharleen, Kornberg, Roger D., Weil, P. Anthony

“…Although the mechanisms of transcriptional regulation by RNA polymerase II are apparently highly conserved from yeast to man, the identification of a yeast…”
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Transcription factor b (TFIIH) is required during nucleotide-excision repair in yeast by Wang, Z, Svejstrup, J.Q, Feaver, W.J, Wu, X, Kornberg, R.D, Friedberg, E.C

“…Nucleotide-excision repair (NER) is an important cellular defence mechanism against mutagenesis and carcinogenesis. The essential yeast genes RAD3 (ref. 2) and…”
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Structure of wild-type yeast RNA polymerase II and location of Rpb4 and Rpb7 by Jensen, G.J, Meredith, G, Bushnell, D.A, Kornberg, R.D

“…The three‐dimensional structure of wild‐type yeast RNA polymerase II has been determined at a nominal resolution of 24 Å. A difference map between this…”
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Two dissociable subunits of yeast RNA polymerase II stimulate the initiation of transcription at a promoter in vitro by Edwards, A M, Kane, C M, Young, R A, Kornberg, R D

“…RNA polymerase II lacking the fourth and seventh largest subunits (pol II delta 4/7) was purified from Saccharomyces cerevisiae strain rpb-4, in which the gene…”
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Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae by BUCHMAN, A. R, KIMMERLY, W. J, RINE, J, KORNBERG, R. D

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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A yeast transcriptional stimulatory protein similar to human PC4 by Henry, N.L. (Stanford University School of Medicine, Stanford, CA.), Bushnell, D.A, Kornberg, R.D

“…A yeast protein has been identified that stimulates basal transcription by RNA polymerase II, binds both single- and double-stranded DNA, and interacts with…”
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Crystal structure of yeast TATA-binding protein and model for interaction with DNA by Chasman, Daniel I., Flaherty, Kevin M., Sharp, Phillip A., Kornberg, Roger D.

“…The C-terminal 179-aa region of yeast (Saccharomyces cerevisiae) TATA-binding protein (TBP), phylogenetically conserved and sufficient for many functions,…”
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Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH: homology to human cyclin-dependent kinase activating kinase and IIH subunits by Feaver, W.J. (Stanford University School of Medicine, Stanford, CA.), Henry, N.L, Wang, Z, Wu, X, Svejstrup, J.Q, Bushnell, D.A, Friedberg, E.C, Kornberg, R.D

“…Genes for the Tfb2, Tfb3, and Tfb4 subunits of yeast RNA polymerase transcription factor IIH (TFIIH) are described. All three genes are essential for cell…”
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Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae by Gustafsson, Claes M., Myers, Lawrence C., Beve, Jenny, Spåhr, Henrik, Lui, Mary, Erdjument-Bromage, Hediye, Tempst, Paul, Kornberg, Roger D.

“…Mediator was isolated from yeast on the basis of its requirement for transcriptional activation in a fully defined system. We have now identified three new…”
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Two conformations of RNA polymerase II revealed by electron crystallography by Asturias, Francisco J, Meredith, Gavin D, Poglitsch, Claudia L, Kornberg, Roger D

“…A new two-dimensional crystal form of yeast RNA polymerase II was obtained in which the conformation of the enzyme appears “open”, allowing entry of DNA, as…”
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RNA polymerase transcription factor IIH holoenzyme from yeast by Svejstrup, J Q, Feaver, W J, LaPointe, J, Kornberg, R D

“…An RNA polymerase transcription factor IIH holoenzyme (holoTFIIH) has been resolved to near homogeneity from Saccharomyces cerevisiae. HoloTFIIH comprises the…”
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Effects of activation-defective TBP mutations on transcription initiation in yeast by Kim, T.K, Hashimoto, S, Kelleher, R.J. III, Flanagan, P.M, Kornberg, R.D, Horikoshi, M, Roeder, R.G

“…Transcription initiation by RNA polymerase II is affected by an ordered series of general factor interactions with core promoter elements (leading to basal…”
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