Search Results - "Kopanchuk, Sergei"

Zeta Potential of Extracellular Vesicles: Toward Understanding the Attributes that Determine Colloidal Stability by Midekessa, Getnet, Godakumara, Kasun, Ord, James, Viil, Janeli, Lättekivi, Freddy, Dissanayake, Keerthie, Kopanchuk, Sergei, Rinken, Ago, Andronowska, Aneta, Bhattacharjee, Sourav, Rinken, Toonika, Fazeli, Alireza

“…Extracellular vesicles (EVs), including exosomes and microvesicles (<200 nm), play a vital role in intercellular communication and carry a net negative surface…”
Get full text

Intracellular dynamics of the Sigma-1 receptor observed with super-resolution imaging microscopy by Kopanchuk, Sergei, Vavers, Edijs, Veiksina, Santa, Ligi, Kadri, Zvejniece, Liga, Dambrova, Maija, Rinken, Ago

“…Sigma-1 receptor (Sig1R) is an endoplasmic reticulum (ER)-related membrane protein, that forms heteromers with other cellular proteins. As the mechanism of…”
Get full text

Image-based cell-size estimation for baculovirus quantification by Laasfeld, Tõnis, Kopanchuk, Sergei, Rinken, Ago

“…Measurement of virus concentration is essential for effective virus-based transfection technologies. Here, we describe a user-friendly, image-based cell-size…”
Get full text

Tumor-penetrating peptide for systemic targeting of Tenascin-C by Lingasamy, Prakash, Tobi, Allan, Kurm, Kaarel, Kopanchuk, Sergei, Sudakov, Aleksander, Salumäe, Markko, Rätsep, Tõnu, Asser, Toomas, Bjerkvig, Rolf, Teesalu, Tambet

“…Extracellular matrix in solid tumors has emerged as a specific, stable, and abundant target for affinity-guided delivery of anticancer drugs. Here we describe…”
Get full text

Microfluidic production, stability and loading of synthetic giant unilamellar vesicles by Ernits, Mart, Reinsalu, Olavi, Yandrapalli, Naresh, Kopanchuk, Sergei, Moradpur-Tari, Ehsan, Sanka, Immanuel, Scheler, Ott, Rinken, Ago, Kurg, Reet, Kyritsakis, Andreas, Linko, Veikko, Zadin, Veronika

“…In advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a…”
Get full text

Crystal Structure-Guided Design of Bisubstrate Inhibitors and Photoluminescent Probes for Protein Kinases of the PIM Family by Nonga, Olivier E., Lavogina, Darja, Enkvist, Erki, Kestav, Katrin, Chaikuad, Apirat, Dixon-Clarke, Sarah E., Bullock, Alex N., Kopanchuk, Sergei, Ivan, Taavi, Ekambaram, Ramesh, Viht, Kaido, Knapp, Stefan, Uri, Asko

“…We performed an X-ray crystallographic study of complexes of protein kinase PIM-1 with three inhibitors comprising an adenosine mimetic moiety, a linker, and a…”
Get full text

PL1 Peptide Engages Acidic Surfaces on Tumor-Associated Fibronectin and Tenascin Isoforms to Trigger Cellular Uptake by Lingasamy, Prakash, Põšnograjeva, Kristina, Kopanchuk, Sergei, Tobi, Allan, Rinken, Ago, General, Ignacio J, Asciutto, Eliana K, Teesalu, Tambet

“…Tumor extracellular matrix (ECM) is a high-capacity target for the precision delivery of affinity ligand-guided drugs and imaging agents. Recently, we…”
Get full text

Dissection of Protein Kinase Pathways in Live Cells Using Photoluminescent Probes: Surveillance or Interrogation? by Lavogina, Darja, Kopanchuk, Sergei, Viht, Kaido

“…Protein kinases catalyze phosphorylation, a small yet crucial modification that affects participation of the substrate proteins in the intracellular signaling…”
Get full text

Characterizing the bio-functionalization of gold surface with total internal reflection fluorescence (TIRF) microscopy by Ehrminger, Robin, Kopanchuk, Sergei, Kivirand, Kairi, Romann, Tavo, Rinken, Toonika, Min, Mart, Rinken, Ago

“…Quality of bioactive surface is crucial for achieving the required sensitivity and selectivity of biosensing systems. Numerous methods are available for the…”
Get full text

Assays with Detection of Fluorescence Anisotropy: Challenges and Possibilities for Characterizing Ligand Binding to GPCRs by Rinken, Ago, Lavogina, Darja, Kopanchuk, Sergei

“…Binding of fluorescent ligands (tracers) to their target receptors can be directly monitored over time, as the binding of a low-molecular-weight (LMW) tracer…”
Get full text

Implementation of fluorescence anisotropy-based assay for the characterization of ligand binding to dopamine D1 receptors by Allikalt, Anni, Kopanchuk, Sergei, Rinken, Ago

“…Dopamine receptors, which belong to the family of G protein-coupled receptors, are very substantial regulators in the brain and therefore important targets in…”
Get full text

Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live‐cell microscopy by Allikalt, Anni, Laasfeld, Tõnis, Ilisson, Mihkel, Kopanchuk, Sergei, Rinken, Ago

“…We implemented an assay combining fluorescence and bright‐field microscopy to measure ligand binding to dopamine D3 receptors in live mammalian cells. For…”
Get full text

Quantitative analysis of fluorescent ligand binding to dopamine D 3 receptors using live-cell microscopy by Allikalt, Anni, Laasfeld, Tõnis, Ilisson, Mihkel, Kopanchuk, Sergei, Rinken, Ago

“…Dopamine receptors are G protein-coupled receptors that have several essential functions in the central nervous system. A better understanding of the…”
Get full text

Precision Targeting of Tumor Macrophages with a CD206 Binding Peptide by Scodeller, Pablo, Simón-Gracia, Lorena, Kopanchuk, Sergei, Tobi, Allan, Kilk, Kalle, Säälik, Pille, Kurm, Kaarel, Squadrito, Mario Leonardo, Kotamraju, Venkata Ramana, Rinken, Ago, De Palma, Michele, Ruoslahti, Erkki, Teesalu, Tambet

“…Tumor-associated macrophages (TAMs) expressing the multi-ligand endocytic receptor mannose receptor (CD206/MRC1) contribute to tumor immunosuppression,…”
Get full text

Dynamics of ligand binding to GPCR: Residence time of melanocortins and its modulation by Rinken, Ago, Veiksina, Santa, Kopanchuk, Sergei

“…[Display omitted] Ligand binding dynamics and the concept of drug-target residence time are essential factors in the development of novel drugs. Conventional…”
Get full text

Characterization of ligand binding to melanocortin 4 receptors using fluorescent peptides with improved kinetic properties by Link, Reet, Veiksina, Santa, Rinken, Ago, Kopanchuk, Sergei

“…Melanocortin 4 (MC4) receptors are important drug targets as they regulate energy homeostasis, eating behaviour and sexual functions. The ligand binding…”
Get full text

Phthalate monoesters affect membrane fluidity and cell-cell contacts in endometrial stromal adherent cell lines and spheroids by Lavogina, Darja, Kask, Keiu, Kopanchuk, Sergei, Visser, Nadja, Laws, Mary, Flaws, Jodi A., Kallak, Theodora Kunovac, Olovsson, Matts, Damdimopoulou, Pauliina, Salumets, Andres

“…Phthalate monoesters have been identified as endocrine disruptors in a variety of models, yet understanding of their exact mechanisms of action and molecular…”
Get full text

Enhanced osteointegration and osteogenesis of osteoblast cells by laser-induced surface modification of Ti implants by Gnilitskyi, Iaroslav, Dolgov, Leonid, Tamm, Aile, Ferraria, Ana Maria, Diedkova, Kateryna, Kopanchuk, Sergei, Tsekhmister, Yaroslav, Veiksina, Santa, Polewczyk, Vincent, Pogorielov, Maksym

“…Dental and orthopedic implants have become routine medical technologies for tooth replacement and bone fixation. Despite significant progress in implantology,…”
Get full text

Structure-Based Design of High-Affinity Fluorescent Probes for the Neuropeptide Y Y1 Receptor by Müller, Christoph, Gleixner, Jakob, Tahk, Maris-Johanna, Kopanchuk, Sergei, Laasfeld, Tõnis, Weinhart, Michael, Schollmeyer, Dieter, Betschart, Martin U, Lüdeke, Steffen, Koch, Pierre, Rinken, Ago, Keller, Max

“…The recent crystallization of the neuropeptide Y Y1 receptor (Y1R) in complex with the argininamide-type Y1R selective antagonist UR-MK299 (2) opened up a new…”
Get full text

The constitutive activity of melanocortin‐4 receptors in cAMP pathway is allosterically modulated by zinc and copper ions by Link, Reet, Veiksina, Santa, Tahk, Maris‐Johanna, Laasfeld, Tõnis, Paiste, Päärn, Kopanchuk, Sergei, Rinken, Ago

“…Melanocortin‐4 receptors (MC4R) are unique among G‐protein‐coupled receptors (GPCRs) as they have endogenous ligands that can exhibit inverse agonistic…”
Get full text