Resistance of Nile tilapia fed with Padina boergesenii extract to Pseudomonas putida infection

The aim of this research was to estimate the immunopotentiation effect of brown algae Padina boergesenii water extract on Nile tilapia, Oreochromis niloticus through resistance to Pseudomonas putida infection. Gas Chromatography Mass Spectrometry was utilized to characterize the seaweed phytoconstit...

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Published in:	BMC veterinary research Vol. 20; no. 1; pp. 281 - 18
Main Authors:	Bakry, Karima A, Nasr, Mahmoud, Al-Amgad, Zeinab, Kondos, Ereen, Kondos, Malak K N, Mehanny, Pierre E, Alghamdi, Abdullah A A, Khormi, Mohsen A, Abd-ElHafeez, Hanan H, Emeish, Walaa F A
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 29-06-2024 BioMed Central BMC
Subjects:	Algae Animal Feed - analysis Animals Antibiotics Aquaculture Bacterial infections Brown algae Chromatography Cichlids Defensins Diet Diet - veterinary Dietary Supplements Disease Resistance - drug effects Experiments Fish Fish Diseases - microbiology Fish Diseases - prevention & control Gas chromatography GC-MS analysis Gene expression Health aspects Immune response Immune system Infections Innate immunity Laboratory animals Mass spectrometry Mass spectroscopy Medical research Methods Nutrition research Oreochromis niloticus Oxidative stress Padina boergesenii Peptides Phaeophyceae - chemistry Physiological aspects Plant Extracts - administration & dosage Plant Extracts - chemistry Plant Extracts - pharmacology Pseudomonas Infections - drug therapy Pseudomonas Infections - veterinary Pseudomonas putida Pseudomonas putida - drug effects Seaweeds Serum biochemical parameters Tilapia Veterinary medicine Water Egypt Oxidative stress Oreochromis niloticus Brown algae Bacterial challenge GC-MS analysis Serum biochemical parameters Gene expression
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Summary:	The aim of this research was to estimate the immunopotentiation effect of brown algae Padina boergesenii water extract on Nile tilapia, Oreochromis niloticus through resistance to Pseudomonas putida infection. Gas Chromatography Mass Spectrometry was utilized to characterize the seaweed phytoconstituents. One hundred and twenty-six fish were divided in triplicates into two equal groups corresponding to two diet variants that used to feed Nile tilapia for 20 successive days: a basal (control), and P. boergesenii water extract supplemented group. Fish samples were collected at 10-days intervals throughout the experiment. Serum biochemical constituents, total antioxidant capacity (TAC), and some immune related genes expression of the spleen and intestinal tissues of experimental fish were studied, as well as histological examination of fish immune tissues. Moreover, following 20 days of feeding, the susceptibility of Nile tilapia to P. putida infection was evaluated to assess the protective effect of the used extract. The findings indicated that the studied parameters were significantly increased, and the best immune response profiles were observed in fish fed P. boergesenii water extract for 20 successive days. A bacterial challenge experiment using P. putida resulted in higher survival within the supplemented fish group than the control. Thus, the lowered post-challenge mortality of the fish may be related to the protection provided by the stimulation of the innate immune system, reduced oxidative stress by higher activity of TAC, and elevated levels of expression of iterleukin-1beta (IL-1β), beta-defensin (β-defensin), and natural killer-lysin (NKl). Moreover, the constituents of the extract used showed potential protective activity for histological features of the supplemented fish group when compared to the control. Collectively, this study presents a great insight on the protective role of P. boergesenii water extract as an additive in Nile tilapia feed which suggests its potential for improving the immune response against P. putida infection.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1746-6148 1746-6148
DOI:	10.1186/s12917-024-04115-7